I performed Western blot with NFkB p65 Antibody (NB100-2176) using brain and liver tissues extracts. My question: is it possible that NF-kB-Ikb complex could be detected by this antibody? Because I found 2 bands in the Western blot results, one is located at 65 and the other above 100 kD.

Name: RelA/NFkB p65 Antibody
Brand: Novus Biologicals
SKU: NB100-2176
Rating: 5 (3 reviews)

Question

Accepted Answer

It possible that NB100-2176 could detect the entire NFkB complex in addition to just the p65 protein. If your samples were not completely reduced and denatured, they could have remained bound in your gel. The band above 100 kDa could also be an aggregate of NFkB. You may consider probing that same membrane for a different NFkB complex protein that you would expect to remain bound under these conditions. A negative signal in this case would tell you that it's an aggregate of NFkB, or maybe that it's a non-specific band. Did you run a negative control?

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, ICC/IF, IHC, IP, ChIP
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Concentration	0.2 mg/ml

Immunogen	Synthetic peptide corresponding to the C-terminus of human NFkB p65.
Localization	Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I kappa B).
Specificity	Reacts with NFkB p65
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	RELA
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C. Do not freeze.
Buffer	PBS and 0.2% gelatin.
Preservative	0.1% Sodium Azide
Concentration	0.2 mg/ml
Purity	Immunogen affinity purified

Publications using NB100-2176	Applications	Species
Kassab RB, Elhenawy AA, AbdulrahmanTheyab et al. Modulation of inflammatory, oxidative, and apoptotic stresses mediates the renoprotective effect of daidzein against glycerol-induced acute kidney injury in rats Environmental science and pollution research international 2023-11-03 [PMID: 37919499]
Ozturk I, Elbe H, Bicer Y et al. Therapeutic role of melatonin on acrylamide-induced hepatotoxicity in pinealectomized rats: Effects on oxidative stress, NF-?B signaling pathway, and hepatocellular proliferation Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023-02-11 [PMID: 36780936] (IHC-P, Rat)	IHC-P	Rat
Li L, Ma H, Zhang Y et al. Protocatechuic acid reverses myocardial infarction mediated by b-adrenergic agonist via regulation of Nrf2/HO-1 pathway, inflammatory, apoptotic, and fibrotic events Journal of biochemical and molecular toxicology 2023-01-02 [PMID: 36593721]
Salama B, Alzahrani K, Alghamdi K et al. Silver nanoparticles enhance oxidative stress, inflammation, and apoptosis in liver and kidney tissues: Potential protective role of thymoquinone Research Square 2022-07-13 [PMID: 36018545]
Netzel L Respiratory Biomarkers in Kv1. 1 Knockout Mice, a Model for Temporal Lobe Epilepsy Thesis 2020-01-01
Mohamed K, Abdelfattah M, El-khadragy M et al. Rutin-loaded selenium nanoparticles modulated the redox status, inflammatory, and apoptotic pathways associated with pentylenetetrazole-induced epilepsy in mice Green Processing and Synthesis 2023-01-26 (ELISA, Mouse)	ELISA	Mouse
Pereira, M;Ramalho, T;Andrade, WA;Durso, DF;Souza, MC;Fitzgerald, KA;Golenbock, DT;Silverman, N;Gazzinelli, RT; The IRAK1/IRF5 axis initiates IL-12 response by dendritic cells and control of Toxoplasma gondii infection Cell reports 2024-02-15 [PMID: 38367238]
Pereira M, Durso DF, Bryant CE et al. The IRAK4 scaffold integrates TLR4-driven TRIF and MYD88 signaling pathways Cell reports 2022-08-16 [PMID: 35977521] (WB, Mouse) Details: Dilutions: 1:1000	WB	Mouse
Desai M, Stiles L, Torsoni AS et al. TNF alpha-Induced Oxidative Stress and Mitochondrial Dysfunction Alter Hypothalamic Neurogenesis and Promote Appetite Versus Satiety Neuropeptide Expression in Mice Brain sciences 2022-07-09 [PMID: 35884707] (WB, Mouse)	WB	Mouse
Zheng X, Wang K, Pan L et al. Innate Immune Responses to RSV Infection Facilitated by OGG1, an Enzyme Repairing Oxidatively Modified DNA Base Lesions Journal of Innate Immunity 2022-05-05 [PMID: 35512649]
Al Omairi N, Albrakati A, Alsharif K et al. Selenium Nanoparticles with Prodigiosin Rescue Hippocampal Damage Associated with Epileptic Seizures Induced by Pentylenetetrazole in Rats Biology 2022-02-23 [PMID: 35336729]
Mou KJ, Shen KF, Li YL et al. Adenosine A2A Receptor in Bone Marrow-Derived Cells Mediated Macrophages M2 Polarization via PPAR gamma-P65 Pathway in Chronic Hypoperfusion Situation Frontiers in aging neuroscience 2022-01-03 [PMID: 35046793] (WB, Mouse)	WB	Mouse
Gutziet O, Iluz R, Ben Asher H et al. Maternal N-Acetyl-Cysteine Prevents Neonatal Hypoxia-Induced Brain Injury in a Rat Model International journal of molecular sciences 2021-12-20 [PMID: 34948425] (WB, Rat)	WB	Rat
Suzanne Faure-Dupuy, Tobias Riedl, Maude Rolland, Zoheir Hizir, Florian Reisinger, Katharina Neuhaus, Svenja Schuehle, Caroline Remouchamps, Nicolas Gillet, Maximilian Schönung, Mira Stadler, Jochen Wettengel, Romain Barnault, Romain Parent, Linda Christina Schuster, Rayan Farhat, Sandra Prokosch, Corinna Leuchtenberger, Rupert Öllinger, Thomas Engleitner, Karsten Rippe, Roland Rad, Kristian Unger, Darjus Tscharahganeh, Daniel B. Lipka, Ulrike Protzer, David Durantel, Julie Lucifora, Emmanuel Dejardin, Mathias Heikenwälder Control of APOBEC3B induction and cccDNA decay by NF-kappa B and miR-138-5p JHEP Reports 2021-08-25 [PMID: 34704004]
Brandetti E, Focaccetti C, Pezzolo A et al. Enhancement of Neuroblastoma NK-Cell-Mediated Lysis through NF-kB p65 Subunit-Induced Expression of FAS and PVR, the Loss of Which Is Associated with Poor Patient Outcome Cancers (Basel) 2021-08-29 [PMID: 34503178] (Flow Cytometry, Western Blot)	Flow Cytometry, Western Blot
Fernanda S, Caterina C, Valentina D Et Al. Platelet lysate converts M (IFN gamma+LPS) macrophages in CD206+ TGF-beta+ arginase+ M2-like macrophages that affect fibroblast activity and T lymphocyte migration Journal of tissue engineering and regenerative medicine 2021-07-26 [PMID: 34311512]
Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (WB, Mouse) Details: Citation using the PE format of this antibody.	WB	Mouse
Li C, Zhao W, Pan X et al. LncRNA KTN1-AS1 promotes the progression of non-small cell lung cancer via sponging of miR-130a-5p and activation of PDPK1 Oncogene 2020-08-20 [PMID: 32820252] (WB, Human)	WB	Human
Shah R, Makarem N, Emin M et al. Mediterranean diet components are linked to greater endothelial function and lower inflammation in a pilot study of ethnically diverse women Nutrition Research 2020-01-01 [PMID: 32045786] (Human)		Human
Wang F, Zhang C, Ge W, Zhang G. Up-regulated CST5 inhibits bone resorption and activation of osteoclasts in rat models of osteoporosis via suppression of the NF-?B pathway Journal of Cellular and Molecular Medicine 2019-10-01 [PMID: 31402549] (Western Blot)	Western Blot
Zhao L, Chen S et al. Callistephin enhances the protective effects of isoflurane on microglial injury through downregulation of inflammation and apoptosis. Mol Med Rep 2019-01-07 [PMID: 31180517] (WB, Mouse)	WB	Mouse
Chia-Feng Kuo, Chih-Hsuan Hsieh, Wun-Yuan Lin. Proteomic response of LAP-activated RAW 264.7 macrophages to the anti-inflammatory property of fungal ergosterol.. Food chemistry. 2011-01-01
Kim HJ, Joe Y, Chen Y et al. Carbon monoxide attenuates amyloidogenesis via down-regulation of NF-kB-mediated BACE1 gene expression. Aging Cell. 2018-11-09 [PMID: 30411846] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Sheng W, Mao H, Wang C et al. Dehydrocostus Lactone Enhances Chemotherapeutic Potential of Doxorubicin in Lung Cancer by Inducing Cell Death and Limiting Metastasis Med. Sci. Monit. 2018-11-02 [PMID: 30388099] (WB, Human)	WB	Human
Aggarwal B, Makarem N, Shah R et al. Effects of Inadequate Sleep on Blood Pressure and Endothelial Inflammation in Women: Findings From the American Heart Association Go Red for Women Strategically Focused Research Network J Am Heart Assoc 2018-06-09 [PMID: 29886425] (Human)		Human
Zaky A, Bouali-Benazzouz R, Favereaux A et al. APE1/Ref-1 redox function contributes to inflammatory pain sensitization Exp. Neurol. 2018-05-14 [PMID: 29772245] (Rat)		Rat
Li GF, Zhuang SJ, Li GC. Inhibition of microRNA-34a protects against propofol anesthesia-induced neurotoxicity and cognitive dysfunction via the MAPK/ERK signaling pathway. Neurosci. Lett. 2018-03-22 [PMID: 29578002] (WB, Human)	WB	Human
Ribes D, Casemayou A, El Hachem H et al. Asymptomatic circulating T-cell clone cause renal polymorphic inflammatory fibrosis. Clin. Exp. Nephrol. 2016-12-26 [PMID: 28018996] (WB, Human)	WB	Human
Liu JY, Guo F, Wu HL et al. Midazolam anesthesia protects neuronal cells from oxidative stress-induced death via activation of the JNK-ERK pathway Mol Med Rep 2017-01-01 [PMID: 27959401] (WB, Mouse)	WB	Mouse
Li J, Luco AL, Ochietti B et al. Tumoral Vitamin D Synthesis by CYP27B1 1-alpha-Hydroxylase Delays Mammary Tumor Progression in the PyMT-MMTV Mouse Model and Its Action Involves NF-kB Modulation. Endocrinology. 2016-04-27 [PMID: 27119753] (ICC/IF, Mouse)	ICC/IF	Mouse
Sahbaz A, Aynioglu O, Isik H et al. Cholecalciferol (vitamin D3) prevents postoperative adhesion formation by inactivating the nuclear factor kappa B pathway: a randomized experimental study. J. Surg. Res. 2015-05-14 [PMID: 26038248] (IHC-P, Rat)	IHC-P	Rat
Zhu Shijun, Moore Terry W, Morii Nao et al. Synthetic curcumin analog UBS109 inhibits the growth of head and neck squamous cell carcinoma xenografts. Curr Cancer Drug Targets. 2014-05-01 [PMID: 24628271]
Zaky A, Mohammad B, Moftah M et al. Apurinic/apyrimidinic endonuclease 1 is a key modulator of aluminum-induced neuroinflammation. BMC Neurosci 2013-03-19 [PMID: 23497276] (WB, Rat)	WB	Rat
Zhu S, Moore TW, Lin X et al. Synthetic curcumin analog EF31 inhibits the growth of head and neck squamous cell carcinoma xenografts. Integr Biol (Camb). 2012-06-01 [PMID: 22532032] (IHC-P, WB, Human)	IHC-P, WB	Human
Kang HB, Kim YE, Kwon HJ, Sok DE, Lee Y. Enhancement of NF-kappaB expression and activity upon differentiation of human embryonic stem cell line SNUhES3. Stem Cells Dev;16(4):615-23. 2007-08-01 [PMID: 17784835]
Fan Z, Soder S, Oehler S, Fundel K, Aigner T. Activation of interleukin-1 signaling cascades in normal and osteoarthritic articular cartilage. Am J Pathol;171(3):938-46. 2007-09-01 [PMID: 17640966]
Longpre F, Garneau P, Christen Y, Ramassamy C. Protection by EGb 761 against beta-amyloid-induced neurotoxicity: involvement of NF-kappaB, SIRT1, and MAPKs pathways and inhibition of amyloid fibril formation. Free Radic Biol Med;41(12):1781-94. 2006-12-15 [PMID: 17157181]
Starkey JM, Haidacher SJ, LeJeune WS et al. Diabetes-induced activation of canonical and noncanonical nuclear factor-kappaB pathways in renal cortex. Diabetes 2006-05-01 [PMID: 16644679]
Lee KW, Lee Y, Kim DS, Kwon HJ. Direct role of NF-kappaB activation in Toll-like receptor-triggered HLA-DRA expression. Eur J Immunol;36(5):1254-66. 2006-05-01 [PMID: 16619292]
Popov Y, Patsenker E, Bauer M, Niedobitek E, Schulze-Krebs A, Schuppan D. Halofuginone induces matrix metalloproteinases in rat hepatic stellate cells via activation of p38 and NFkappaB. J Biol Chem 281(22):15090-8. 2006-01-01 [PMID: 16489207]
Lee KW, Lee Y, Kwon HJ Kim DS. Sp1-associated activation of macrophage inflammatory protein-2 promoter by CpG-oligodeoxynucleotide and lipopolysaccharide. Cell and Molecular Life Sciences 62:188-198. 2005-01-01 [PMID: 15666090]
Stjernholm-Vladic Y, Stygar D, Mansson C, Masironi B, Akerberg S, Wang H, Ekman-Ordeberg G, Sahlin L. Factors involved in the inflammatory events of cervical ripening in humans. Reproductive Biology & Endocrinology 2:74. 2004-01-01 [PMID: 15500686]
Joo SS, Won TJ, Lee do I. Potential role of ursodeoxycholic acid in suppression of nuclear factor kappa B in microglial cell line (BV-2). Arch Pharm Res;27(9):954-60. 2004-09-01 [PMID: 15473667]
Leitch D, Barrans SL, Jack AS, Owen RG. Dysregulation of apoptosis in Waldenstrom's macroglobulinemia does not involve nuclear factor kappa B activation. Semin Oncol;30(2):161-4. 2003-04-01 [PMID: 12720128]
Szybinski Z, Pach J, Bogusz M et al. The measurement of exposition and results of prophylactic studies in workers chronically exposed to action of mercury vapours (author's transl). Przegl Lek 1905-06-01 [PMID: 542624]
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RelA/NFkB p65 Antibody

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Gene Symbol	RELA
Entrez	5970(Human) 18033(Mouse) 81736(Rat)
Uniprot	Q04206()

	Armen Petrosyan 11/19/2024
Application:	IF
Species:	Mouse

	Armen Petrosyan 11/07/2024
Application:	IHC-P
Species:	Mouse

	Verified Customer 08/31/2017
Application:	WB
Species:	Human