| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Chicken |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant rat Neurofascin Ile25-Ala1031 Accession # NP_446361 |
| Specificity | Detects human, mouse, rat Neurofascin in Western blots and rat Neurofascin in direct ELISAs. |
| Source | N/A |
| Isotype | IgY |
| Clonality | Polyclonal |
| Host | Chicken |
| Gene | NFASC |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Neurofascin 155 (NF155) is a type I transmembrane glycoprotein that belongs to the L1CAM family of cell adhesion proteins (1, 2). The rat NF155 cDNA encodes a 1240 amino acid (aa) precursor that contains a 24 aa signal sequence, a 1086 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 109 aa cytoplasmic domain. The ECD consists of six Ig-like domains and four fibronectin type III repeats, the second of which has an RGD motif. A splice variant of Neurofascin, NF186, lacks the RGD-containing fibronectin type III domain but instead has a mucin-like domain and an additional non-RGD fibronectin type III domain (3). Within shared regions of the ECD, rat NF155 shares 45% and 39% aa sequence identity with rat Nr-CAM and L1CAM, respectively, and 98% aa sequence identity with human and mouse NF155. NF155 is transiently expressed by oligodendrocytes at the onset of axon myelination, whereas NF186 is neuronally expressed in nodes of Ranvier (4‑6). Clustering of NF155 in paranodal oligodengroglia lipid raft domains is stabilized by dimerization of its cytoplasmic domains and association with intracellular ankyrin (6‑9). NF155 interacts with axonal contactin and plays a role in node of Ranvier formation and the establishment of saltatory conduction (5, 9‑12). The ECD of NF155 is cleaved from oligodengroglia membranes by metalloproteases, a process which is required for NF155 transport from the glial cell body to the axoglial junction (13). In addition to distinct expression patterns, Neurofascin isoforms have different functional properties. NF155 promotes neuronal adhesion and neurite outgrowth, whereas NF186 inhibits neuronal adhesion (4, 7, 13).
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
YONGJIE LAI |
IF | Human | 07/16/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
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![P0T124M mutation alters SLI morphology, length,&distribution. (D) Quantification shows an increased %age of SLIs in nerves from mice harboring the P0T124M mutation (MpzT124M) at 2, 6, 12,&18 months of age. One-way ANOVA [2 month old: F (2, 10) = 29.86, p<0.0001; 6 month old: F (2, 9) = 41.60, p<0.0001, 12 month old: F (2, 6) = 34.35, p = 0.0005; 18 month old: F (2, 8) = 21.71, p = 0.0006]. Representative confocal pictures of sciatic teased fibers stained with FITC-phalloidin (ACTIN, green) (E, I,&M) or anti-MAG antibodies (Q) (green)&anti-pan-NFASC antibodies (red) at 2 (E), 6 (I),&12 (M&Q) months of age. SLI morphology is disrupted in MpzT124M mice. Scale bar: 10 μm. Measurements of SLI length at 2 (F), 6 (J),&12 (N&R) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 32.16, p <0.0001; 6 month old: F (2,6) = 11.18, p = 0.0095 12 month old: F (2,9) = 6.447, p = 0.0183]. Measurements of the distance between adjacent SLI at 2 (G), 6 (K),&12 (O&S) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 24.90, p = 0.0002; 6 month old: F (2,6) = 9.382, p = 0.0142; 12 month old: F (2,9) = 13.04, p = 0.0022]. Quantifications of SLI number per 100 μm at 2 (H), 6 (L),&12 (P&T) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 20.29, p = 0.0005; 6 month old: F (2,6) = 22.81, p = 0.016; 12 month old: F (2,9) = 28.25, p = 0.0001]. At least 207 SLI per genotype quantified at each time point. n (animals) ≥ 3 per genotype. *p < 0.05, **p < 0.01, ***p < 0.001 by multiple-comparisons Tukey’s post hoc tests after Nested one-way ANOVA (D, F, G, H, J, K, L, R, S,&T) or by two-tailed Student’s t test (N, O,&P). Graphs indicate means ± SEMs. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36350884), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3235_human-mouse-rat-neurofascin-affinity-purified-polyclonal-ab-8120255541769.jpg "P0T124M mutation alters SLI morphology, length,&distribution. (D) Quantification shows an increased %age of SLIs in nerves from mice harboring the P0T124M mutation (MpzT124M) at 2, 6, 12,&18 months of age. One-way ANOVA [2 month old: F (2, 10) = 29.86, p<0.0001; 6 month old: F (2, 9) = 41.60, p<0.0001, 12 month old: F (2, 6) = 34.35, p = 0.0005; 18 month old: F (2, 8) = 21.71, p = 0.0006]. Representative confocal pictures of sciatic teased fibers stained with FITC-phalloidin (ACTIN, green) (E, I,&M) or anti-MAG antibodies (Q) (green)&anti-pan-NFASC antibodies (red) at 2 (E), 6 (I),&12 (M&Q) months of age. SLI morphology is disrupted in MpzT124M mice. Scale bar: 10 μm. Measurements of SLI length at 2 (F), 6 (J),&12 (N&R) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 32.16, p <0.0001; 6 month old: F (2,6) = 11.18, p = 0.0095 12 month old: F (2,9) = 6.447, p = 0.0183]. Measurements of the distance between adjacent SLI at 2 (G), 6 (K),&12 (O&S) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 24.90, p = 0.0002; 6 month old: F (2,6) = 9.382, p = 0.0142; 12 month old: F (2,9) = 13.04, p = 0.0022]. Quantifications of SLI number per 100 μm at 2 (H), 6 (L),&12 (P&T) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 20.29, p = 0.0005; 6 month old: F (2,6) = 22.81, p = 0.016; 12 month old: F (2,9) = 28.25, p = 0.0001]. At least 207 SLI per genotype quantified at each time point. n (animals) ≥ 3 per genotype. *p < 0.05, **p < 0.01, ***p < 0.001 by multiple-comparisons Tukey’s post hoc tests after Nested one-way ANOVA (D, F, G, H, J, K, L, R, S,&T) or by two-tailed Student’s t test (N, O,&P). Graphs indicate means ± SEMs. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36350884), licensed under a CC-BY license. Not internally tested by R&D Systems.")
![P0T124M mutation alters nodes and paranodes.Representative confocal pictures of sciatic nerve teased fibers stained with antibodies against the paranodal marker CASPR (red) and the paranodal and nodal marker pan-Neurofascin (NFASC; green) at 2 (A) and 12 (E) months of age. Scale bars: 5 μm. CASPR length quantifications at 2 (B) and 12 (F) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 7.009, p = 0.0146; 12 month old: F (2,7) = 35.47, p = 0.0002]. Relative frequency distributions of paranodal (CASPR) length at 2 (C) and 12 (G) months of age. Nodal length quantifications at 2 (D) and 12 (H) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 1.122, p = 0.3671, 12 month old: F (2,7) = 8.012, p = 0.0155]. At least 200 paranodes and 100 nodes per genotype were quantified at each time point. n (animals) ≥ 3 per genotype. (I) Electron micrographs of ultrathin longitudinal WT and MpzT124M/T124M sciatic nerve section at 12 months of age. In (I) electron micrographs represent nodes of WT and MpzT124M/T124M sciatic nerves. (J) The quantification of nodal length shows significant widening of the node in MpzT124M/T124M. Magnifications of (I) show disorganized paranonal loops in MpzT124M/T124M but well organized in WT. n (animals) ≥ 3 per genotype, 7 to 11 nodes were counted per animals, for a total of 27 and 28 counted by genotype. Scale bare: top panel 1 μm, middle panel 500 nm, bottom panel 200 nm. ***p < 0.001 by multiple-comparisons Tukey’s post hoc tests after Nested one-way ANOVA (B, F, D, and H) or Nested two-tailed Student’s t test (J). Graphs indicate means ± SEMs. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36350884), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3235_human-mouse-rat-neurofascin-affinity-purified-polyclonal-ab-8120255552818.jpg "P0T124M mutation alters nodes and paranodes.Representative confocal pictures of sciatic nerve teased fibers stained with antibodies against the paranodal marker CASPR (red) and the paranodal and nodal marker pan-Neurofascin (NFASC; green) at 2 (A) and 12 (E) months of age. Scale bars: 5 μm. CASPR length quantifications at 2 (B) and 12 (F) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 7.009, p = 0.0146; 12 month old: F (2,7) = 35.47, p = 0.0002]. Relative frequency distributions of paranodal (CASPR) length at 2 (C) and 12 (G) months of age. Nodal length quantifications at 2 (D) and 12 (H) months of age. Nested one-way ANOVA [2 month old: F (2,9) = 1.122, p = 0.3671, 12 month old: F (2,7) = 8.012, p = 0.0155]. At least 200 paranodes and 100 nodes per genotype were quantified at each time point. n (animals) ≥ 3 per genotype. (I) Electron micrographs of ultrathin longitudinal WT and MpzT124M/T124M sciatic nerve section at 12 months of age. In (I) electron micrographs represent nodes of WT and MpzT124M/T124M sciatic nerves. (J) The quantification of nodal length shows significant widening of the node in MpzT124M/T124M. Magnifications of (I) show disorganized paranonal loops in MpzT124M/T124M but well organized in WT. n (animals) ≥ 3 per genotype, 7 to 11 nodes were counted per animals, for a total of 27 and 28 counted by genotype. Scale bare: top panel 1 μm, middle panel 500 nm, bottom panel 200 nm. ***p < 0.001 by multiple-comparisons Tukey’s post hoc tests after Nested one-way ANOVA (B, F, D, and H) or Nested two-tailed Student’s t test (J). Graphs indicate means ± SEMs. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36350884), licensed under a CC-BY license. Not internally tested by R&D Systems.")
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