Home » A-431 » A-431 Lysates » A-431 Whole Cell Lysate
Novus Biologicals products are now on bio-techne.com

A-431 Whole Cell Lysate

 Submit a Review

Images

 
There are currently no images for A-431 Lysate (NB820-59461).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
5.0 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

A-431 Whole Cell Lysate Summary

Description
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Specificity
Total Protein - Human Tumor Cell Line: A431

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Control
14-3-3 sigma/Stratifin Antibody
14-3-3 sigma/Stratifin Antibody
EGFR [p Tyr1173] Antibody [Unconjugated]
EGFR [p Tyr1173] Antibody [Unconjugated]

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Skin
Protein State
Native
Subcellular Fraction
Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for A-431 Lysate (NB820-59461) (0)

There are no publications for A-431 Lysate (NB820-59461).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for A-431 Lysate (NB820-59461) (0)

There are no reviews for A-431 Lysate (NB820-59461). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for A-431 Lysate (NB820-59461) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

14-3-3 sigma/Stratifin Antibody
14-3-3 sigma/Stratifin Antibody
EGFR [p Tyr1173] Antibody [Unconjugated]
EGFR [p Tyr1173] Antibody [Unconjugated]

Additional A-431 Products

Array NB820-59461

Blogs on A-431

There are no specific blogs for A-431, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our A-431 Whole Cell Lysate and receive a gift card or discount.