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A-431 Whole Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
5.0 mg/ml

Order Details

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A-431 Whole Cell Lysate Summary

Description
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Specificity
Total Protein - Human Tumor Cell Line: A431

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Control
14-3-3 sigma/Stratifin Antibody
14-3-3 sigma/Stratifin Antibody
EGFR [p Tyr1173] Antibody [Unconjugated]
EGFR [p Tyr1173] Antibody [Unconjugated]

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Skin
Protein State
Native
Subcellular Fraction
Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for A-431 Lysate (NB820-59461) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

14-3-3 sigma/Stratifin Antibody
14-3-3 sigma/Stratifin Antibody
EGFR [p Tyr1173] Antibody [Unconjugated]
EGFR [p Tyr1173] Antibody [Unconjugated]

Additional A-431 Products

Array NB820-59461

Blogs on A-431

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Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

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