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Recombinant Mouse TGF-beta 2 Protein

 (27 Publications)
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Carrier Free
7346-B2-005/CF
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Recombinant Mouse TGF-beta 2 Protein Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.05‑0.3 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse TGF-beta 2 protein
Met1-Ser414
Accession #
N-terminal Sequence
Ala303
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Tgfb2
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
10 kDa, reducing conditions
Publications
Read Publications using
7346-B2 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TGF-beta 2 Protein

  • BSC-1 cell growth inhibitor
  • cetermin
  • Glioblastoma-derived T-cell suppressor factor
  • G-TSF
  • MGC116892
  • polyergin
  • TGFB2
  • TGFbeta 2
  • TGF-beta 2
  • TGF-beta2
  • TGF-beta-2
  • transforming growth factor beta-2
  • transforming growth factor, beta 2

Background

TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta  superfamily that share a characteristic cysteine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems (2). Mouse TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 283 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 2 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 2 complex (8-10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature mouse TGF-beta 2 shares 100% aa identity with rat TGF-beta 2, and 97% aa identity with human, porcine, canine, equine and bovine
TGF‑ beta 2. It demonstrates cross-species activity (1). In most cells, TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta  RIII) and a type II ser/thr kinase receptor termed TGF-beta  RII, which then phosphorylates and activates another ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). In bone -related cells, however, TGF-beta 2 also signals through TGF-beta RIIB (a splice variant of TGF-beta RII), independently of TGF-beta  RIII (13). Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts (11).

  1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  2. Dunker, N. and K. Krieglstein, 2000, Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. deMartin, R. et al. (1987) EMBO J. 6:3673.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
  13. Rotzer, D. et al., (2001) EMBO J. 20:480.

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Recombinant Mouse
TGF-beta 2 Protein
7346-B2
Recombinant Mouse TGF-beta 2 Protein
Species: Mu
Applications: Bioactivity

Publications for TGF-beta 2 (7346-B2)(27)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 5 applications: Bioassay, Cell Culture, Ex Vivo, In Vivo, Tissue Culture.


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Bioassay
(20)
Cell Culture
(1)
Ex Vivo
(1)
In Vivo
(3)
Tissue Culture
(1)
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Human
(1)
Mouse
(26)
Rat
(2)
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(1)
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Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using 7346-B2 Applications Species
Larson, KC;Martens, LH;Marconi, M;Dejesus, C;Bruhn, S;Miller, TA;Tate, B;Levenson, JM; Preclinical translational platform of neuroinflammatory disease biology relevant to neurodegenerative disease Journal of neuroinflammation 2024-01-31 [PMID: 38297405] (Bioassay, Mouse) Bioassay Mouse
Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ; Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration Cell reports 2023-06-16 [PMID: 37330908] (Mouse) Mouse
Jang, HY;Kim, SJ;Park, KS;Kim, JH; Klotho prevents transforming growth factor-?2-induced senescent-like morphological changes in the retinal pigment epithelium Cell death & disease 2023-05-20 [PMID: 37210384] (In Vivo, Mouse) In Vivo Mouse
S Ma, Q Yang, N Chen, A Zheng, N Abbasi, G Wang, PR Patel, BS Cho, BA Yee, L Zhang, H Chu, SM Evans, GW Yeo, Y Zheng, WJM Huang RNA binding protein DDX5 restricts RORgammat+ Treg suppressor function to promote intestine inflammation Science Advances, 2023-02-01;9(5):eadd6165. 2023-02-01 [PMID: 36724232] (Bioassay, Mouse) Bioassay Mouse
L Xiong, Y Sun, J Huang, P Ma, X Wang, J Wang, B Chen, J Chen, M Huang, S Huang, Y Liu Long Non-Coding RNA H19 Prevents Lens Fibrosis through Maintaining Lens Epithelial Cell Phenotypes Cells, 2022-08-17;11(16):. 2022-08-17 [PMID: 36010635] (Cell Culture, Mouse) Cell Culture Mouse
M Llorián-Sa, EM Byrne, M Szczepan, K Little, M Chen, H Xu Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells Journal of Neuroinflammation, 2022-07-14;19(1):182. 2022-07-14 [PMID: 35831910] (Bioassay, Mouse) Bioassay Mouse
XJ Li, C Morgan, LA Goff, A Doetzlhofe Follistatin promotes LIN28B-mediated supporting cell reprogramming and hair cell regeneration in the murine cochlea Science Advances, 2022-02-11;8(6):eabj7651. 2022-02-11 [PMID: 35148175] (Bioassay, Mouse) Bioassay Mouse
A Sagner, I Zhang, T Watson, J Lazaro, M Melchionda, J Briscoe A shared transcriptional code orchestrates temporal patterning of the central nervous system PloS Biology, 2021-11-12;19(11):e3001450. 2021-11-12 [PMID: 34767545] (Bioassay, Mouse) Bioassay Mouse
V Mehta, KL Pang, CS Givens, Z Chen, J Huang, DT Sweet, H Jo, JS Reader, E Tzima Mechanical forces regulate endothelial-to-mesenchymal transition and atherosclerosis via an Alk5-Shc mechanotransduction pathway Science Advances, 2021-07-09;7(28):. 2021-07-09 [PMID: 34244146] (Bioassay, Mouse) Bioassay Mouse
SM Farooq, HM Ashour Type II Collagen-Specific B Cells Induce Immune Tolerance in Th1-Skewed, Th2-Skewed, and Arthritis-Prone Strains of Mice Cells, 2021-04-12;10(4):. 2021-04-12 [PMID: 33921248] (Bioassay, Mouse) Bioassay Mouse
Show All 27 Publications.

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Bioinformatics

Gene Symbol Tgfb2
Uniprot