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Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF

 (81 Publications)
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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to rmICAM/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), approximately 65-95% will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse ICAM-1/CD54 protein
Mouse ICAM-1
(Gln28-Asn485)
Accession # Q3U8M7		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Gln28
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Icam1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
76.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
110-130 kDa, reducing conditions
Publications
Read Publications using
796-IC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 0.4 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.

  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

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Publications for ICAM-1/CD54 (796-IC)(81)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 7 applications: Adhesion Assay, Binding Assay, Bioassay, Cell Culture, Control, Flow Cytometry, Surface Plasmon Resonance.


Filter By Application
Adhesion Assay
(1)
Binding Assay
(1)
Bioassay
(73)
Cell Culture
(2)
Control
(1)
Flow Cytometry
(2)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(77)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 81. Show All 81 Publications.
Publications using 796-IC Applications Species
Pruenster, M;Immler, R;Roth, J;Kuchler, T;Bromberger, T;Napoli, M;Nussbaumer, K;Rohwedder, I;Wackerbarth, LM;Piantoni, C;Hennis, K;Fink, D;Kallabis, S;Schroll, T;Masgrau-Alsina, S;Budke, A;Liu, W;Vestweber, D;Wahl-Schott, C;Roth, J;Meissner, F;Moser, M;Vogl, T;Hornung, V;Broz, P;Sperandio, M; E-selectin-mediated rapid NLRP3 inflammasome activation regulates S100A8/S100A9 release from neutrophils via transient gasdermin D pore formation Nature immunology 2023-10-30 [PMID: 37903858] (Bioassay, Transgenic Mouse, Mouse) Bioassay Transgenic Mouse, Mouse
CP Camargo, AK Muhuri, Y Alapan, LF Sestito, M Khosla, MP Manspeaker, AS Smith, CM Paulos, SN Thomas A dhesion analysis via a tumor vasculature-like microfluidic device identifies CD8+ T�cells with enhanced tumor homing to improve cell therapy Cell Reports, 2023-02-26;42(3):112175. 2023-02-26 [PMID: 36848287] (Bioassay, Mouse) Bioassay Mouse
J Li, Z Yao, X Liu, R Duan, X Yi, A Ayoub, JO Sanders, A Mesfin, L Xing, BF Boyce TGFbeta1+CCR5+ neutrophil subset increases in bone marrow and causes age-related osteoporosis in male mice Nature Communications, 2023-01-11;14(1):159. 2023-01-11 [PMID: 36631487] (Bioassay, Mouse) Bioassay Mouse
S Kohara, K Ogawa Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells Biomedicines, 2022-12-12;10(12):. 2022-12-12 [PMID: 36551990] (Bioassay, Mouse) Bioassay Mouse
S Silva Pere, M De Niz, K Serre, M Ouarné, JE Coelho, CA Franco, LM Figueiredo Immunopathology and Trypanosoma congolense parasite sequestration cause acute cerebral trypanosomiasis Elife, 2022-07-05;11(0):. 2022-07-05 [PMID: 35787830] (Bioassay, Mouse) Bioassay Mouse
L Wen, A Marki, Z Wang, M Orecchioni, J Makings, M Billitti, E Wang, SSA Suthahar, K Kim, WB Kiosses, Z Mikulski, K Ley A humanized beta2 integrin knockin mouse reveals localized intra- and extravascular neutrophil integrin activation in�vivo Cell Reports, 2022-05-31;39(9):110876. 2022-05-31 [PMID: 35649374] (Bioassay, Mouse) Bioassay Mouse
T Bromberger, S Klapproth, M Sperandio, M Moser Humanized beta2 Integrin-Expressing Hoxb8 Cells Serve as Model to Study Integrin Activation Cells, 2022-05-03;11(9):. 2022-05-03 [PMID: 35563841] (Bioassay, Mouse) Bioassay Mouse
Z Ou, E Dolmatova, R Mandavilli, H Qu, G Gafford, T White, A Valdivia, B Lassègue, MS Hernandes, KK Griendling Myeloid Poldip2 Contributes to the Development of Pulmonary Inflammation by Regulating Neutrophil Adhesion in a Murine Model of Acute Respiratory Distress Syndrome Journal of the American Heart Association, 2022-05-10;11(10):e025181. 2022-05-10 [PMID: 35535614] (Bioassay, Mouse) Bioassay Mouse
Z Jing, MJ McCarron, ML Dustin, DR Fooksman Germinal center expansion but not plasmablast differentiation is proportional to peptide-MHCII density via CD40-CD40L signaling strength Cell Reports, 2022-05-03;39(5):110763. 2022-05-03 [PMID: 35508132] (Bioassay, Mouse) Bioassay Mouse
Z Benet, Z Jing, DR Fooksman Plasma cell dynamics in the bone marrow niche Cell Reports, 2021-02-09;34(6):108733. 2021-02-09 [PMID: 33567286] (Bioassay, Mouse) Bioassay Mouse
Show All 81 Publications.

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ICAM-1/CD54 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in Leydig cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Bioinformatics

Gene Symbol Icam1
Uniprot