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Recombinant Mouse EDA-A1/Ectodysplasin A1 Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

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Recombinant Mouse EDA-A1/Ectodysplasin A1 Protein, CF Summary

Details of Functionality
Measured by its ability to compete with biotinylated rhEDA-A1 for binding to immobilized rhEDAR/Fc Chimera.
Source
Mouse myeloma cell line, NS0-derived mouse EDA-A1/Ectodysplasin A1 protein
Ala179-Ser391, with an N-terminal 6-His tag
Accession #
N-terminal Sequence
His
Structure / Form
Homotrimer
Protein/Peptide Type
Recombinant Proteins
Gene
Eda
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
27-37 kDa, reducing conditions
Publications
Read Publications using
191-ED/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse EDA-A1/Ectodysplasin A1 Protein, CF

  • ECTD1;Ectodysplasin-A;ED1;ED1-A1;ED1-A2;EDA1;EDA2;EDA-A1;EDA-A2;HED;HED1;ODT1;STHAGX1;TNLG7C;XHED;XLHED
  • EDAA1
  • EDA-A1

Background

Ectodysplasin is a 45 kDa type II transmembrane TNF superfamily protein that is associated with X-linked hypohidrotic ectodermal dysplasia (HED), a disorder of hair, tooth, and sweat gland development (1 - 4). The mouse EDA-A1 cDNA encodes a 41 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 329 extracellular region that contains a terminal TNF homology domain, a collagenous domain, and a stalk region (5 - 8). Within the collagenous and TNF homology domains, mouse EDA-A1 shares greater than 97% aa  sequence identity with bovine, canine, human, and rat EDA-A1. Multiple alternately spliced EDA variants have been described (4, 9). The dominant variant, EDA-A2, has a deletion of two amino acids that changes the receptor binding selectivity from EDAR to XEDAR (4, 9, 10). The collagenous domain of EDA-A1 mediates noncovalent homotrimer formation (7, 8). Shedding of the collagenous and TNF homology domains of EDA-A1 is accomplished by a furin-like protease. The released fragment maintains its trimeric state and ability to bind EDAR (11, 12). Some EDA-A1 polymorphisms found in HED patients alter the protease recognition site and prevent shedding (11). EDA-A1 is expressed in developing hair follicles, epidermis, teeth, sweat glands, salivary glands, and forebrain (8, 10, 13 - 15). It regulates ectodermal appendage formation and is critical to the patterning and morphogenesis of hair follicles, partially through the induction of Lymphotoxin beta (7, 14, 16). Receptor and ligand expression are regulated by factors involved in many aspects of tissue morphgenesis. EDA-A1 expression is induced by Wnt6, (14, 15) while the expression of EDAR is induced by Activin  beta A and inhibited by BMP-2, -4, and -7 (15, 17).

  1. Mikkola, M.L. and I. Thesleff (2003) Cytokine Growth Factor Rev. 14:211.
  2. Botchkarev, V.A. and M.Y. Fessing (2005) J. Invest. Dermatol. 10:247.
  3. Kere, J. et al. (1996) Nat. Genet. 13:409.
  4. Bayes, M. et al. (1998) Hum. Mol. Genet. 7:1661.
  5. Ferguson, B.M. et al. (1997) Hum. Mol. Genet. 6:1589.
  6. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. 94:13069.
  7. Ezer, S. et al. (1999) Hum. Mol. Genet. 8:2079.
  8. Mikkola, M.L. et al. (1999) Mech. Dev. 88:133.
  9. Hashimoto, T. et al. (2006) Gene 371:42.
  10. Yan, M. et al. (2000) Science 290:523.
  11. Chen, Y. et al. (2001) Proc. Natl. Acad. Sci. 98:7218.
  12. Elomaa, O. et al. (2001) Hum. Mol. Genet. 10:953.
  13. Pispa, J. et al. (2003) Gene Exp. Patterns 3:675.
  14. Laurikkala, J. et al. (2002) Development 129:2541.
  15. Laurikkala, J. et al. (2001) Dev. Biol. 229:443.
  16. Cui, C.Y. et al. (2006) Proc. Natl. Acad. Sci. 103:9142.
  17. Mou, C. et al. (2006) Proc. Natl. Acad. Sci. 103:9075.

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191-ED/CF
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Applications: Binding Activity

Publications for EDA-A1/Ectodysplasin A1 (191-ED/CF)(2)

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Bioinformatics

Gene Symbol Eda
Uniprot