Recombinant Mouse Chondroadherin His-tag Protein, CF Summary
Details of Functionality |
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.08-048 μg/mL |
Source |
Mouse myeloma cell line, NS0-derived mouse Chondroadherin protein Ala21-Thr349, with a C-terminal 6-His tag |
Accession # |
|
N-terminal Sequence |
No results obtained. Protein identity confirmed via Mass Spectrometry. |
Protein/Peptide Type |
Recombinant Proteins |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
38 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
33-38 kDa, reducing conditions
|
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, ≤ -20 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, ≤ -20 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions |
Reconstitute at 500 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse Chondroadherin His-tag Protein, CF
Background
Chondroadherin
(CHAD), also known as SLRR4A, is a 38 kDa secreted protein belonging to the small
leucine-rich proteoglycans (SLRPs) that help regulate the assembly and function
of the extracellular matrix (ECM) (1). CHAD is highly expressed
in cartilaginous tissues, with lower expression levels found in bone, tendon,
and eye (2-4). Mature mouse CHAD is a 338 amino acid (aa) protein that contains twelve
leucine-rich repeats (LRRs) including ten tandem leucine-rich repeats as well
as N-terminal and C-terminal leucine-rich domains flanked by cysteine-rich
regions. CHAD interacts with collagen II
and mediates signaling between chondrocytes and the ECM by binding to the alpha 2 beta 1 integrin,
heparan sulphate, and to cell surface proteoglycans like syndecans (5-9). In
addition, CHAD also interacts with both N- and C-terminal globular domains of
type VI collagen (10). Mouse CHAD shares 95% and 98% aa sequence identity with human
and rat CHAD, respectively.
- Neame, P.J. et al. (1994) J. Biol. Chem. 269:21547.
- Larson, T. et al. (1991) J. Biol. Chem. 266:20428.
- Mizuno, M. et al. (1996) Calcif. Tissue Int. 59:163.
- Johnson, J.M. et al. (2006) Mol. Vis. 12:1057.
- Månsson, B. et al. (2001) J. Biol. Chem. 276:32883.
- Paracuellos, P. et al. (2017) Matrix Biol. 63:106.
- Batista, M. et al. (2014) Matrix Biol. 38:84.
- Camper, L. et al. (1997) J. Cell Biol. 138:1159.
- Haglund, L. et al. (2013) J. Biol. Chem. 288:995.
- Wiberg, C. et al. (2002) J. Biol. Chem. 277:49120.
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