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Clusterin/APOJ Antibody [Unconjugated]

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Clusterin was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Clusterin was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Conservation of clusterin‐dependent alpha ‐synuclein preformed fibrils (pffs) uptake from mouse to human astrocytes. (a) Representative image of hiAstrocytes (27 days of differentiation) treated with * alpha ...read more
Genetic Strategies: Extracellular clusterin limits the uptake of alpha ‐synuclein preformed fibrils (pffs) by murine primary astrocytes. (a) Cell lysates from clusterin wild‐type (WT) and knock‐out (KO) ...read more
Clusterin protein levels are increased in two different animal models of Parkinson's disease. (a) Lysates from contralateral and ipsilateral ventral midbrain of mice injected with adeno‐associated virus (AAV)‐h ...read more
Conservation of clusterin‐dependent alpha ‐synuclein preformed fibrils (pffs) uptake from mouse to human astrocytes. (a) Representative image of hiAstrocytes (27 days of differentiation) treated with * alpha ...read more
Genetic Strategies: Extracellular clusterin limits the uptake of alpha ‐synuclein preformed fibrils (pffs) by murine primary astrocytes. (a) Cell lysates from clusterin wild‐type (WT) and knock‐out (KO) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Conjugate Size Price
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Clusterin/APOJ Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Clusterin
Glu22-Glu448
Accession # Q06890
Specificity
Detects mouse Clusterin in ELISAs and Western blots. In sandwich ELISAs, approximately 5% cross-reactivity with recombinant human Clusterin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Clu
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse Clusterin Antibody (Catalog # AF2747)
ELISA Detection: Mouse Clusterin Biotinylated Antibody (Catalog # BAF2747)
Standard: Recombinant Mouse Clusterin (Catalog # 2747-HS)
Publications
Read Publications using
AF2747 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Clusterin/APOJ Antibody [Unconjugated]

  • 40
  • 40, sulfated glycoprotein 2
  • Aging-associated gene 4 protein
  • aging-associated protein 4
  • APOJ
  • apo-J
  • Apolipoprotein J
  • CLI
  • CLIclusterin (complement lysis inhibitor, SP-40
  • CLU
  • Clusterin
  • Complement cytolysis inhibitor
  • complement lysis inhibitor
  • Complement-associated protein SP-40
  • Ku70-binding protein 1
  • KUB1SGP2
  • MGC24903
  • NA1/NA2
  • SGP-2
  • SP-40
  • sulfated glycoprotein 2
  • Testosterone-repressed prostate message 2
  • testosterone-repressed prostate message 2, apolipoprotein J)
  • TRPM-2
  • TRPM-2TRPM2

Background

Clusterin, also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40,40, is a secreted multifunctional protein that was named for its ability to induce cellular clustering. It binds a wide range of molecules and may function as a chaperone of misfolded extracellular proteins. It also participates in the control of cell proliferation, apoptosis, and carcinogenesis (1, 2). Clusterin is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart, and brain and in many epithelial tissues during embryonic development (3). Mouse Clusterin is synthesized as a precursor that contains two coiled coil domains, two nuclear localization signals (NLS), and one heparin binding domain (3-6). Intracellular cleavages of the precursor remove the signal peptide and generate comparably sized alpha and beta chains which are secreted as an 80 kDa N-glycosylated disulfide-linked heterodimer (7, 8). Mature mouse Clusterin shares 77% and 93% amino acid sequence identity with human and rat Clusterin, respectively. High μg/mL concentrations of Clusterin circulate predominantly as a component of high density lipoprotein particles, and these are internalized and degraded through interactions with LRP-2/Megalin (9, 10). In human, an alternately spliced 50 kDa isoform of Clusterin (nCLU) lacks the signal peptide and remains intracellular (5, 11). This molecule is neither glycosylated nor cleaved into alpha and beta chains (11). In the cytoplasm, nCLU destabilizes the actin cytoskeleton and inhibits NF kappa B activation (12, 13). Cellular exposure to ionizing radiation promotes the translocation of nCLU to the nucleus where it interacts with Ku70 and promotes apoptosis (5, 11). This function contrasts with the cytoprotective effect of secreted Clusterin (14). During colon cancer tumor progression there is a downregulation of the intracellular form and an upregulation of the glycosylated secreted form (11).

  1. Carver, J.A. et al. (2003) IUBMB Life 55:661.
  2. Shannan, B. et al. (2006) Cell Death Differ. 13:12.
  3. French, L.E. et al. (1993) J. Cell Biol. 122:1119.
  4. Lee, K.H. et al. (1993) Biochem. Biophys. Res. Commun. 194:1175.
  5. Leskov, K.S. et al. (2003) J. Biol. Chem. 278:11590.
  6. Pankhurst, G.J. et al. (1998) Biochemistry 37:4823.
  7. Burkey, B.F. et al. (1991) J. Lipid. Res. 32:1039.  
  8. de Silva, H.V. et al. (1990) J. Biol. Chem. 265:14292.
  9. Jenne, D.E. et al. (1991) J. Biol. Chem. 266:11030.
  10. Kounnas, M.Z. et al. (1995) J. Biol. Chem. 270:13070.
  11. Pucci, S. et al. (2004) Oncogene 23:2298.
  12. Moretti, R. M. et al. (2007) Cancer Res. 67:10325.
  13. Santilli, G. et al. (2003) J. Biol. Chem. 278:38214.
  14. Trougakos, I.P. et al. (2004) Cancer Res. 64:1834.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Clusterin/APOJ Antibody (AF2747)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA Development, ICC, ICC/IF, IHC, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
ICC
(1)
ICC/IF
(1)
IHC
(4)
Neutralization
(1)
Western Blot
(2)
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Human
(1)
Mouse
(6)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF2747 Applications Species
Shingo Ito, Ryotaro Yagi, Seiryo Ogata, Takeshi Masuda, Takashi Saito, Takaomi Saido et al. Proteomic alterations in the brain and blood-brain barrier during brain A beta accumulation in an APP knock-in mouse model of Alzheimer's disease Fluids Barriers CNS 2023-09-14 [PMID: 37705104]
JA Wei, L Liu, X Song, B Lin, J Cui, L Luo, Y Liu, S Li, X Li, KF So, S Yan, L Zhang Physical exercise modulates the microglial complement pathway in mice to relieve cortical circuitry deficits induced by mutant human TDP-43 Cell Reports, 2023-03-15;42(3):112240. 2023-03-15 [PMID: 36924491] (IHC, Neutralization, Mouse) IHC, Neutralization Mouse
A Filippini, V Salvi, V Dattilo, C Magri, S Castrezzat, R Veerhuis, D Bosisio, M Gennarelli, I Russo LRRK2 Kinase Inhibition Attenuates Astrocytic Activation in Response to Amyloid beta1-42 Fibrils Biomolecules, 2023-02-06;13(2):. 2023-02-06 [PMID: 36830676] (Western Blot, Mouse) Western Blot Mouse
O'Shea TM, Ao Y, Wang S et al. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice Nature Communications 2022-09-28 [PMID: 36171203]
HW Cheng, U Mörbe, M Lütge, C Engetschwi, L Onder, M Novkovic, C Gil-Cruz, C Perez-Shib, T Hehlgans, E Scandella, B Ludewig Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function Nature Communications, 2022-04-19;13(1):2027. 2022-04-19 [PMID: 35440118] (IHC, Mouse) IHC Mouse
Hunt LC, Graca FA, Pagala V Et al. Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy Cell reports 11/9/2021 [PMID: 34758314]
DM Go, SH Lee, SH Lee, SH Woo, K Kim, K Kim, KS Park, JH Park, SJ Ha, WH Kim, JH Choi, DY Kim Regulation of Helicobacter-induced gastritis by programmed death ligand 1-expressing classical dendritic cells Cellular and Molecular Gastroenterology and Hepatology, 2021-04-21;0(0):. 2021-04-21 [PMID: 33894424] (ICC, IHC, Mouse) ICC, IHC Mouse
Filippini A, Mutti V, Faustini G et al. Extracellular clusterin limits the uptake of alpha-synuclein fibrils by murine and human astrocytes Glia 10/12/2020 [PMID: 33045109]
K Murata, U Jadhav, S Madha, J van Es, J Dean, A Cavazza, K Wucherpfen, F Michor, H Clevers, RA Shivdasani Ascl2-Dependent Cell Dedifferentiation Drives Regeneration of Ablated Intestinal Stem Cells Cell Stem Cell, 2020-02-20;0(0):. 2020-02-20 [PMID: 32084390] (ICC/IF, Mouse) ICC/IF Mouse
Burns JC, Kelly MC, Hoa M et al. Single-cell RNA-Seq resolves cellular complexity in sensory organs from the neonatal inner ear. Nat Commun 10/15/2015 [PMID: 26469390]
Show All 13 Publications.

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Bioinformatics

Gene Symbol Clu
Uniprot