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Biglycan Antibody

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Biglycan was detected in immersion fixed paraffin-embedded sections of human kidney array using Human Biglycan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2667) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
LDL binding to the vessel wall in vitro following treatment with Site B peptide or enzymatic digestion of proteoglycan GAG chains. Tissue sections of carotid arteries from wild‐type mice with intimal hyperplasia were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Biglycan Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Biglycan
Asp38-Lys368
Accession # P21810
Specificity
Detects human Biglycan in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BGN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2667 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Biglycan Antibody

  • BGN
  • Biglycan
  • Bone/cartilage proteoglycan I
  • bone/cartilage proteoglycan-I
  • dermatan sulphate proteoglycan I
  • DSPG1
  • PGI
  • PG-S1
  • SLRR1A
  • SLRR1Abiglycan proteoglycan
  • small leucine-rich protein 1A

Background

Biglycan is a small secreted proteoglycan belonging to the leucine-rich repeat (LRR) protein family. It contains a central 12 LRR repeat domain flanked by small cysteine clusters at either side. Two glycosaminoglycans (GAG) chains are attached near the amino terminus. Biglycan binds BMP-4 and Chordin to regulate BMP-4 signaling. It may also be involved in collagen fiber assembly. Human Biglycan shares 98% amino acid sequence homology with mouse Biglycan, 97% sequence homology with rat and bovine Biglycan and 96% sequence homology with canine and equine Biglycan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Biglycan Antibody (AF2667)(17)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 6 applications: ChIP, IHC, IHC-Fr, IHC-P, Immunocytochemistry/ Immunofluorescence, Western Blot.


Filter By Application
ChIP
(1)
IHC
(1)
IHC-Fr
(2)
IHC-P
(1)
Immunocytochemistry/ Immunofluorescence
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(6)
Mouse
(3)
N/A
(1)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF2667 Applications Species
Kwack, MH;Hamida, OB;Kim, MK;Kim, MK;Sung, YK; Establishment and characterization of matched immortalized human frontal and occipital scalp dermal papilla cell lines from androgenetic alopecia Scientific reports 2023-12-05 [PMID: 38049592] (Immunocytochemistry/ Immunofluorescence, Human) Immunocytochemistry/ Immunofluorescence Human
LT Hsieh, BS Hall, J Newcombe, TA Mendum, Y Umrania, MJ Deery, WQ Shi, FJ Salguero, RE Simmonds Mycolactone causes catastrophic Sec61-dependent loss of the endothelial glycocalyx and basement membrane: a new indirect mechanism driving tissue necrosis in Mycobacterium ulcerans infection bioRxiv : the preprint server for biology, 2023-02-21;0(0):. 2/21/2023 [PMID: 36865118]
A Minns, Y Qi, K Yamamoto, K Lee, J Ahnström, S Santamaria The C-terminal domains of ADAMTS1 contain exosites involved in its proteoglycanase activity The Journal of Biological Chemistry, 2023-02-21;0(0):103048. 2023-02-21 [PMID: 36813235] (Western Blot, N/A) Western Blot N/A
Daniella dos Santos Courrol, Cristiane Castilho Fernandes da da Silva, Luan Gavião Prado, Rosa Maria Chura-Chambi, Ligia Morganti, Gisele Oliveira de Souza et al. Leptolysin, a Leptospira secreted metalloprotease of the pappalysin family with broad-spectrum activity Frontiers in Cellular and Infection Microbiology 8/23/2022 [PMID: 36081769]
S Santamaria, DR Martin, X Dong, K Yamamoto, SS Apte, J Ahnström Post-Translational Regulation And Proteolytic Activity Of The Metalloproteinase Adamts8 The Journal of Biological Chemistry, 2021-10-21;0(0):101323. 2021-10-21 [PMID: 34687701] (Western Blot, Human) Western Blot Human
Aysha Ferdoushi, Xiang Li, Nathan Griffin, Sam Faulkner, M. Fairuz B. Jamaluddin, Fangfang Gao et al. Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic Analysis Frontiers in Oncology 8/25/2020 [PMID: 32984024]
AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases Sci Rep, 2020-07-31;10(1):12912. 2020-07-31 [PMID: 32737331] (Western Blot, Mouse) Western Blot Mouse
S Kijani, AM Vázquez, M Levin, J Borén, P Fogelstran Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis Physiol Rep, 2017-07-01;5(14):. 2017-07-01 [PMID: 28716818] (IHC, Mouse) IHC Mouse
Josephina A.N. Meester, Geert Vandeweyer, Isabel Pintelon, Martin Lammens, Lana Van Hoorick, Simon De Belder et al. Loss-of-function mutations in the X-linked biglycan gene cause a severe syndromic form of thoracic aortic aneurysms and dissections Genetics in Medicine 4/1/2017 [PMID: 27632686]
Sayan Basu, Andrew J. Hertsenberg, Martha L. Funderburgh, Michael K. Burrow, Mary M. Mann, Yiqin Du et al. Human limbal biopsy–derived stromal stem cells prevent corneal scarring Science Translational Medicine 12/10/2014 [PMID: 25504883]
Show All 17 Publications.

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Bioinformatics

Gene Symbol BGN
Uniprot