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IVD Antibody (OTI1B10)

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Western Blot: IVD Antibody (1B10) [NBP2-03047] Analysis of extracts (35ug) from 9 different cell lines by using anti-IVD monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: IVD Antibody (1B10) [NBP2-03047] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IVD.
Immunohistochemistry-Paraffin: IVD Antibody (1B10) [NBP2-03047] - Staining of paraffin-embedded Human lymph node tissue using anti-IVD mouse monoclonal antibody.
Western Blot: IVD Antibody (1B10) [NBP2-03047] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IVD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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IVD Antibody (OTI1B10) Summary

Immunogen
Full length human recombinant protein of human IVD (NP_002612) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
IVD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500
Theoretical MW
46.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IVD Antibody (OTI1B10)

  • ACAD2
  • EC 1.3.99
  • EC 1.3.99.10
  • FLJ12715
  • FLJ34849
  • isovaleryl-CoA dehydrogenase
  • mitochondrial

Background

Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IVD Antibody (NBP2-03047) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IVD
Entrez