Recombinant Mouse Active Cathepsin C/DPPI Protein, CF

• 6 months from date of receipt, -20 to -70 °C as supplied.
• 3 months, -20 to -70 °C under sterile conditions after opening.

• Assay Buffer: 50 mM MES, 50 mM NaCl, 5 mM DTT, pH 5.5
• Recombinant Mouse Active Cathepsin C/DPPI (rmCathepsin C) (Catalog # 2336-CY)
• Fluorogenic Peptide Substrate: Gly-Arg-AMC (Bachem, Catalog # I-1215), 10 mM stock in DMSO
• F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
• Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent

1. Dilute rmCathepsin C to 0.01 ng/µL in Assay Buffer.
2. Dilute Substrate to 800 µM in Assay Buffer.
3. Load into a black well plate 50 µL of 0.01 ng/µL rmCathepsin C, and start the reaction by adding 50 µL of 800 µM Substrate. Include a Substrate Blank containing Assay Buffer in place of rmCathepsin C.
4. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
5. Calculate specific activity:

     *Adjusted for Substrate Blank

     **Derived using calibration standard 7-Amino, 4-Methyl Coumarin (AMC) (Sigma, Catalog # A-9891).

• rmCathepsin C: 0.0005 µg
• Substrate: 400 µM