Cathepsin B Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Cathepsin B is the first described member of the family of lysosomal cysteine proteases (1). Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins, including pro and active caspases, prorenin and secretory leucoprotease inhibitor (SLPI) (2‑4). Therefore, Cathepsin B may play a role in activation and inactivation of caspases, activation of renin and inactivation of SLPI, the key steps in apoptosis, angiotensin production, and progression of emphysema, respectively. Because of its increased levels and redistribution in human and animal tumors, Cathepsin B may also have a role in invasion and metastasis (5). In addition to the lysosome, Cathepsin B can be secreted or associated with plasma membrane, cytoplasm, and nucleus. It is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme (5).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC, Western Blot.

Showing Publications 1 - 10 of 51. Show All 51 Publications. Collapse Publications.

Publications using AF965	Applications	Species
Makar, AN;Boraman, A;Mosen, P;Simpson, JE;Marques, J;Michelberger, T;Aitken, S;Wheeler, AP;Winter, D;von Kriegsheim, A;Gammoh, N; The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation Nature communications 2024-09-05 [PMID: 39231962] (Western Blot, Mouse)	Western Blot	Mouse
Takahashi, H;Bhagwagar, S;Nies, SH;Ye, H;Han, X;Chiasseu, MT;Wang, G;Mackenzie, IR;Strittmatter, SM; Reduced progranulin increases tau and ?-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase Nature communications 2024-02-16 [PMID: 38365772] (Western Blot, Mouse)	Western Blot	Mouse
Do?ru, AG;Rehders, M;Brix, K; Investigations on Primary Cilia of Nthy-ori 3-1 Cells upon Cysteine Cathepsin Inhibition or Thyrotropin Stimulation International journal of molecular sciences 2023-05-26 [PMID: 37298246] (Western Blot, Human)	Western Blot	Human
Zhang, T;Pang, W;Feng, T;Guo, J;Wu, K;Nunez Santos, M;Arthanarisami, A;Nana, AL;Nguyen, Q;Kim, PJ;Jankowsky, JL;Seeley, WW;Hu, F; TMEM106B regulates microglial proliferation and survival in response to demyelination Science advances 2023-05-05 [PMID: 37146150] (Western Blot, Mouse)	Western Blot	Mouse
Jennifer Hua, Elvira Garcia de Paco, Nathalie Linck, Tangui Maurice, Catherine Desrumaux, Bénédicte Manoury et al. Microglial P2X4 receptors promote ApoE degradation and contribute to memory deficits in Alzheimer’s disease Cellular and Molecular Life Sciences 5/5/2023 [PMID: 37145189]
Xinyu Zhu, Sanjana Surya Prakash, Geoffrey McAuliffe, Ping-Yue Pan Synaptojanin1 Modifies Endolysosomal Parameters in Cultured Ventral Midbrain Neurons eNeuro 4/18/2023 [PMID: 37072173]
Á Gaudioso, X Jiang, J Casas, EH Schuchman, MD Ledesma Sphingomyelin 16:0 is a therapeutic target for neuronal death in acid sphingomyelinase deficiency Cell Death & Disease, 2023-04-06;14(4):248. 2023-04-06 [PMID: 37024473] (ICC, Mouse)	ICC	Mouse
Y Qiu, X Shen, O Ravid, D Atrakchi, D Rand, AE Wight, HJ Kim, S Liraz-Zalt, I Cooper, M Schnaider, H Cantor Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer's disease Proceedings of the National Academy of Sciences of the United States of America, 2023-02-02;120(6):e2218915120. 2023-02-02 [PMID: 36730200] (IHC, Mouse)	IHC	Mouse
Parchure A, Tian M, Stalder D et al. Liquid-liquid phase separation facilitates the biogenesis of secretory storage granules The Journal of cell biology 12/5/2022 [PMID: 36173346]
Parchure A, Tian M, Stalder D et al. Liquid-liquid phase separation facilitates the biogenesis of secretory storage granules The Journal of cell biology 2022-12-05 [PMID: 36173346]
E Ratto, SR Chowdhury, NS Siefert, M Schneider, M Wittmann, D Helm, W Palm Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly Nature Communications, 2022-08-17;13(1):4848. 2022-08-17 [PMID: 35977928] (Western Blot, Mouse)	Western Blot	Mouse
T Feng, L Luan, II Katz, M Ullah, VM Van Deerli, JQ Trojanowsk, EB Lee, F Hu TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss Acta neuropathologica communications, 2022-03-14;10(1):33. 3/14/2022 [PMID: 35287730]
K Uehara, M Koyanagi-A, T Koide, T Itoh, T Aoi Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids Stem Cell Reports, 2022-03-03;0(0):. 2022-03-03 [PMID: 35245440] (Western Blot, Mouse)	Western Blot	Mouse
Jiandong Sun, Yan Liu, Xiaoning Hao, Weiju Lin, Wenyue Su, Emerald Chiang et al. LAMTOR1 inhibition of TRPML1‐dependent lysosomal calcium release regulates dendritic lysosome trafficking and hippocampal neuronal function The EMBO Journal 3/1/2022 [PMID: 35099830]
TT Dang, SH Back Translation Inhibitors Activate Autophagy Master Regulators TFEB and TFE3 International Journal of Molecular Sciences, 2021-11-08;22(21):. 11/8/2021 [PMID: 34769510]
Torres JLN, Shanahan SL, Chassefeyre R, Bueno SL LC3B phosphorylation regulates FYCO1 binding and directional transport of autophagosomes Curr Biol 6/19/2021 [PMID: 34146484]
Du H, Wong MY, Zhang T et al. A multifaceted role of progranulin in regulating amyloid-beta dynamics and responses Life science alliance 7/1/2021 [PMID: 34103390]
Roney JC, Li S, Farfel-Becker T et al. Lipid-mediated motor-adaptor sequestration impairs axonal lysosome delivery leading to autophagic stress and dystrophy in Niemann-Pick type C Developmental cell 4/13/2021 [PMID: 33878344]
H Iwama, S Mehanna, M Imasaka, S Hashidume, H Nishiura, KI Yamamura, C Suzuki, Y Uchiyama, E Hatano, M Ohmuraya Cathepsin B and D deficiency in the mouse pancreas induces impaired autophagy and chronic pancreatitis Scientific Reports, 2021-03-23;11(1):6596. 2021-03-23 [PMID: 33758261] (IHC, Mouse)	IHC	Mouse
Yasuhiko Bando, Nobuko Tokuda, Yudai Ogasawara, Go Onozawa, Arata Nagasaka, Koji Sakiyama et al. Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae Histochemistry and Cell Biology 4/1/2021 [PMID: 33398436]
L Bonet-Ponc, A Beilina, CD Williamson, E Lindberg, JH Kluss, S Saez-Atien, N Landeck, R Kumaran, A Mamais, CKE Bleck, Y Li, MR Cookson LRRK2 mediates tubulation and vesicle sorting from lysosomes Sci Adv, 2020-11-11;6(46):. 2020-11-11 [PMID: 33177079] (ICC, Human)	ICC	Human
M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations Acta Neuropathol Commun, 2020-10-07;8(1):163. 2020-10-07 [PMID: 33028409] (Western Blot, Mouse)	Western Blot	Mouse
Georg Werner, Markus Damme, Martin Schludi, Johannes Gnörich, Karin Wind, Katrin Fellerer et al. Loss of TMEM 106B potentiates lysosomal and FTLD ‐like pathology in progranulin‐deficient mice EMBO reports 10/5/2020 [PMID: 32929860]
Zhang J, Velmeshev D, Hashimoto K et al. Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency Nature 12/17/2020 [PMID: 32866962]
Zhang J, Velmeshev D, Hashimoto K et al. Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency Nature 2020-12-17 [PMID: 32866962]
Feng T, Mai S, Roscoe JM et al. Loss of TMEM106B and PGRN leads to severe lysosomal abnormalities and neurodegeneration in mice EMBO Rep. 8/10/2020 [PMID: 32852886]
Panos G. Ziros, Cédric O. Renaud, Dionysios V. Chartoumpekis, Massimo Bongiovanni, Ioannis G. Habeos, Xiao-Hui Liao et al. Mice Hypomorphic for Keap1, a Negative Regulator of the Nrf2 Antioxidant Response, Show Age-Dependent Diffuse Goiter with Elevated Thyrotropin Levels Thyroid 1/1/2021 [PMID: 32689903]
J Selkrig, N Li, A Hausmann, MSJ Mangan, M Zietek, A Mateus, J Bobonis, A Sueki, H Imamura, B El Debs, G Sigismondo, BI Florea, HS Overkleeft, N Kopitar-Je, B Turk, P Beltrao, MM Savitski, E Latz, WD Hardt, J Krijgsveld, A Typas Spatiotemporal proteomics uncovers cathepsin-dependent macrophage cell death during Salmonella infection Nat Microbiol, 2020-06-08;0(0):. 2020-06-08 [PMID: 32514074] (Western Blot, Mouse)	Western Blot	Mouse
Cui W, Sathyanarayan A, Lopresti M et al. Lipophagy-derived fatty acids undergo extracellular efflux via lysosomal exocytosis Autophagy 2/19/2020 [PMID: 32070194]
J Schwenck, A Maurer, B Fehrenbach, R Mehling, P Knopf, N Mucha, D Haupt, K Fuchs, CM Griessinge, D Bukala, J Holstein, M Schaller, IG Menendez, K Ghoreschi, L Quintanill, M Gütschow, S Laufer, T Reinheckel, M Röcken, H Kalbacher, BJ Pichler, M Kneilling Cysteine-type cathepsins promote the effector phase of acute cutaneous delayed-type hypersensitivity reactions Theranostics, 2019-05-31;9(13):3903-3917. 2019-05-31 [PMID: 31281521] (Flow Cytometry, Western Blot, Mouse)	Flow Cytometry, Western Blot	Mouse
T Farfel-Bec, JC Roney, XT Cheng, S Li, SR Cuddy, ZH Sheng Neuronal Soma-Derived Degradative Lysosomes Are Continuously Delivered to Distal Axons to Maintain Local Degradation Capacity Cell Rep, 2019-07-02;28(1):51-64.e4. 2019-07-02 [PMID: 31269450] (ICC, Mouse)	ICC	Mouse
Y Takagaki, S Shi, M Katoh, M Kitada, K Kanasaki, D Koya Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice Sci Rep, 2019-05-17;9(1):7519. 5/17/2019 [PMID: 31101909]
Enrique Gabandé‐Rodríguez, Azucena Pérez‐Cañamás, Beatriz Soto‐Huelin, Daniel N Mitroi, Sara Sánchez‐Redondo, Elena Martínez‐Sáez et al. Lipid‐induced lysosomal damage after demyelination corrupts microglia protective function in lysosomal storage disorders The EMBO Journal 1/15/2019 [PMID: 30530526]
JK Götzl, AV Colombo, K Fellerer, A Reifschnei, G Werner, S Tahirovic, C Haass, A Capell Early lysosomal maturation deficits in microglia triggers enhanced lysosomal activity in other brain cells of progranulin knockout mice Mol Neurodegener, 2018-09-04;13(1):48. 2018-09-04 [PMID: 30180904] (Western Blot, Mouse)	Western Blot	Mouse
Chan Choo Yap, Laura Digilio, Lloyd P. McMahon, A. Denise R. Garcia, Bettina Winckler Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes Journal of Cell Biology 9/3/2018 [PMID: 29907658]
XT Cheng, YX Xie, B Zhou, N Huang, T Farfel-Bec, ZH Sheng Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons J. Cell Biol., 2018-04-25;0(0):. 2018-04-25 [PMID: 29695488] (Western Blot, Rat)	Western Blot	Rat
Rakshamani Tripathi, Leann S. Fiore, Dana L. Richards, Yuchen Yang, Jinpeng Liu, Chi Wang et al. Abl and Arg mediate cysteine cathepsin secretion to facilitate melanoma invasion and metastasis Science Signaling 2/22/2018 [PMID: 29463776]
B Lloyd-Lewi, CC Krueger, TJ Sargeant, ME D'Angelo, MJ Deery, R Feret, JA Howard, KS Lilley, CJ Watson Stat3 mediated alterations in lysosomal membrane protein composition J. Biol. Chem., 2018-01-17;0(0):. 1/17/2018 [PMID: 29343516]
Zoe A. Klein, Hideyuki Takahashi, Mengxiao Ma, Massimiliano Stagi, Melissa Zhou, TuKiet T. Lam et al. Loss of TMEM106B Ameliorates Lysosomal and Frontotemporal Dementia-Related Phenotypes in Progranulin-Deficient Mice Neuron 7/19/2017 [PMID: 28728022]
K Takahashi, H Mashima, K Miura, D Maeda, A Goto, T Goto, GH Sun-Wada, Y Wada, H Ohnishi Disruption of Small GTPase Rab7 Exacerbates the Severity of Acute Pancreatitis in Experimental Mouse Models Sci Rep, 2017-06-06;7(1):2817. 2017-06-06 [PMID: 28588238] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
Yasuhiko Bando, Miyuki Yamamoto, Koji Sakiyama, Hide Sakashita, Fuyoko Taira, Genki Miyake et al. Retinoic acid regulates cell-shape and -death of E-FABP (FABP5)-immunoreactive septoclasts in the growth plate cartilage of mice Histochemistry and Cell Biology 9/1/2017 [PMID: 28500502]
Cathepsin B modulates lysosomal biogenesis and host defense against Francisella novicida infection J Exp Med, 2016-08-22;0(0):. 8/22/2016 [PMID: 27551156]
Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer. PLoS ONE, 2015-04-30;10(4):e0120348. 2015-04-30 [PMID: 25927437] (IHC, Mouse)	IHC	Mouse
Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells. Mol Cancer, 2015-02-15;14(0):39. 2015-02-15 [PMID: 25744631] (Western Blot, Mouse)	Western Blot	Mouse
Lee , Hwa-Youn, Lee , Geum-Hwa, Lee , Mi-Rin, Kim , Hye-Kyun, Kim , Nan-youn, Kim , Seung-Hy, Lee , Yong-Chu, Kim , Hyung-Ry, Chae , Han-Jung Eucommia ulmoides Oliver extract, aucubin, and geniposide enhance lysosomal activity to regulate ER stress and hepatic lipid accumulation. PLoS ONE, 2013-12-11;8(12):e81349. 2013-12-11 [PMID: 24349058] (ICC, Rat)	ICC	Rat
Shin , Jin Na, Fattah , Elmoataz, Bhattacharya , Abhisek, Ko , Soyoung, Eissa , N Tony Inflammasome activation by altered proteostasis. J Biol Chem, 2013-10-31;288(50):35886-95. 2013-10-31 [PMID: 24178293] (Western Blot, Mouse)	Western Blot	Mouse
Cyrill Bussy, Erwan Paineau, Julien Cambedouzou, Nathalie Brun, Claudie Mory, Barbara Fayard et al. Intracellular fate of carbon nanotubes inside murine macrophages: pH-dependent detachment of iron catalyst nanoparticles Particle and Fibre Toxicology 12/1/2013 [PMID: 23800198]
K Shchors, H Nozawa, J Xu, F Rostker, L Swigart-Brown, G Evan et al. Increased invasiveness of MMP-9-deficient tumors in two mouse models of neuroendocrine tumorigenesis Oncogene 1/24/2013 [PMID: 22391572]
Shree T, Olson OC, Elie BT Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer. Genes Dev., 2011-12-01;25(23):2465-79. 2011-12-01 [PMID: 22156207] (Western Blot, Mouse)	Western Blot	Mouse
Margot G. Paulick, Matthew Bogyo Development of Activity-Based Probes for Cathepsin X ACS Chemical Biology 6/17/2011 [PMID: 21322635]
Nielsen R, Courtoy PJ, Jacobsen C, Dom G, Lima WR, Jadot M, Willnow TE, Devuyst O, Christensen EI Endocytosis provides a major alternative pathway for lysosomal biogenesis in kidney proximal tubular cells. Proc. Natl. Acad. Sci. U.S.A., 2007-03-16;104(13):5407-12. 2007-03-16 [PMID: 17369355] (Western Blot, Mouse)	Western Blot	Mouse
Show All 51 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	IHC-P	Mouse	12/19/2014	View Summary Application Immunohistochemistry-Paraffin Sample Tested Kidney paraffin section Species Mouse

Array AF965

• Cathepsin B Antibodies
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• Cathepsin B Lysates
• Cathepsin B Proteins
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• Cathepsin B Proteins and Enzymes