![Immunocytochemistry/Immunofluorescence: RRM2 Antibody [NBP1-31661] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RRM2 protein stained by RRM2 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/RRM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31661-img0011.jpg "Immunocytochemistry/Immunofluorescence: RRM2 Antibody [NBP1-31661] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RRM2 protein stained by RRM2 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
| Reactivity | Hu, Mu, Rt, Ze, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human RRM2. The exact sequence is proprietary. |
| Localization | Cytoplasm |
| Predicted Species | Bovine (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RRM2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IHC-P | Mouse | 08/11/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for RRM2 Antibody (NBP1-31661)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse tumor tissues were stained with RRM2 antibody at 1:50 dilution. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RRM2-Antibody-Immunohistochemistry-Paraffin-NBP1-31661-img0012.jpg "Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse tumor tissues were stained with RRM2 antibody at 1:50 dilution. Image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: RRM2 Antibody [NBP1-31661] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RRM2 protein stained by RRM2 antibody [N1C1] diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/RRM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31661-img0009.jpg "Immunocytochemistry/Immunofluorescence: RRM2 Antibody [NBP1-31661] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RRM2 protein stained by RRM2 antibody [N1C1] diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse muscle. RRM2 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RRM2-Antibody-Immunohistochemistry-Paraffin-NBP1-31661-img0006.jpg "Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse muscle. RRM2 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Rat intestine. RRM2 antibody [N1C1] iluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RRM2-Antibody-Immunohistochemistry-Paraffin-NBP1-31661-img0007.jpg "Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Rat intestine. RRM2 antibody [N1C1] iluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse muscle. RRM2 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RRM2-Antibody-Immunohistochemistry-Paraffin-NBP1-31661-img0008.jpg "Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse muscle. RRM2 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: RRM2 Antibody [NBP1-31661] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [N1C1] diluted at 1:1000.](http://images.novusbio.com/fullsize/antibody/nbp1-31661_rabbit-polyclonal-rrm2-antibody-western-blot-14320248566..jpg "Western Blot: RRM2 Antibody [NBP1-31661] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [N1C1] diluted at 1:1000.")
![Immunohistochemistry: RRM2 Antibody [NBP1-31661] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using RRM2 antibody [N1C1] (NBP1-31661) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-31661_rabbit-polyclonal-rrm2-antibody-910202420134094.jpg "Immunohistochemistry: RRM2 Antibody [NBP1-31661] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using RRM2 antibody [N1C1] (NBP1-31661) at 1:300 dilution.")
![Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. RRM2 antibody [N1C1] (NBP1-31661) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31661_rabbit-polyclonal-rrm2-antibody-910202420115026.jpg "Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. RRM2 antibody [N1C1] (NBP1-31661) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: RRM2 Antibody [NBP1-31661] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-31661_rabbit-polyclonal-rrm2-antibody-1610202419414577.jpg "Western Blot: RRM2 Antibody [NBP1-31661] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
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![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
