Recombinant Human sCD4 Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations (1). Mature human CD4 consists of a 371 amino acid (aa) extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain (2). Within the ECD, human CD4 shares approximately 52% aa sequence identity with mouse and rat CD4. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4⁺ or CD8⁺ mature T cells (3). CD4⁺ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4⁺ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (4). CD4 is reexpressed on circulating CD8⁺ T cells upon activation and contributes to their cytotoxic effector activity (5). In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain (6-9). Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse (6). CD4 binds directly to MHC class II molecules on antigen presenting cells (10). This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction (1, 11). Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck (12). CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1 (7, 13-15).

We have publications tested in 2 confirmed species: Human, Virus.

We have publications tested in 3 applications: Binding Assay, Bioassay, Surface Plasmon Resonance.

Showing Publications 1 - 6 of 6.

Publications using 514-CD	Applications	Species
Y Zheng, XW Yang, D Schols, M Mori, B Botta, A Chevigné, M Mulinge, A Steinmetz, JC Schmit, C Seguin-Dev Active Components from Cassia abbreviata Prevent HIV-1 Entry by Distinct Mechanisms of Action International Journal of Molecular Sciences, 2021-05-10;22(9):. 2021-05-10 [PMID: 34068829] (Bioassay, Human)	Bioassay	Human
D Wensel, Y Sun, Z Li, S Zhang, C Picarillo, T McDonagh, D Fabrizio, M Cockett, M Krystal, J Davis Discovery and Characterization of a Novel CD4-Binding Adnectin with Potent Anti-HIV Activity Antimicrob. Agents Chemother., 2017-07-25;0(0):. 2017-07-25 [PMID: 28584151] (Binding Assay)	Binding Assay
Jin Q, Chen H, Wang X, Zhao L, Xu Q, Wang H, Li G, Yang X, Ma H, Wu H, Ji X The Effects of the Recombinant CCR5 T4 Lysozyme Fusion Protein on HIV-1 Infection. PLoS ONE, 2015-07-08;10(7):e0131894. 2015-07-08 [PMID: 26154172] (Bioassay, Human)	Bioassay	Human
Hayasaka H, Kobayashi D, Yoshimura H, Nakayama E, Shioda T, Miyasaka M The HIV-1 Gp120/CXCR4 axis promotes CCR7 ligand-dependent CD4 T cell migration: CCR7 homo- and CCR7/CXCR4 hetero-oligomer formation as a possible mechanism for up-regulation of functional CCR7. PLoS ONE, 2015-02-17;10(2):e0117454. 2015-02-17 [PMID: 25688986] (Bioassay, Human)	Bioassay	Human
Zou Z, Chastain A, Moir S Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids. PLoS ONE, 2011-09-08;6(9):e24559. 2011-09-08 [PMID: 21931755] (Surface Plasmon Resonance, Virus)	Surface Plasmon Resonance	Virus
Ivie SE, Fennessey CM, Sheng J, Rubin DH, McClain MS Gene-trap mutagenesis identifies mammalian genes contributing to intoxication by Clostridium perfringens epsilon-toxin. PLoS ONE, 2011-03-11;6(3):e17787. 2011-03-11 [PMID: 21412435] (Binding Assay, Human)	Binding Assay	Human

Array 514-CD

• CD4 Antibodies
• CD4 Antibody Pair
• CD4 ELISA Kits
• CD4 Lysate
• CD4 Proteins
• CD4 Proteins and Enzymes