Name: Recombinant Human ICAM-1/CD54 Protein, CF
Brand: Novus Biologicals
SKU: ADP4
Price: 423 USD

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	Bioactivity
Format	Carrier-Free

Details of Functionality	Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10⁴ cells/well are added to Recombinant Human ICAM‑1/CD54 coated plates (12.5 μg/mL with 100 μL/well), >30% cells will adhere after 1 hour incubation at 37 °C. Optimal dilutions should be determined by each laboratory for each application.
Source	Mouse myeloma cell line, NS0-derived human ICAM-1/CD54 protein Met1-Glu480
Accession #	CAA30051
N-terminal Sequence	Not obtained, Predicted Gln28
Protein/Peptide Type	Recombinant Proteins
Gene	ICAM1
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note	<0.10 EU per 1 μg of the protein by the LAL method.

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS, containing Calcium and Magnesium with Sorbitol and with Trehalose.
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions	Reconstitute at 400 μg/mL in sterile PBS.

Publications using ADP4	Applications	Species
Lacouture, C;Chaves, B;Guipouy, D;Houmadi, R;Duplan-Eche, V;Allart, S;Destainville, N;Dupré, L; LFA-1 nanoclusters integrate TCR stimulation strength to tune T-cell cytotoxic activity Nature communications 2024-01-09 [PMID: 38195629] (Bioassay, Human)	Bioassay	Human
Sindram, E;Caballero-Oteyza, A;Kogata, N;Huang, S;Alizadeh, Z;Gamez-Díaz, L;Fazlollahi, MR;Peng, X;Grimbacher, B;Way, M;Proietti, M; ARPC5 deficiency leads to severe early onset systemic inflammation and mortality Disease models & mechanisms 2023-06-29 [PMID: 37382373] (Bioassay, Human)	Bioassay	Human
L Kaltenbach, P Martzloff, SK Bambach, N Aizarani, M Mihlan, A Gavrilov, KM Glaser, M Stecher, R Thünauer, A Thiriot, K Heger, K Kierdorf, S Wienert, UH von Andria, M Schmidt-Su, C Nerlov, F Klauschen, A Roers, M Bajénoff, D Grün, T Lämmermann Slow integrin-dependent migration organizes networks of tissue-resident mast cells Nature Immunology, 2023-04-20;0(0):. 2023-04-20 [PMID: 37081147] (Bioassay, Mouse)	Bioassay	Mouse
C Weichwald, I Zettl, I Ellinger, K Niespodzia, EE Waltl, S Villazala-, D Ivanov, J Eckl-Dorna, V Niederberg, R Valenta, S Flicker Antibody Conjugates Bispecific for Pollen Allergens and ICAM-1 with Potential to Prevent Epithelial Allergen Transmigration and Rhinovirus Infection International Journal of Molecular Sciences, 2023-02-01;24(3):. 2023-02-01 [PMID: 36769047] (ELISA Capture, Human, N/A)	ELISA Capture	Human, N/A
R Fitterer, T Lajqi, SA Kranig, M Braun, N Theissig, N Kuss, J Pöschl, D Frommhold, H Hudalla L-Arginine Modulates Neonatal Leukocyte Recruitment in a Gestational Age-Dependent Manner J Clin Med, 2020-08-27;9(9):. 2020-08-27 [PMID: 32867030] (Bioassay, Human)	Bioassay	Human
J Gamara, L Davis, E Rollet-Lab, T Hongu, Y Funakoshi, Y Kanaho, F Aoudjit, SG Bourgoin Assessment of Arf6 Deletion in PLB-985 Differentiated in Neutrophil-Like Cells and in Mouse Neutrophils: Impact on Adhesion and Migration Mediators Inflamm., 2020-04-07;2020(0):2713074. 2020-04-07 [PMID: 32322163] (Bioassay, Human)	Bioassay	Human
F Caccuri, A Bugatti, S Corbellini, S Roversi, A Zani, P Mazzuca, S Marsico, A Caruso, C Giagulli The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3) Int J Mol Sci, 2019-10-24;20(21):. 2019-10-24 [PMID: 31653015] (Bioassay, Human)	Bioassay	Human
NV Ayala-Nune, G Follain, F Delalande, A Hirschler, E Partiot, GL Hale, BC Bollweg, J Roels, M Chazal, F Bakoa, M Carocci, S Bourdoulou, O Faklaris, SR Zaki, A Eckly, B Uring-Lamb, F Doussau, S Cianferani, C Carapito, FMJ Jacobs, N Jouvenet, JG Goetz, R Gaudin Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells Nat Commun, 2019-09-27;10(1):4430. 2019-09-27 [PMID: 31562326] (Bioassay, Human)	Bioassay	Human
MJ Bertrand, M Abran, F Maafi, D Busseuil, N Merlet, T Mihalache-, P Geoffroy, PL Tardif, A Abulrob, M Arbabi-Gha, F Ni, M Sirois, PL L'Allier, É Rhéaume, F Lesage, JC Tardif In Vivo Near-Infrared Fluorescence Imaging of Atherosclerosis Using Local Delivery of Novel Targeted Molecular Probes Sci Rep, 2019-02-25;9(1):2670. 2019-02-25 [PMID: 30804367] (Affinity Purification, Human)	Affinity Purification	Human
D Wolf, N Anto-Miche, H Blankenbac, A Wiedemann, K Buscher, JD Hohmann, B Lim, M Bäuml, A Marki, M Mauler, D Duerschmie, Z Fan, H Winkels, D Sidler, P Diehl, DM Zajonc, I Hilgendorf, P Stachon, T Marchini, F Willecke, M Schell, B Sommer, C von Zur Mu, J Reinöhl, T Gerhardt, EF Plow, V Yakubenko, P Libby, C Bode, K Ley, K Peter, A Zirlik A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense Nat Commun, 2018-02-06;9(1):525. 2018-02-06 [PMID: 29410422] (Bioassay, Human)	Bioassay	Human
A Gil-Krzews, MB Saeed, A Oszmiana, ER Fischer, K Lagrue, WA Gahl, WJ Introne, JE Coligan, DM Davis, K Krzewski An actin cytoskeletal barrier inhibits lytic granule release from natural killer cells in patients with Chediak-Higashi syndrome J. Allergy Clin. Immunol., 2017-12-11;0(0):. 2017-12-11 [PMID: 29241728] (Bioassay, Human)	Bioassay	Human
RLG Maus, JW Jakub, WK Nevala, TA Christense, K Noble-Orcu, Z Sachs, TJ Hieken, SN Markovic Human Melanoma-Derived Extracellular Vesicles Regulate Dendritic Cell Maturation Front Immunol, 2017-03-29;8(0):358. 2017-03-29 [PMID: 28424693] (Bioassay, Human)	Bioassay	Human
J Miku?a-Pie, P Uruski, M Kuci?ska, A Tykarski, K Ksi??ek The protective activity of mesothelial cells against peritoneal growth of gastrointestinal tumors: The role of soluble ICAM-1 Int. J. Biochem. Cell Biol, 2017-03-18;86(0):26-31. 2017-03-18 [PMID: 28323210] (Bioassay, Human)	Bioassay	Human
Senescent peritoneal mesothelium creates a niche for ovarian cancer metastases Cell Death Dis, 2016-12-29;7(12):e2565. 2016-12-29 [PMID: 28032864] (Bioassay, Human)	Bioassay	Human
A Rational Engineering Strategy for Designing Protein A-Binding Camelid Single-Domain Antibodies PLoS ONE, 2016-09-15;11(9):e0163113. 2016-09-15 [PMID: 27631624] (Surface Plasmon Resonance)	Surface Plasmon Resonance
Maria Risager Jensen Structural basis for simvastatin competitive antagonism of complement receptor 3 J Biol Chem, 2016-06-23;0(0):. 2016-06-23 [PMID: 27339893] (Bioassay)	Bioassay
Lagrue K, Carisey A, Morgan D, Chopra R, Davis D Lenalidomide augments actin remodeling and lowers NK-cell activation thresholds. Blood, 2015-05-22;126(1):50-60. 2015-05-22 [PMID: 26002964] (Bioassay, Human)	Bioassay	Human
Borgman K, van Zanten T, Manzo C, Cabezon R, Cambi A, Benitez-Ribas D, Garcia-Parajo M Priming by chemokines restricts lateral mobility of the adhesion receptor LFA-1 and restores adhesion to ICAM-1 nano-aggregates on human mature dendritic cells. PLoS ONE, 2014-06-19;9(6):e99589. 2014-06-19 [PMID: 24945611] (Bioassay, Human)	Bioassay	Human
Doritchamou J, Sossou-tchatcha S, Cottrell G, Moussiliou A, Hounton Houngbeme C, Massougbodji A, Deloron P, Ndam N Dynamics in the cytoadherence phenotypes of Plasmodium falciparum infected erythrocytes isolated during pregnancy. PLoS ONE, 2014-06-06;9(6):e98577. 2014-06-06 [PMID: 24905223] (Bioassay, Human)	Bioassay	Human
Weckbach L, Gola A, Winkelmann M, Jakob S, Groesser L, Borgolte J, Pogoda F, Pick R, Pruenster M, Muller-Hocker J, Deindl E, Sperandio M, Walzog B The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via beta2 integrins (CD11/CD18). Blood, 2014-01-23;123(12):1887-96. 2014-01-23 [PMID: 24458438] (Bioassay, Human)	Bioassay	Human
Dalli , Jesmond, Norling , Lucy V, Montero-Melendez , Trinidad, Federici Canova , Donata, Lashin , Hazem, Pavlov , Anton M, Sukhorukov , Gleb B, Hinds , Charles, Perretti , Mauro Microparticle alpha-2-macroglobulin enhances pro-resolving responses and promotes survival in sepsis. EMBO Mol Med, 2013-12-15;6(1):27-42. 2013-12-15 [PMID: 24357647] (Bioassay, Human)	Bioassay	Human
Niyonsaba F, Madera L, Afacan N, Okumura K, Ogawa H, Hancock R The innate defense regulator peptides IDR-HH2, IDR-1002, and IDR-1018 modulate human neutrophil functions. J Leukoc Biol, 2013-04-24;94(1):159-70. 2013-04-24 [PMID: 23616580] (Bioassay, Human)	Bioassay	Human
Li N, Mao D, Lu S, Tong C, Zhang Y, Long M Distinct binding affinities of Mac-1 and LFA-1 in neutrophil activation. J Immunol, 2013-03-20;190(8):4371-81. 2013-03-20 [PMID: 23514737] (Bioassay, Human)	Bioassay	Human
Brown A, Dobbie I, Alakoskela J, Davis I, Davis D Super-resolution imaging of remodeled synaptic actin reveals different synergies between NK cell receptors and integrins. Blood, 2012-09-10;120(18):3729-40. 2012-09-10 [PMID: 22966166] (Bioassay, Human)	Bioassay	Human
Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, Kettritz R Complement Receptor Mac-1 Is an Adaptor for NB1 (CD177)-mediated PR3-ANCA Neutrophil Activation. J. Biol. Chem., 2010-12-30;286(9):7070-81. 2010-12-30 [PMID: 21193407] (Adhesion Assay, Human)	Adhesion Assay	Human
Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4. J. Hepatol., 2009-07-30;51(4):734-49. 2009-07-30 [PMID: 19703720] (Bioassay, Human)	Bioassay	Human
Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA (Standard))	ELISA (Standard)
Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007-10-22;329(1):214-8. 2007-10-22 [PMID: 17964594] (ELISA (Standard))	ELISA (Standard)
Brooks AM, Bates ME, Vrtis RF, Jarjour NN, Bertics PJ, Sedgwick JB Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma. Am. J. Respir. Cell Mol. Biol., 2006-05-25;35(4):503-11. 2006-05-25 [PMID: 16728704] (Bioassay, Human)	Bioassay	Human
Tamai R, Asai Y, Ogawa T Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis. Infect. Immun., 2005-10-01;73(10):6290-8. 2005-10-01 [PMID: 16177300] (ELISA (Capture), Bacteria)	ELISA (Capture)	Bacteria
Yamamoto H, Nagata M, Sakamoto Y CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1. Ann. Allergy Asthma Immunol., 2005-02-01;94(2):292-300. 2005-02-01 [PMID: 15765748] (Bioassay, Human)	Bioassay	Human
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Recombinant Human ICAM-1/CD54 Protein, CF

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Catalog# & Formulation	Size	Price
Carrier Free ADP4-200

Gene Symbol	ICAM1
Uniprot	CAA30051()