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Recombinant Human CXCL2/GRO beta Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Carrier Free
276-GB-010/CF
Carrier Free
276-GB-050/CF
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Recombinant Human CXCL2/GRO beta Protein Summary

Details of Functionality
Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474. The ED50 for this effect is 0.3‑0.9 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 3‑15 ng/mL.
Source
E. coli-derived human CXCL2/GRO beta/MIP-2/CINC-3 protein
Ala35-Asn107
Accession #
N-terminal Sequence
Ala35
Protein/Peptide Type
Recombinant Proteins
Gene
CXCL2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
276-GB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CXCL2/GRO beta Protein

  • chemokine (C-X-C motif) ligand 2
  • CINC-2a
  • CINC3
  • CINC-3
  • C-X-C Motif Chemokine 2
  • CXCL2
  • GRO beta
  • GRO2 oncogene
  • GRO2
  • GROB
  • Gro-beta
  • Growth-regulated protein beta
  • Macrophage inflammatory protein 2-alpha
  • melanoma growth stimulatory activity beta
  • MGSA beta
  • MGSA-b
  • MGSA-beta
  • MIP2
  • MIP-2
  • MIP2A
  • MIP-2a
  • MIP2-alpha
  • SCYB2

Background

Human GRO alpha , GRO beta (MIP-2 alpha ), and GRO gamma (MIP-2 beta ) are products of three distinct, non-allelic human genes. GRO beta and GRO gamma share 90% and 86% amino acid sequence homology, respectively, with GRO alpha . All three human GROs are members of the alpha (C-X-C) subfamily of chemokines and are thought to be the homologs of murine KC and MIP-2.

The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively.

Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

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Publications for CXCL2/GRO beta/MIP-2/CINC-3 (276-GB)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Binding Assay, Bioassay.


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Binding Assay
(1)
Bioassay
(5)
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Human
(4)
Mouse
(2)
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Showing Publications 1 - 6 of 6.
Publications using 276-GB Applications Species
VY Su, WC Chen, WK Yu, HH Wu, H Chen, KY Yang Nintedanib Regulates GRK2 and CXCR2 to Reduce Neutrophil Recruitment in Endotoxin-Induced Lung Injury International Journal of Molecular Sciences, 2021-09-13;22(18):. 2021-09-13 [PMID: 34576061] (Bioassay, Human) Bioassay Human
AC Cheng, KY Yang, NJ Chen, TL Hsu, R Jou, SL Hsieh, PH Tseng CLEC9A modulates macrophage-mediated neutrophil recruitment in response to heat-killed Mycobacterium tuberculosis H37Ra PLoS ONE, 2017-10-24;12(10):e0186780. 2017-10-24 [PMID: 29065139] (Bioassay, Human) Bioassay Human
Sukkurwala A, Martins I, Wang Y, Schlemmer F, Ruckenstuhl C, Durchschlag M, Michaud M, Senovilla L, Sistigu A, Ma Y, Vacchelli E, Sulpice E, Gidrol X, Zitvogel L, Madeo F, Galluzzi L, Kepp O, Kroemer G Immunogenic calreticulin exposure occurs through a phylogenetically conserved stress pathway involving the chemokine CXCL8. Cell Death Differ, 2013-06-21;21(1):59-68. 2013-06-21 [PMID: 23787997] (Bioassay, Mouse) Bioassay Mouse
Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis. EMBO J., 2012-07-31;31(17):3607-19. 2012-07-31 [PMID: 22850671] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, Vassileva G, Zeng M, Jackson C, Sullivan L, Sharif-Rodriguez W, Opdenakker G, Van Damme J, Hedrick JA, Lundell D, Lira SA, Hipkin RW Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8. J. Biol. Chem., 2006-12-29;282(16):11658-66. 2006-12-29 [PMID: 17197447] (Binding Assay, Mouse) Binding Assay Mouse

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Bioinformatics

Gene Symbol CXCL2
Uniprot