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Recombinant Human CXCL13/BLC/BCA-1 Protein, CF

 (20 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
801-CX-025
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Recombinant Human CXCL13/BLC/BCA-1 Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR5. The ED50 for this effect is 0.005‑0.02 µg/mL.
Source
E. coli-derived human CXCL13/BLC/BCA-1 protein
Val23-Arg94
Accession #
N-terminal Sequence
Val23
Protein/Peptide Type
Recombinant Proteins
Gene
CXCL13
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
8.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
9 kDa, reducing conditions
Publications
Read Publications using
801-CX/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CXCL13/BLC/BCA-1 Protein, CF

  • ANGIE
  • ANGIE2
  • B cell-attracting chemokine 1
  • B lymphocyte chemoattractant
  • BCA1
  • BCA-1
  • BCA1B-cell chemoattractant
  • BCA-1CXC chemokine BLC
  • B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
  • BLC
  • BLCSmall-inducible cytokine B13
  • BLR1L
  • B-lymphocyte chemoattractant
  • chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
  • chemokine (C-X-C motif) ligand 13
  • C-X-C motif chemokine 13
  • CXCL13
  • SCYB13
  • SCYB13B-cell-attracting chemokine 1
  • small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Background

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.

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Recombinant Human
CXCL13/BLC/BCA-1 Prot ...
801-CX/CF
Recombinant Human CXCL13/BLC/BCA-1 Protein, CF
Species: Hu
Applications: Bioactivity

Publications for CXCL13/BLC/BCA-1 (801-CX/CF)(20)

We have publications tested in 2 confirmed species: Human, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 3 applications: Antibody Absorption, Bioassay, ELISA (Standard).


Filter By Application
Antibody Absorption
(1)
Bioassay
(18)
ELISA (Standard)
(1)
All Applications
Filter By Species
Human
(17)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using 801-CX/CF Applications Species
CC Chao, WF Lee, SW Wang, PC Chen, A Yamamoto, TM Chang, SL Weng, JF Liu CXC chemokine ligand-13 promotes metastasis via CXCR5-dependent signaling pathway in non-small cell lung cancer Journal of Cellular and Molecular Medicine, 2021-08-24;0(0):. 2021-08-24 [PMID: 34427969] (Bioassay, Human) Bioassay Human
D Biasci, M Smoragiewi, CM Connell, Z Wang, Y Gao, JED Thaventhir, B Basu, L Magiera, TI Johnson, L Bax, A Gopinathan, C Isherwood, FA Gallagher, M Pawula, I Hudecova, D Gale, N Rosenfeld, P Barmpounak, EC Popa, R Brais, E Godfrey, F Mir, FM Richards, DT Fearon, T Janowitz, DI Jodrell CXCR4 inhibition in human pancreatic and colorectal cancers induces an integrated immune response Proc Natl Acad Sci U S A, 2020-10-30;0(0):. 2020-10-30 [PMID: 33127761] (Bioassay, Human) Bioassay Human
A Ardain, R Domingo-Go, S Das, SW Kazer, NC Howard, A Singh, M Ahmed, S Nhamoyebon, J Rangel-Mor, P Ogongo, L Lu, D Ramsuran, M de la Luz, T K Ulland, M Darby, E Park, F Karim, L Melocchi, R Madansein, KJ Dullabh, M Dunlap, N Marin-Agud, T Ebihara, T Ndung'u, D Kaushal, AS Pym, JK Kolls, A Steyn, J Zúñiga, W Horsnell, WM Yokoyama, AK Shalek, HN Kløverpris, M Colonna, A Leslie, SA Khader Group 3 innate lymphoid cells mediate early protective immunity against tuberculosis Nature, 2019-06-05;570(7762):528-532. 2019-06-05 [PMID: 31168092] (Bioassay, Human) Bioassay Human
LW Middleton, Z Shen, S Varma, AS Pollack, X Gong, S Zhu, C Zhu, JW Foley, S Vennam, RT Sweeney, K Tu, J Biscocho, O Eminaga, R Nolley, R Tibshirani, JD Brooks, RB West, JR Pollack Genomic analysis of benign prostatic hyperplasia implicates cellular re-landscaping in disease pathogenesis JCI Insight, 2019-05-16;5(0):. 2019-05-16 [PMID: 31094703] (Bioassay, Human) Bioassay Human
SD Reiff, R Mantel, LL Smith, JT Greene, EM Muhowski, CA Fabian, VM Goettl, M Tran, B Harrington, KA Rogers, FT Awan, K Maddocks, L Andritsos, AM Lehman, D Sampath, R Lapalombel, S Eathiraj, G Abbadessa, B Schwartz, A Johnson, JC Byrd, JA Woyach The BTK Inhibitor ARQ 531 Targets Ibrutinib Resistant CLL and Richter&#039;s Transformation Cancer Discov, 2018-08-09;0(0):. 2018-08-09 [PMID: 30093506] (Bioassay, Human) Bioassay Human
E Armas-Gonz, MJ Domínguez-, A Díaz-Martí, M Arce-Franc, J Castro-Her, G Danelon, V Hernández-, S Bustabad-R, A Cantabrana, M Uguccioni, F Díaz-Gonzá Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis Arthritis Res. Ther., 2018-06-07;20(1):114. 2018-06-07 [PMID: 29880013] (Bioassay, Human) Bioassay Human
JG Chen, X Liu, M Munshi, L Xu, N Tsakmaklis, MG Demos, A Kofides, ML Guerrera, GG Chan, CJ Patterson, KE Meid, J Gustine, T Dubeau, P Severns, JJ Castillo, ZR Hunter, J Wang, SJ Buhrlage, NS Gray, SP Treon, G Yang BTKCys481Serdrives ibrutinib resistance via ERK1/2, and protects BTKWild-TypeMYD88 mutated cells by a paracrine mechanism Blood, 2018-03-01;0(0):. 2018-03-01 [PMID: 29496671] (Bioassay, Human) Bioassay Human
Blackburn M, Zhong-Min M, Caccuri F, McKinnon K, Schifanella L, Guan Y, Gorini G, Venzon D, Fenizia C, Binello N, Gordon S, Miller C, Franchini G, Vaccari M Regulatory and Helper Follicular T Cells and Antibody Avidity to Simian Immunodeficiency Virus Glycoprotein 120. J Immunol, 2015-08-21;195(7):3227-36. 2015-08-21 [PMID: 26297759] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque)) Bioassay Primate - Macaca mulatta (Rhesus Macaque)
Boswell K, Paris R, Boritz E, Ambrozak D, Yamamoto T, Darko S, Wloka K, Wheatley A, Narpala S, McDermott A, Roederer M, Haubrich R, Connors M, Ake J, Douek D, Kim J, Petrovas C, Koup R Loss of circulating CD4 T cells with B cell helper function during chronic HIV infection. PLoS Pathog, 2014-01-30;10(1):e1003853. 2014-01-30 [PMID: 24497824] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Show All 20 Publications.

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Bioinformatics

Gene Symbol CXCL13
Uniprot