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CXCL13/BLC/BCA-1 Antibody [Unconjugated]

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Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more
CXCL13/BLC/BCA‑1 was detected in perfusion fixed frozen sections of mouse intestine (Peyer's patch) using Goat Anti-Mouse CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470) at 15 µg/mL ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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CXCL13/BLC/BCA-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CXCL13/BLC/BCA‑1
Ile22-Ala109
Accession # Q3U1E8
Specificity
Detects mouse CXCL13/BLC/BCA‑1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL13 and rhGCP-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCL13
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 20-50 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF470 in the following applications:

Publications
Read Publications using
AF470 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL13/BLC/BCA-1 Antibody [Unconjugated]

  • ANGIE
  • ANGIE2
  • B cell-attracting chemokine 1
  • B lymphocyte chemoattractant
  • BCA1
  • BCA-1
  • BCA1B-cell chemoattractant
  • BCA-1CXC chemokine BLC
  • B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
  • BLC
  • BLCSmall-inducible cytokine B13
  • BLR1L
  • B-lymphocyte chemoattractant
  • chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
  • chemokine (C-X-C motif) ligand 13
  • C-X-C motif chemokine 13
  • CXCL13
  • SCYB13
  • SCYB13B-cell-attracting chemokine 1
  • small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Background

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL13/BLC/BCA-1 Antibody (AF470)(60)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 12 applications: Bioassay, Flow Cytometry, ICC, IHC, IHC-F, IHC-Fr, IHC-P, Immunohistochemistry, In Vivo, Migration Assay, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(2)
ICC
(1)
IHC
(14)
IHC-F
(1)
IHC-Fr
(11)
IHC-P
(6)
Immunohistochemistry
(1)
In Vivo
(1)
Migration Assay
(1)
Neutralization
(2)
Western Blot
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(38)
All Species
Showing Publications 1 - 10 of 60. Show All 60 Publications.
Publications using AF470 Applications Species
Norman, KM;Lang, GA;Shadid, TM;Honold, ST;Reel, JM;Cox, MA;Ballard, JD;Lang, ML; Clostridioides difficile toxin B subverts germinal center and antibody recall responses by stimulating a drug-treatable CXCR4-dependent mechanism Cell reports 2024-05-17 [PMID: 38761377] (Neutralization, Mouse) Neutralization Mouse
Fitzpatrick, Z;Ghabdan Zanluqui, N;Rosenblum, JS;Tuong, ZK;Lee, CYC;Chandrashekhar, V;Negro-Demontel, ML;Stewart, AP;Posner, DA;Buckley, M;Allinson, KSJ;Mastorakos, P;Chittiboina, P;Maric, D;Donahue, D;Helmy, A;Tajsic, T;Ferdinand, JR;Portet, A;Peñalver, A;Gillman, E;Zhuang, Z;Clatworthy, MR;McGavern, DB; Venous-plexus-associated lymphoid hubs support meningeal humoral immunity Nature 2024-03-20 [PMID: 38509366] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Edith Marcial-Juárez, Marisol Pérez-Toledo, Saba Nayar, Elena Pipi, Areej Alshayea, Ruby Persaud et al. Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers iScience 4/21/2023 [PMID: 36950118]
Qizhi Ma, Yue Chen, Qing Qin, Fuchun Guo, Yong-sheng Wang, Dan Li CXCL13 expression in mouse 4T1 breast cancer microenvironment elicits antitumor immune response by regulating immune cell infiltration Precision Clinical Medicine 9/16/2021 [PMID: 35693216]
Aditya Rayasam, Julie A. Kijak, Lee Kissel, Yun Hwa Choi, Taehee Kim, Martin Hsu et al. CXCL13 expressed on inflamed cerebral blood vessels recruit IL-21 producing TFH cells to damage neurons following stroke Journal of Neuroinflammation 12/1/2022 [PMID: 35624463]
Wafaa Mahmoud, Alexander Perniss, Krupali Poharkar, Aichurek Soultanova, Uwe Pfeil, Andreas Hoek et al. CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung Cell and Tissue Research 10/1/2022 [PMID: 34762185]
Naoki Iwanaga, Kong Chen, Haoran Yang, Shiping Lu, Joseph P. Hoffmann, Alanna Wanek et al. Vaccine-driven lung TRM cells provide immunity against Klebsiella via fibroblast IL-17R signaling Science Immunology 9/24/2021 [PMID: 34516780]
Malgorzata Ciurkiewicz, Stefan Floess, Michael Beckstette, Maren Kummerfeld, Wolfgang Baumgärtner, Jochen Huehn et al. Transcriptome analysis following neurotropic virus infection reveals faulty innate immunity and delayed antigen presentation in mice susceptible to virus‐induced demyelination Brain Pathology 11/1/2021 [PMID: 34231271]
Samira Mansouri, Divya S. Katikaneni, Himanshu Gogoi, Lei Jin Monocyte-Derived Dendritic Cells (moDCs) Differentiate into Bcl6+ Mature moDCs to Promote Cyclic di-GMP Vaccine Adjuvant–Induced Memory TH Cells in the Lung The Journal of Immunology 5/1/2021 [PMID: 33879579]
MC Trolese, A Mariani, M Terao, M de Paola, P Fabbrizio, F Sironi, M Kurosaki, S Bonanno, S Marcuzzo, P Bernasconi, F Trojsi, E Aronica, C Bendotti, G Nardo CXCL13/CXCR5 signalling is pivotal to preserve motor neurons in amyotrophic lateral sclerosis EBioMedicine, 2020-11-09;62(0):103097. 2020-11-09 [PMID: 33161233] (IHC, Mouse) IHC Mouse
Show All 60 Publications.

Reviews for CXCL13/BLC/BCA-1 Antibody (AF470) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF470:
Filter by Applications
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IHC-Fr Mouse 01/08/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 20643338
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 21593383
SpeciesMouse

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
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Verified Customer
01/08/2015
Application: IHC-Fr
Species: Mouse

 
Verified Customer
01/08/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol CXCL13
Uniprot