Reactivity | MuSpecies Glossary |
Applications | WB, IHC, B/N |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | E. coli-derived recombinant mouse CXCL13/BLC/BCA‑1 Ile22-Ala109 Accession # Q3U1E8 |
Specificity | Detects mouse CXCL13/BLC/BCA‑1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL13 and rhGCP-2 is observed. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | CXCL13 |
Purity Statement | Antigen Affinity-purified |
Endotoxin Note | <0.10 EU per 1 μg of the antibody by the LAL method. |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.
Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
IHC-Fr | Mouse | 01/08/2015 |
Summary
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reviewed by:
Verified Customer |
IF | Mouse | 01/08/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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Verified Customer 01/08/2015 |
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Application: | IHC-Fr | |
Species: | Mouse |
Verified Customer 01/08/2015 |
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Application: | IF | |
Species: | Mouse |