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PTRF Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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PTRF Antibody Summary

Description
This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
PTRF Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of human PTRF. (Uniprot: Q6NZI2)
Localization
Plasma membrane and caveolae of adipocytes
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAVIN1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:20000-1:60000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Western Blot 1 ug/ml
Application Notes
This product is useful for ELISA, Immunostaining, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Protein Molecular weight: 60 kDa. Expect a band approximately ~40.5 kDa corresponding to the appropriate cell lysate or extract.
Publications
Read Publications using NBP1-44089.

Reactivity Notes

Cross-reactivity with PTRF (polymerase I and transcript release factor) from other sources has not been determined

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for PTRF Antibody

  • CAVIN
  • cavin-1
  • CGL4
  • FLJ90031
  • polymerase I and transcript release factor
  • RNA polymerase I and transcript release factor
  • TTF-I interacting peptide 12

Background

PTRF (polymerase I and transcript release factor) was first identified as a TTF-I and RNA polymerase I (RNA Pol I) interacting protein that functions in the termination of RNA polymerase I transcription. PTRF has also been described as a protein that localizes to the plasma membrane and caveolae of adipocytes and whose localization is under the control of insulin. In this context, PTRF has been observed to associate with a lipase and have an extranuclear role in the regulation of lipolysis. PTRF is also known as FKSG13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PTRF Antibody (NBP1-44089)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PTRF Antibody (NBP1-44089) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on PTRF

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Bioinformatics

Gene Symbol CAVIN1
Entrez
Uniprot