PTRF Antibody Summary
Description |
This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
Immunogen |
PTRF Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of human PTRF. (Uniprot: Q6NZI2) |
Localization |
Plasma membrane and caveolae of adipocytes |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
CAVIN1 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA 1:20000-1:60000
- Immunocytochemistry/ Immunofluorescence 1:100-1:500
- Immunohistochemistry 1:100-1:500
- Western Blot 1 ug/ml
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Application Notes |
This product is useful for ELISA, Immunostaining, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Protein Molecular weight: 60 kDa. Expect a band approximately ~40.5 kDa corresponding to the appropriate cell lysate or extract. |
Publications |
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Reactivity Notes
Cross-reactivity with PTRF (polymerase I and transcript release factor) from other sources has not been determined
Packaging, Storage & Formulations
Storage |
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol |
Preservative |
No Preservative |
Purity |
Immunogen affinity purified |
Alternate Names for PTRF Antibody
Background
PTRF (polymerase I and transcript release factor) was first identified as a TTF-I and RNA polymerase I (RNA Pol I) interacting protein that functions in the termination of RNA polymerase I transcription. PTRF has also been described as a protein that localizes to the plasma membrane and caveolae of adipocytes and whose localization is under the control of insulin. In this context, PTRF has been observed to associate with a lipase and have an extranuclear role in the regulation of lipolysis. PTRF is also known as FKSG13.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for PTRF Antibody (NBP1-44089)(2)
Showing Publications 1 -
2 of 2.
Publications using NBP1-44089 |
Applications |
Species |
Bastiani M, Liu L, Hill MM et al. MURC/Cavin-4 and cavin family members form tissue-specific caveolar complexes. J Cell Biol 2009-06-01 [PMID: 19546242] |
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Hill MM, Bastiani M, Luetterforst R, Kirkham M, Kirkham A, Nixon SJ, Walser P, Abankwa D, Oorschot VM, Martin S, Hancock JF, Parton RG, PTRF-Cavin. a conserved cytoplasmic protein required for caveola formation and function. Cell 2008-01-11 [PMID: 18191225] |
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Secondary Antibodies
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