![Western Blot: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of Dysferlin on human skeletal muscle lysates using anti-Dysferlin antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Western-Blot-NBP2-66814-img0005.jpg "Western Blot: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of Dysferlin on human skeletal muscle lysates using anti-Dysferlin antibody at 1/1,000 dilution.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | JF05-54 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Dysferlin aa 113-162 / 2080. (SwissProt: O75923 Human; SwissProt: Q9ESD7 Mouse) |
| Localization | Cell membrane, Cytoplasmic vesicle membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | DYSF |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 237 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Dysferlin Antibody (NBP2-66814)Find related products by research area.
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| Gene Symbol | DYSF |
![Immunocytochemistry/Immunofluorescence: Dysferlin Antibody (JF05-54) [NBP2-66814] - Staining Dysferlin in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Immunocytochemistry-Immunofluorescence-NBP2-66814-img0006.jpg "Immunocytochemistry/Immunofluorescence: Dysferlin Antibody (JF05-54) [NBP2-66814] - Staining Dysferlin in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Dysferlin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Immunohistochemistry-Paraffin-NBP2-66814-img0007.jpg "Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Dysferlin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of paraffin-embedded human spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Immunohistochemistry-Paraffin-NBP2-66814-img0001.jpg "Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of paraffin-embedded human spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of paraffin-embedded mouse heart tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Immunohistochemistry-Paraffin-NBP2-66814-img0002.jpg "Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of paraffin-embedded mouse heart tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Immunohistochemistry-Paraffin-NBP2-66814-img0003.jpg "Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of paraffin-embedded mouse spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Immunohistochemistry-Paraffin-NBP2-66814-img0004.jpg "Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of paraffin-embedded mouse spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")