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MURC Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: MURC Antibody [NBP1-86609] - Staining in human skeletal muscle and pancreas tissues using anti-MURC antibody. Corresponding MURC RNA-seq data are presented ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: MURC Antibody [NBP1-86609] - Staining of human cerebral cortex, colon, pancreas and skeletal muscle using Anti-MURC antibody NBP1-86609 (A) shows similar ...read more
Western Blot: MURC Antibody [NBP1-86609] - Analysis in control (vector only transfected HEK293T lysate) and MURC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MURC Antibody [NBP1-86609] - Staining of human cell line RH-30 shows localization to nucleoplasm, plasma membrane & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MURC Antibody [NBP1-86609] - Staining of human colon.
Immunohistochemistry-Paraffin: MURC Antibody [NBP1-86609] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: MURC Antibody [NBP1-86609] - Staining of human skeletal muscle shows high expression.
Immunohistochemistry-Paraffin: MURC Antibody [NBP1-86609] - Staining of human cerebral cortex.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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MURC Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KGKDRTVAEGEECAREMGVDIIARSESLGPISELYSDELSEPEHEAARPVYPPHEGREIPTPEPLKVTFKSQVKVEDDESLLL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MURC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
MURC Protein (NBP1-86609PEP)
Publications
Read Publication using
NBP1-86609 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26086601).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MURC Antibody

  • muscle-related coiled-coil protein
  • Muscle-restricted coiled-coil protein

Background

Induces RHOA activation and activates NPPA transcription and myofibrillar organization through the Rho/ROCKsignaling pathway

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MURC Antibody (NBP1-86609)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MURC