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PPP2R4 Antibody (529245)

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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Raji human Burkitt's lymphoma cell line, CH-1 mouse B cell lymphoma cell line, BaF3 mouse pro-B cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat PPP2R4 Monoclonal Antibody (Catalog # MAB5929) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PPP2R4 at approximately 39 to 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Raji human Burkitt's lymphoma cell line, CH-1 mouse B cell lymphoma cell line, BaF3 mouse pro-B cell line, and NRK rat normal kidney cell ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
529245
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5929-SP
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PPP2R4 Antibody (529245) Summary

Immunogen
E. coli-derived recombinant human PPP2R4
Glu14-Asn323
Accession # Q15257
Specificity
Detects endogenous human, mouse and rat PPP2R4 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PPP2R4
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PPP2R4 Antibody (529245)

  • EC 5.2.1.8
  • MGC2184
  • Phosphotyrosyl phosphatase activator
  • PP2A phosphatase activator
  • PP2A subunit B' isoform PR53
  • PP2A
  • PP2A, subunit B', PR53 isoform
  • PPP2R4
  • protein phosphatase 2A activator, regulatory subunit 4
  • PTPA
  • regulatory subunit B' (PR 53)
  • serine/threonine-protein phosphatase 2A activator
  • Serine/threonine-protein phosphatase 2A regulatory subunit 4
  • Serine/threonine-protein phosphatase 2A regulatory subunit B'

Background

PPP2R4, also known as serine/threonine protein phosphatase 2A regulatory subunit B’, PR53 isoform, and PTPA, is a widely expressed member of the PTPA-type peptidyl prolyl isomerase family. Human PPP2R4 is 358 amino acids (aa) in length. Three splicing variants produce four isoforms for PPP2R4, and isoform 2 is the standard protein. Isoform 1, isoform 3 and isoform 4 have deletions corresponding to aa 73-107, 45-108, and 73-149 in isoform 2, respectively. Human PPP2R4 is 87% aa identical to mouse and rabbit PPP2R4. PPP2R4 reversibly stimulates the variable phosphotyrosyl phosphatase activity of the PP2A core heterodimer in the presence of ATP and Mg2+. It has been shown that p53 downregulates the expression of PPP2R4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PPP2R4
Uniprot