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POU3F2/OCT7 Antibody

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Western Blot: POU3F2/OCT7 Antibody [NBP2-21585] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Brn2 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: POU3F2/OCT7 Antibody [NBP2-21585] - SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brn2 protein stained by Brn2 antibody diluted at 1:1000. Red: beta ...read more
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585] - Rat brain. Brn2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Chromatin Immunoprecipitation: POU3F2/OCT7 Antibody [NBP2-21585] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of OCT7 antibody The precipitated DNA was detected by PCR with primer set ...read more
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585] - TOV-21G xenograft, using Brn2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Frozen: POU3F2/OCT7 Antibody [NBP2-21585] - Frozen sectioned E13.5 Rat brain. Green: Brn2 protein stained by Brn2 antibody ( diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, ...read more
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585] - Mouse brain. Brn2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: POU3F2/OCT7 Antibody [NBP2-21585] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Bv, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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POU3F2/OCT7 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human POU3F2/OCT7. The exact sequence is proprietary.
Predicted Species
Xenopus (97%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POU3F2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) Assay-dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-21585 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for POU3F2/OCT7 Antibody

  • Brain-2
  • Brain-specific homeobox/POU domain protein 2
  • BRN2
  • brn-2
  • BRN2brain-2
  • Nervous system-specific octamer-binding transcription factor N-Oct-3
  • Oct7
  • oct-7
  • OCT7POU domain class 3, transcription factor 2
  • Octamer-binding protein 7
  • Octamer-binding transcription factor 7
  • OTF7
  • OTF-7
  • POU class 3 homeobox 2
  • POU domain, class 3, transcription factor 2
  • POU3F2
  • POUF3

Background

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for POU3F2/OCT7 Antibody (NBP2-21585)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for POU3F2/OCT7 Antibody (NBP2-21585) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POU3F2
Uniprot