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Pax3/Pax7 Antibody (274212) [Unconjugated]

Images

 
Pax3 was detected in immersion fixed B16-F1 mouse melanoma cell line using Mouse Anti-Human/Mouse Pax3 Monoclonal Antibody (Catalog # MAB2457) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more
Characterization of iMPCs during monolayer differentiation. a–e Representative immunostaining of Pax3 (a), Myf5 (b), MyoD (c), and MyoG (d), and corresponding quantification (e) during iMPC expansion. Scale ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clone
274212
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Pax3/Pax7 Antibody (274212) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Pax3 (isoform Pax3a)
Met1-Ser215
Accession # NP_000429
Specificity
Detects human and mouse Pax3 and Pax7 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Pax1 is observed, approximately 10% with rhPax6, and less than 2% with rhPax2, rhPax4, rhPax5, and rhPax9.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PAX3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB2457 in the following applications:

Publications
Read Publications using
MAB2457 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax3/Pax7 Antibody (274212) [Unconjugated]

  • Pax3/Pax7

Background

Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. Human and mouse Pax3 share 98% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pax3/Pax7 Antibody (MAB2457)(19)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, Functional Assay, ICC, IHC, Western Blot.


Filter By Application
Flow Cytometry
(1)
Functional Assay
(1)
ICC
(5)
IHC
(3)
Western Blot
(5)
All Applications
Filter By Species
Human
(9)
Mouse
(7)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using MAB2457 Applications Species
Chauhan, S;Lian, E;Habib, I;Liu, Q;Anders, NM;Bugg, MM;Federman, NC;Reid, JM;Stewart, CF;Cates, T;Michalek, JE;Keller, C; Entinostat as a combinatorial therapeutic for rhabdomyosarcoma Scientific reports 2024-08-15 [PMID: 39147820] (Western Blot, Human) Western Blot Human
Karim Azzag, Darko Bosnakovski, Sudheer Tungtur, Peter Salama, Michael Kyba, Rita C. R. Perlingeiro Transplantation of PSC-derived myogenic progenitors counteracts disease phenotypes in FSHD mice npj Regenerative Medicine 2022-09-02 [PMID: 36056021]
M Ramirez, Y Badayeva, J Yeung, J Wu, A Abdalla-Wy, E Yang, FANTOM 5 C, B Trost, SW Scherer, D Goldowitz Temporal analysis of enhancers during mouse cerebellar development reveals dynamic and novel regulatory functions Elife, 2022-08-09;11(0):. 2022-08-09 [PMID: 35942939] (IHC, Mouse) IHC Mouse
L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State Cell Stem Cell, 2020-08-11;0(0):. 2020-08-11 [PMID: 32783886] (Flow Cytometry, ICC, IHC, Human) Flow Cytometry, ICC, IHC Human
Kenneth A. Crawford, Noah E. Berlow, Jennifer Tsay, Michael Lazich, Maria Mancini, Christopher Noakes et al. Case report for an adolescent with germline RET mutation and alveolar rhabdomyosarcoma Cold Spring Harb Mol Case Stud 2020-06-01 [PMID: 32532875]
Oliver A. Stone, Didier Y.R. Stainier Paraxial Mesoderm Is the Major Source of Lymphatic Endothelium Developmental Cell 2019-07-22 [PMID: 31130354]
Narendra Bharathy, Noah E. Berlow, Eric Wang, Jinu Abraham, Teagan P. Settelmeyer, Jody E. Hooper et al. The HDAC3–SMARCA4–miR-27a axis promotes expression of the PAX3:FOXO1 fusion oncogene in rhabdomyosarcoma Science Signaling 2018-11-22 [PMID: 30459282]
Matthew J. McBride, John L. Pulice, Hannah C. Beird, Davis R. Ingram, Andrew R. D’Avino, Jack F. Shern et al. The SS18-SSX Fusion Oncoprotein Hijacks BAF Complex Targeting and Function to Drive Synovial Sarcoma Cancer Cell 2018-06-01 [PMID: 29861296]
V Sahakyan, E Pozzo, R Duelen, J Deprest, M Sampaolesi Methotrexate and Valproic Acid Affect Early Neurogenesis of Human Amniotic Fluid Stem Cells from Myelomeningocele Stem Cells Int, 2017-09-13;2017(0):6101609. 2017-09-13 [PMID: 29056972] (ICC, Human) ICC Human
Mattia Quattrocelli, Melissa Swinnen, Giorgia Giacomazzi, Jordi Camps, Ines Barthélemy, Gabriele Ceccarelli et al. Mesodermal iPSC–derived progenitor cells functionally regenerate cardiac and skeletal muscle Journal of Clinical Investigation 2015-11-16 [PMID: 26571398]
Show All 19 Publications.

Review for Pax3/Pax7 Antibody (MAB2457) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using MAB2457:
Filter by Applications
WB
(1)
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Filter by Species
Other
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Other 02/19/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 21078671
SpeciesOther

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Verified Customer
02/19/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol PAX3
Uniprot