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FABP7/B-FABP Antibody [Unconjugated]

 (14 Publications)
 (2 Reviews)
 
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FABP7 was detected in immersion fixed paraffin-embedded sections of human melanoma using 5 µg/mL Goat Anti-Human FABP7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
FABP7 was detected in immersion fixed paraffin-embedded sections of human melanoma using 5 µg/mL Goat Anti-Human FABP7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of human cerebellum tissue and human hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP7/B‑FABP at approximately 18 kDa (as indicated) using 50 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Carrier Free
AF3166-SP
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FABP7/B-FABP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human FABP7/B-FABP
Met1-Ala132
Accession # O15540
Specificity
Detects human FABP7/B-FABP in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FABP1, rhFABP2, rhFABP3, rhFABP4, rhFABP5, rhFABP6, rhFABP8, rhFABP9 and recombinant mouse FABP9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FABP7
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF3166 in the following applications:
Publications
Read Publications using
AF3166 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP7/B-FABP Antibody [Unconjugated]

  • BFABP
  • B-FABP
  • B-FABPDKFZp547J2313
  • BLBP
  • BLBPMRG
  • Brain lipid-binding protein
  • Brain-type fatty acid-binding protein
  • FABP7
  • FABPBbrain lipid binding protein
  • fatty acid binding protein 7, brain
  • Fatty acid-binding protein 7
  • fatty acid-binding protein, brain
  • Mammary-derived growth inhibitor related
  • mammary-derived growth inhibitor-related

Background

FABP7, also known as (Brain) B-FABP, Brain lipid-binding protein (BLBP), and mammary-derived growth inhibitor-related (MRG), belongs to the family of intracellular lipid-binding proteins. It is involved in fatty acid uptake and intracellular transport and is important in brain development. Human and mouse FABP7 share 86% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FABP7/B-FABP Antibody (AF3166)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Macaca fuscata (Japanese Macaque).

We have publications tested in 6 applications: ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(2)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(5)
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Human
(3)
Mouse
(5)
Primate - Macaca fuscata (Japanese Macaque)
(1)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF3166 Applications Species
Sharma S, Littman R, Tompkins JD et al. Tiered sympathetic control of cardiac function revealed by viral tracing and single cell transcriptome profiling eLife 5/10/2023 [PMID: 37162194]
S Sharma, R Littman, J Tompkins, D Arneson, J Contreras, AH Dajani, K Ang, A Tsanhani, X Sun, PY Jay, H Herzog, X Yang, OA Ajijola Tiered Sympathetic Control of Cardiac Function Revealed by Viral Tracing and Single Cell Transcriptome Profiling bioRxiv : the preprint server for biology, 2023-01-19;0(0):. 2023-01-19 [PMID: 36711942] (IHC, Mouse) IHC Mouse
MA Hunt, H Lund, L Delay, GG Dos Santos, A Pham, Z Kurtovic, A Telang, A Lee, A Parvathane, E Kussick, M Corr, TL Yaksh DRGquant: A new modular AI-based pipeline for 3D analysis of the DRG Journal of neuroscience methods, 2022-02-16;371(0):109497. 2022-02-16 [PMID: 35181343] (IHC, Mouse) IHC Mouse
A Cheng, W Jia, I Kawahata, K Fukunaga A novel fatty acid-binding protein 5 and 7 inhibitor ameliorates oligodendrocyte injury in multiple sclerosis mouse models EBioMedicine, 2021-10-05;72(0):103582. 2021-10-05 [PMID: 34624687] (Western Blot, Mouse) Western Blot Mouse
Kjeld Morten Mohr, Lone Tjener Pallesen, Mette Richner, Christian Bjerggaard Vaegter Discrepancy in the Usage of GFAP as a Marker of Satellite Glial Cell Reactivity Biomedicines 8/16/2021 [PMID: 34440226]
Q Guo, I Kawahata, T Degawa, Y Ikeda-Mats, M Sun, F Han, K Fukunaga Fatty Acid-Binding Proteins Aggravate Cerebral Ischemia-Reperfusion Injury in Mice Biomedicines, 2021-05-10;9(5):. 2021-05-10 [PMID: 34068550] (Western Blot, Mouse) Western Blot Mouse
A Cheng, I Kawahata, K Fukunaga Fatty Acid Binding Protein 5 Mediates Cell Death by Psychosine Exposure through Mitochondrial Macropores Formation in Oligodendrocytes Biomedicines, 2020-12-20;8(12):. 2020-12-20 [PMID: 33419250] (ICC, Immunoprecipitation, Western Blot, Human) ICC, Immunoprecipitation, Western Blot Human
K Zhang, F Yu, J Zhu, S Han, J Chen, X Wu, Y Chen, T Shen, J Liao, W Guo, X Yang, R Wang, Y Qian, J Yang, L Cheng, Y Zhao, CC Hui, J Li, G Peng, S He, N Jing, K Tang Imbalance of Excitatory/Inhibitory Neuron Differentiation in Neurodevelopmental Disorders with an NR2F1 Point Mutation Cell Rep, 2020-04-21;31(3):107521. 2020-04-21 [PMID: 32320667] (ICC, Human) ICC Human
Lan B Hoang‐Minh, Florian A Siebzehnrubl, Changlin Yang, Silveli Suzuki‐Hatano, Kyle Dajac, Tyler Loche et al. Infiltrative and drug‐resistant slow‐cycling cells support metabolic heterogeneity in glioblastoma The EMBO Journal 12/3/2018 [PMID: 30322894]
T Owa, S Taya, S Miyashita, M Yamashita, T Adachi, K Yamada, M Yokoyama, S Aida, T Nishioka, YU Inoue, R Goitsuka, T Nakamura, T Inoue, K Kaibuchi, M Hoshino Meis1 coordinates cerebellar granule cell development by regulating Pax6 transcription, BMP signaling and Atoh1 degradation J. Neurosci., 2018-01-09;0(0):. 2018-01-09 [PMID: 29317485] (IHC-P, Mouse) IHC-P Mouse
Show All 14 Publications.

Reviews for FABP7/B-FABP Antibody (AF3166) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using AF3166:
Filter by Applications
WB
(1)
ELISA
(1)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 WB Rat 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21771320
SpeciesRat
  4
reviewed by:
Verified Customer
 ELISA Human 01/07/2015
View

Summary

ApplicationELISA
Sample TestedSee PMID 22646866
SpeciesHuman

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Recent Reviews

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5
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4
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1
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 Verified Customer
01/07/2015
Application: WB
Species: Rat
read this review
 
 Verified Customer
01/07/2015
Application: ELISA
Species: Human
read this review

Bioinformatics

Gene Symbol FABP7
Uniprot