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![Immunocytochemistry/Immunofluorescence: POU3F2/OCT7 Antibody [NBP2-21585] - SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brn2 protein stained by Brn2 antibody diluted at 1:1000. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Scale bar = 10 um.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-21585-img0010.jpg "Immunocytochemistry/Immunofluorescence: POU3F2/OCT7 Antibody [NBP2-21585] - SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brn2 protein stained by Brn2 antibody diluted at 1:1000. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585] - Rat brain. Brn2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-Immunohistochemistry-Paraffin-NBP2-21585-img0007.jpg)
![Chromatin Immunoprecipitation: POU3F2/OCT7 Antibody [NBP2-21585] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of OCT7 antibody The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.](http://images.novusbio.com/fullsize/OCT7-Antibody-Chromatin-Immunoprecipitation-NBP2-21585-img0004.jpg)
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585] - TOV-21G xenograft, using Brn2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/OCT7-Antibody-Immunohistochemistry-Paraffin-NBP2-21585-img0002.jpg)
![Immunohistochemistry-Frozen: POU3F2/OCT7 Antibody [NBP2-21585] - Frozen sectioned E13.5 Rat brain. Green: Brn2 protein stained by Brn2 antibody ( diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [1710] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-Immunohistochemistry-Frozen-NBP2-21585-img0005.jpg)
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585] - Mouse brain. Brn2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-Immunohistochemistry-Paraffin-NBP2-21585-img0006.jpg)
![Immunoprecipitation: POU3F2/OCT7 Antibody [NBP2-21585] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of OCT7 protein by 2 ug of Brn2 antibody 10% SDS-PAGE The immunoprecipitated OCT7 protein was detected by OCT7. antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/OCT7-Antibody-Immunoprecipitation-NBP2-21585-img0003.jpg)
![Western Blot: POU3F2/OCT7 Antibody [NBP2-21585] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with POU3F2/OCT7 antibody (NBP2-21585) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-21585_rabbit-polyclonal-pou3f2-oct7-antibody-91020242041295.jpg)
![Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-POU3F2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Western-Blot-NBP2-61436-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-POU3F2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Staining of U-251 cells using the Anti-POU3F2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Immunocytochemistry-Immunofluorescence-NBP2-61436-img0001.jpg)
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Staining of mouse forebrain shows nuclear immunoreactivity in a subset of cells in the lateral ventricle wall.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Immunohistochemistry-Paraffin-NBP2-61436-img0004.jpg)
![Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Western-Blot-NBP2-61436-img0010.jpg)
![Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Western-Blot-NBP2-61436-img0009.jpg)
![Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Western-Blot-NBP2-61436-img0008.jpg)
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Staining of mouse cerebral cortex shows moderate nuclear positivity in neurons in layers 2-4.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Immunohistochemistry-Paraffin-NBP2-61436-img0002.jpg)
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Staining of mouse cerebral cortex shows absence of positivity in layer 6 neurons as expected.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Immunohistochemistry-Paraffin-NBP2-61436-img0003.jpg)
![Western Blot: POU3F2/OCT7 Antibody [NBP2-24658] - Analysis of POU3F2 in human Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse NIH 3T3 cell lysate in absence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/OCT7-Antibody-Western-Blot-NBP2-24658-img0002.jpg "Western Blot: POU3F2/OCT7 Antibody [NBP2-24658] - Analysis of POU3F2 in human Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse NIH 3T3 cell lysate in absence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-24658] - Human small intestine stained with POU3F2 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/OCT7-Antibody-Immunohistochemistry-Paraffin-NBP2-24658-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of U-251 cells using the Anti-POU3F2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6232-Immunocytochemistry-Immunofluorescence-NBP2-61437-img0001.jpg "Immunocytochemistry/Immunofluorescence: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of U-251 cells using the Anti-POU3F2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of rat cerebral cortex shows absence of positivity in layer 6 neurons as expected.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6232-Immunohistochemistry-Paraffin-NBP2-61437-img0008.jpg)
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of mouse forebrain shows nuclear immunoreactivity in layers 2-5 of cerebral cortex.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6232-Immunohistochemistry-Paraffin-NBP2-61437-img0002.jpg)
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of mouse forebrain shows nuclear immunoreactivity in a subset of cells in the lateral ventricle wall.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6232-Immunohistochemistry-Paraffin-NBP2-61437-img0005.jpg)
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of rat forebrain shows nuclear immunoreactivity in layers 2-5 of cerebral cortex.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6232-Immunohistochemistry-Paraffin-NBP2-61437-img0006.jpg)
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of rat cerebral cortex shows moderate nuclear positivity in neurons in layers 3-4.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6232-Immunohistochemistry-Paraffin-NBP2-61437-img0007.jpg)
![SDS-Page: Recombinant Human POU3F2/OCT7 Protein [H00005454-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005454-P01-1.jpg "SDS-Page: Recombinant Human POU3F2/OCT7 Protein [H00005454-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: POU3F2/OCT7 Antibody [NBP2-55453] - Staining of human cell line AF22 shows localization to nucleus.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55453-img0002.jpg "Immunocytochemistry/Immunofluorescence: POU3F2/OCT7 Antibody [NBP2-55453] - Staining of human cell line AF22 shows localization to nucleus.")
![Western Blot: OCT7 Overexpression Lysate (Adult Normal) [NBP2-10458] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OCT7.](http://images.novusbio.com/fullsize/OCT7-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10458-img0001.jpg "Western Blot: OCT7 Overexpression Lysate (Adult Normal) [NBP2-10458] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OCT7.")