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POU3F2/OCT7 Antibody (CL6232)

Images

 
Immunocytochemistry/ Immunofluorescence: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of U-251 cells using the Anti-POU3F2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule ...read more
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of rat cerebral cortex shows absence of positivity in layer 6 neurons as expected.
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of mouse forebrain shows nuclear immunoreactivity in layers 2-5 of cerebral cortex.
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of mouse forebrain shows nuclear immunoreactivity in a subset of cells in the lateral ventricle wall.
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of rat forebrain shows nuclear immunoreactivity in layers 2-5 of cerebral cortex.
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of rat cerebral cortex shows moderate nuclear positivity in neurons in layers 3-4.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clone
CL6232
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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POU3F2/OCT7 Antibody (CL6232) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ASNHYSLLTSSASIVHAEPPGGMQQGAGGYREAQSLVQGDYGALQSNGHP
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
POU3F2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for POU3F2/OCT7 Antibody (CL6232)

  • Brain-2
  • Brain-specific homeobox/POU domain protein 2
  • BRN2
  • brn-2
  • BRN2brain-2
  • Nervous system-specific octamer-binding transcription factor N-Oct-3
  • Oct7
  • oct-7
  • OCT7POU domain class 3, transcription factor 2
  • Octamer-binding protein 7
  • Octamer-binding transcription factor 7
  • OTF7
  • OTF-7
  • POU class 3 homeobox 2
  • POU domain, class 3, transcription factor 2
  • POU3F2
  • POUF3

Background

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on POU3F2/OCT7.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

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Bioinformatics

Gene Symbol POU3F2