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OCT2 Antibody (OCT2/2137)

Images

 
Western Blot: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Analysis (A) Recombinant Protein (B) Daudi cell lysate Oct-2 Mouse Monoclonal Antibody (OCT2/2137).
Immunohistochemistry-Paraffin: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Formalin-fixed, paraffin-embedded human Tonsil stained with Oct-2 Mouse Monoclonal Antibody (OCT2/2137).
Western Blot: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Analysis Ramos cell lysate using Oct-2 Mouse Monoclonal Antibody (OCT2/2137).
Immunohistochemistry-Paraffin: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Formalin-fixed, paraffin-embedded human Lymph Node stained with Oct-2 Mouse Monoclonal Antibody (OCT2/2137).
Protein Array: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Analysis of Protein Array containing more than 19,000 full-length human proteins using OCT2 Antibody (Oct2/2137). Z- and S- Score: The Z-score represents the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, MA
Clone
OCT2/2137
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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OCT2 Antibody (OCT2/2137) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-75735)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant fragment of human OCT2 protein (around aa 112-297) (exact sequence is proprietary) (Uniprot: P09086)
Localization
Nuclear
Marker
B-Cell Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POU2F2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for OCT2 Antibody (OCT2/2137)

  • homeobox protein
  • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2
  • OCT2Oct-2
  • Octamer-binding protein 2
  • Octamer-binding transcription factor 2
  • OTF-2
  • OTF2POU domain class 2, transcription factor 2
  • POU class 2 homeobox 2
  • POU domain, class 2, transcription factor 2

Background

Octamer transcription factor-2 (Oct-2) is a transcription factor that specifically binds to the octamer motif (5'- ATTTCAT-3'). Mostly expressed in B cells, Oct-2 posses a "leucine zipper" domain and belongs to the POU family of transcription factors (1-3). Additionally, Oct-2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POU2F2
Uniprot