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PKLR Antibody

Images

 
Western Blot: PKLR Antibody [NBP2-58892] - Analysis in human cell line K562.
Immunocytochemistry/ Immunofluorescence: PKLR Antibody [NBP2-58892] - Staining of human cell line Hep G2 shows localization to endoplasmic reticulum & the Golgi apparatus. Antibody staining is shown in green.
Western Blot: PKLR Antibody [NBP2-58892] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PKLR Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PKLR
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PKLR Recombinant Protein Antigen (NBP2-58892PEP)

Reactivity Notes

Mouse 86%, Rat 87%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PKLR Antibody

  • EC 2.7.1.40
  • PK1
  • PK1PKRL
  • PKL
  • PKLR
  • PKR
  • Pyruvate Kinase 1
  • pyruvate kinase isozyme R/L
  • pyruvate kinase isozymes R/L
  • pyruvate kinase type L
  • pyruvate kinase, liver and blood cell
  • pyruvate kinase, liver and RBC
  • Red cell/liver pyruvate kinase
  • RPK
  • R-type/L-type pyruvate kinase

Background

The protein encoded by the Pyruvate Kinase gene is a pyruvate kinase that catalyzes the transphosphorylation of phosphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKLR Antibody (NBP2-58892) (0)

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Bioinformatics

Gene Symbol PKLR