PKM2 Antibody - BSA Free

PKM2 (pyruvate kinase isozymes M1/M2), an isoform of pyruvate kinase, is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP and is expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. Other three pyruvate kinases includes PKL (liver and kidney), PKR (erythrocytes) and PKM1, (adult muscle, brain, bladder, and fibroblasts) which exist as stable tetramers, whereas, PKM2 subunits form tetramers as well as dimers, the dimeric form with higher Km value for PEP substrate stays inactive at physiological concentrations of PEP. The ratio between highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. Predominately localized in the cytoplasm, PKM2 translocate to nucleus upon apoptotic stimuli and this translocation mediated cell death is caspase independent, isoform-specific and independent of its enzymatic activity.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Bovine.

We have publications tested in 6 applications: ICC/IF, IHC-Fr, IHC-P, IP, WB, Western Blot.

Showing Publications 1 - 10 of 17. Show All 17 Publications. Collapse Publications.

Publications using NBP1-48308	Applications	Species
Li H, Guglielmetti C, Sei YJ et al. Neurons require glucose uptake and glycolysis in vivo Cell reports 2023-04-06 [PMID: 37027294] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Cheng Z, Qin H, Cao W et al. Intravoxel incoherent motion imaging used to assess tumor microvascular changes after transarterial chemoembolization in a rabbit VX2 liver tumor model Frontiers in Oncology 2023-02-28 [PMID: 36925931] (Western Blot)	Western Blot
Li Y, Chen X, Huang H et al. A feedback loop between NONHSAT024276 and PTBP1 inhibits tumor progression and glycolysis in HCC by increasing the PKM1/PKM2 ratio Cancer Science 2023-04-01 [PMID: 36529521] (Western Blot)	Western Blot
Zhang H, Wang D et al. Metabolic and Proliferative State of Vascular Adventitial Fibroblasts in Pulmonary Hypertension Is Regulated Through a MicroRNA-124/PTBP1 (Polypyrimidine Tract Binding Protein 1)/Pyruvate Kinase Muscle Axis. Circulation 2017-12-19 [PMID: 28972001] (WB, Bovine)	WB	Bovine
Sung WW, Chen PR, Liao MH, Lee JW. Enhanced aerobic glycolysis of nasopharyngeal carcinoma cells by Epstein-Barr virus latent membrane protein 1 Exp. Cell Res. 2017-08-04 [PMID: 28827059] (WB, Human)	WB	Human
Minami K, Taniguchi K, Sugito N et al. MiR-145 negatively regulates Warburg effect by silencing KLF4 and PTBP1 in bladder cancer cells. Oncotarget. 2017-05-16 [PMID: 28380435] (WB, Human)	WB	Human
Takai T, Yoshikawa Y, Inamoto T et al. A Novel Combination RNAi toward Warburg Effect by Replacement with miR-145 and Silencing of PTBP1 Induces Apoptotic Cell Death in Bladder Cancer Cells Int J Mol Sci 2017-01-17 [PMID: 28106737] (WB, Human)	WB	Human
Sugiyama T, Taniguchi K, Matsuhashi N et al. MiR-133b inhibits growth of human gastric cancer cells by silencing pyruvate kinase muscle-splicer polypyrimidine tract-binding protein 1. Cancer Sci. 2016-12-01 [PMID: 27696637] (WB, Human)	WB	Human
Christensen DR, Calder PC, Houghton FD. GLUT3 and PKM2 regulate OCT4 expression and support the hypoxic culture of human embryonic stem cells. Sci Rep. 2015-12-07 [PMID: 26639784] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Taniguchi K, Sugito N, Kumazaki M et al. Positive feedback of DDX6/c-Myc/PTB1 regulated by miR-124 contributes to maintenance of the Warburg effect in colon cancer cells Biochim. Biophys. Acta. 2015-07-02 [PMID: 26144048] (WB, Human) Details: PKM2 antibody was used for WB assay on lysates of human colon cancer cells (DLD-1 cells and WiDr cells ) transfected with miR-124 or siR-PTB1 (Fig. 3B and 3C). WB was also performed on lysates after the transfection of colon cancer cells with siR-c-Myc or siR-DDX6 (Fig. 4A qand 4B), and on DLD-1 cells /WiDr cells that were subjected to PKM1 and/or PKM2 knockdown using siRNAPKM1 and siRNAPKM2 (Fig. 5B and 5E).	WB	Human
Taniguchi K, Ito Y, Sugito N et al. Organ-specific PTB1-associated microRNAs determine expression of pyruvate kinase isoforms Sci Rep 2015-02-27 [PMID: 25721733] (IHC-P, ICC/IF, WB, Human, Mouse)	IHC-P, ICC/IF, WB	Human, Mouse
Shinohara H, Taniguchi K, Kumazaki M et al. Anti-cancer fatty-acid derivative induces autophagic cell death through modulation of PKM isoform expression profile mediated by bcr-abl in chronic myeloid leukemia Cancer Lett. 2015-01-30 [PMID: 25644089] (WB, Human) Details: PKM2 antibody used for WB on lysates of human leukemia cell lines K562 and KCL-22 cells which were subjected to AIC-47/a fatty-acid derivative treatment for 72?hours (Fig.?3B). WB also performed on the lysates of K562 and KCL-22 cells at 72?h after transfection of the cells with control RNA or siRNAs against c-Myc, PTB1 or PKM2 (Fig.?3C, 3D and 3E ), and with control RNA or bcr-abl-specific siRNA /siR-bcr-abl (Fig.?4). PKM1 WB also performed on lysates of K562 cells transfected with control siRNA or siRNA-STAT3 (Figure S1A) and cells treated or not with Rapamycin (Figure S2).	WB	Human
Bruns Dr, Brown Rd, Stenmark Kr et al. Mitochondrial Integrity in a neonatal Bovine Model of Right Ventricular Dysfunction. Am. J. Physiol. Lung Cell Mol. Physiol. 2014-11-21 [PMID: 25416385]
Lindsay Kj, Du J, Sloat Sr et al. Pyruvate kinase and aspartate-glutamate carrier distributions reveal key metabolic links between neurons and glia in retina. Proc. natl. Acad. Sci. U.S.A. 2014-10-28 [PMID: 25313047]
Tan Shen Mynn, Altschuler Gabriel, Zhao Tian Yun et al. Divergent LIN28-mRNA associations result in translational suppression upon the initiation of differentiation. Nucleic Acids Res 2014-01-01 [PMID: 24860167] (WB, Human)	WB	Human
Sasaki M, Fujimoto S, Sato Y et al. Reduction of reactive oxygen species ameliorates metabolism-secretion coupling in islets of diabetic GK rats by suppressing lactate overproduction. Diabetes 2013-01-24 [PMID: 23349483]
Luo W, Hu H, Chang R et al. Pyruvate Kinase M2 is a PHD3-Stimulated Coactivator for Hypoxia-Inducible Factor 1. Cell;145(5):732-44. 2011-05-27 [PMID: 21620138] (WB, IP, Human)	WB, IP	Human
Show All 17 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Sachin Puri	ICC	Human	06/15/2017	View Summary Application Immunocytochemistry Sample Tested Human lung cancer cell lines Species Human
Enlarge	5 reviewed by: Kelsey Stockbridge	WB	Human	02/17/2011	View Summary Application Western Blot Sample Tested HeLa cells Species Human
Enlarge	5 reviewed by: Jian Zhang	IF	Rat	10/15/2010	View Summary Application Immunofluorescence Sample Tested Rat glioblastoma frozen section Species Rat

Array NBP1-48308

• PKM2 Antibodies
• PKM2 ELISA Kits
• PKM2 Lysates
• PKM2 Proteins
• PKM2 Proteins and Enzyme