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PKA C alpha Antibody (7H3A4) - BSA Free

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Western Blot: PKA C alpha Antibody (7H3A4) [NBP2-37246] - Western blot analysis using PRKACA mAb against HEK293 (1) and PRKACA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: PKA C alpha Antibody (7H3A4) [NBP2-37246] - Western blot analysis using PRKACA mAb against human PRKACA (AA: 1-120) recombinant protein. (Expected MW is 39.7 kDa)
ELISA: PKA C alpha Antibody (7H3A4) [NBP2-37246] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
7H3A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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PKA C alpha Antibody (7H3A4) - BSA Free Summary

Immunogen
Purified recombinant fragment of human PKA C alpha (AA: 1-120) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKACA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Western Blot 1:500 - 1:2000
Theoretical MW
40.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PKA C alpha Antibody (7H3A4) - BSA Free

  • cAMP-dependent protein kinase catalytic subunit alpha
  • Cs
  • EC 2.7.11
  • EC 2.7.11.11
  • MGC102831
  • MGC48865
  • PKA C alpha
  • PKA C-alpha
  • Pkaca
  • PKCA1
  • PKCD
  • PRKACA
  • protein kinase A catalytic subunit
  • protein kinase, cAMP-dependent, catalytic, alpha

Background

PKA (Protein Kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKACA