Home » AKAP95/AKAP8 » AKAP95/AKAP8 Antibodies » AKAP95/AKAP8 Antibody (3D4)
Novus Biologicals products are now on bio-techne.com

AKAP95/AKAP8 Antibody (3D4)

 Submit a Review

Images

 
(
Enlarge)
Western Blot: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - AKAP8 monoclonal antibody (M01), clone 3D4 Analysis of AKAP8 expression in Hela S3 NE.
Western Blot: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - AKAP8 monoclonal antibody (M01), clone 3D4 Analysis of AKAP8 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Analysis of monoclonal antibody to AKAP8 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Analysis of monoclonal antibody to AKAP8 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
Western Blot: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Analysis of AKAP8 expression in transfected 293T cell line by AKAP8 monoclonal antibody (M01), clone 3D4.Lane 1: AKAP8 transfected lysate(76.2 KDa).Lane 2: ...read more
Immunoprecipitation: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Analysis of AKAP8 transfected lysate using anti-AKAP8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AKAP8 monoclonal antibody.
Sandwich ELISA: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Detection limit for recombinant GST tagged AKAP8 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clone
3D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

AKAP95/AKAP8 Antibody (3D4) Summary

Immunogen
AKAP8 (NP_005849, 551 a.a. ~ 662 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAES
Marker
Nucleus without Nucleoli Marker
Specificity
AKAP8 - A kinase (PRKA) anchor protein 8
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AKAP8
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AKAP95/AKAP8 Antibody (3D4)

  • A kinase (PRKA) anchor protein 8
  • AKAP-8
  • AKAP-95 AKAP 95
  • A-kinase anchor protein 8
  • A-kinase anchor protein 95 kDa
  • A-kinase anchor protein, 95kDa
  • DKFZp586B1222

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

HA95/AKAP8L
Antibody
NBP2-47440
Immunocytochemistry/ Immunofluorescence HA95/AKAP8L Antibody
Immunohistochemistry-Paraffin HA95/AKAP8L Antibody
Immunohistochemistry-Paraffin HA95/AKAP8L Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AKAP1 Antibody
NBP1-89172
Western Blot AKAP1 Antibody
Immunohistochemistry-Paraffin AKAP1 Antibody
Immunohistochemistry-Paraffin AKAP1 Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
AKAP13 Antibody
NBP1-89163
Immunocytochemistry/ Immunofluorescence AKAP13 Antibody
Immunohistochemistry-Paraffin AKAP13 Antibody
Immunohistochemistry-Paraffin AKAP13 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AKAP5 Antibody -
BSA Free
NBP2-92794
Immunohistochemistry-Paraffin AKAP5 Antibody - BSA Free
Western Blot AKAP5 Antibody - BSA Free
Immunohistochemistry-Paraffin AKAP5 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
PKA R2 Antibody
(OTI1F8)
NBP2-02520
Flow Cytometry PKA R2 Antibody (OTI1F8)
Flow Cytometry PKA R2 Antibody (OTI1F8)
Flow Cytometry PKA R2 Antibody (OTI1F8)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MYCBP Antibody
(2E9)
H00026292-M02
Immunocytochemistry/ Immunofluorescence MYCBP Antibody (2E9)
ELISA MYCBP Antibody (2E9)
Immunohistochemistry-Paraffin MYCBP Antibody (2E9)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
SC35 Antibody
NBP2-47290
Immunohistochemistry-Paraffin SC35 Antibody
Immunohistochemistry-Paraffin SC35 Antibody
Immunohistochemistry-Paraffin SC35 Antibody
(1 Review)
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
HDAC3 Antibody
NB100-1669
Immunocytochemistry/ Immunofluorescence HDAC3 Antibody
Immunocytochemistry/ Immunofluorescence HDAC3 Antibody
Simple Western HDAC3 Antibody
(9 Publications)
Species: Hu
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(291 Publications)
Species: Mu
Applications: ELISA
PDE7A Antibody
NB300-639
Western Blot PDE7A Antibody
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IP, WB
Phosphodiesterase
4A/PDE4A Antibody (OT ...
NBP2-02559
Flow Cytometry Phosphodiesterase 4A/PDE4A Antibody (OTI1C8)
Immunocytochemistry/ Immunofluorescence Phosphodiesterase 4A/PDE4A Antibody (OTI1C8)
Immunohistochemistry-Paraffin Phosphodiesterase 4A/PDE4A Antibody (OTI1C8)
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Lactate
Dehydrogenase A/LDHA ...
NBP1-48336
Immunocytochemistry/ Immunofluorescence Lactate Dehydrogenase A/LDHA Antibody - BSA Free
Simple Western Lactate Dehydrogenase A/LDHA Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Lactate Dehydrogenase A/LDHA Antibody - BSA Free
(4 Reviews)
(43 Publications)
Species: Bv, Hu, Mu, Po
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Cyclin D3 Antibody
(588726)
MAB6570
Western Blot Cyclin D3 Antibody (588726)
Immunohistochemistry Cyclin D3 Antibody (588726)
(1 Publication)
Species: Hu
Applications: IHC, WB
CDK4 Antibody
NBP1-31308
Immunocytochemistry/ Immunofluorescence CDK4 Antibody
Immunocytochemistry/ Immunofluorescence CDK4 Antibody
Western Blot CDK4 Antibody
(7 Publications)
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(807 Publications)
Species: Hu
Applications: BA
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
PKA C alpha/beta
Antibody (515741)
MAB5908
Western Blot PKA C alpha/beta Antibody (515741)
Western Blot PKA C alpha/beta Antibody (515741)
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB
AKAP95/AKAP8
Antibody (3D4)
H00010270-M01
AKAP95/AKAP8 Antibody (3D4)
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IP

Publications for AKAP95/AKAP8 Antibody (H00010270-M01) (0)

There are no publications for AKAP95/AKAP8 Antibody (H00010270-M01).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for AKAP95/AKAP8 Antibody (H00010270-M01) (0)

There are no reviews for AKAP95/AKAP8 Antibody (H00010270-M01). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AKAP95/AKAP8 Antibody (H00010270-M01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional AKAP95/AKAP8 Products

Research Areas for AKAP95/AKAP8 Antibody (H00010270-M01)

Find related products by research area.

Blogs on AKAP95/AKAP8.

Check out the latest blog posts on AKAP95/AKAP8.
AKAP95/AKAP8 Orchestrates and Synchronizes Cellular Events
A-kinase anchor proteins (AKAPs), such as AKAP95/AKAP8, are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA). AKAPs orchestrate and synchronize cellular events by tethering the cAMP-dependent PKA and oth...  Read full blog post.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our AKAP95/AKAP8 Antibody (3D4) and receive a gift card or discount.


Bioinformatics

Gene Symbol AKAP8
Entrez
OMIM
Uniprot