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SP17 Antibody (3G9F3)

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Flow Cytometry: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis of SK-OV-3 cells using SP17 mouse mAb (green) and negative control (red).
Western Blot: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis using SP17 mAb against human SP17 (AA: 1-152) recombinant protein. (Expected MW is 20.3 kDa)
Immunohistochemistry: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis of paraffin-embedded rectal cancer tissues using SP17 mouse mAb with DAB staining.
ELISA: SP17 Antibody (3G9F3) [NBP3-27160] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Flow Cytometry: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis of Hela cells using SP17 mouse mAb (green) and negative control (red).
Immunohistochemistry: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis of paraffin-embedded testis tissues using SP17 mouse mAb with DAB staining.
Western Blot: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis using SP17 mAb against HEK293-6e (1) and SP17 (AA: 1-152)-hIgGFc transfected HEK293-6e (2) cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
3G9F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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SP17 Antibody (3G9F3) Summary

Immunogen
Purified recombinant fragment of human SP17 (AA: 1-152) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPA17
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
17.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SP17 Antibody (3G9F3)

  • Cancer/testis antigen 22
  • CT22SP17-1
  • Sp17-1
  • SP17sperm surface protein Sp17
  • sperm autoantigenic protein 17Sperm protein 17

Background

SP17 encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SPA17