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PKA C beta Antibody

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Western Blot: PKA C beta Antibody [NBP1-55008] - Mouse primary endothelial cell lysates, 20 ug total protein per lane, 4-12% Bis-Tris gel. Antibody at 1:1000. Anti-rabbit HRP conjugated secondary antibody at 1:5000. ...read more
Western Blot: PKA C beta Antibody [NBP1-55008] - PKA C-beta Antibody [NBP1-55008] - Human Lung lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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PKA C beta Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to PRKACB(protein kinase, cAMP-dependent, catalytic, beta) The peptide sequence was selected from the middle region of PRKACB. Peptide sequence NGVSDIKTHKWFATTDWIAIYQRKVEAPFIPKFRGSGDTSNFDDYEEEDI. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKACB
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-55008 in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for PKA C beta Antibody

  • cAMP-dependent protein kinase catalytic beta subunit isoform 4ab
  • cAMP-dependent protein kinase catalytic subunit beta
  • cAPKbeta
  • DKFZp781I2452
  • EC 2.7.11
  • EC 2.7.11.11
  • MGC41879
  • MGC9320
  • PKA C beta
  • PKA C-beta
  • PKACb
  • PRKACB
  • protein kinase A catalytic subunit beta
  • protein kinase, cAMP-dependent, catalytic, beta

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holo

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PKA C beta Antibody (NBP1-55008) (0)

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Review for PKA C beta Antibody (NBP1-55008) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-55008:
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Images Ratings Applications Species Date Details
Western Blot PKA C beta NBP1-55008
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5
reviewed by:
Verified Customer
WB Mouse 11/04/2019
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Summary

ApplicationWestern Blot
Sample TestedAdult lung
SpeciesMouse
LotQC32454

Comments

CommentsProtein content from mouse primary endothelial cells was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody PKA C beta diluted 1:1000 in 5% BSA 2, before incubation with Anti-rabbit secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western Chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PKA C beta Antibody (NBP1-55008) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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Verified Customer
11/04/2019
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PRKACB
Uniprot