Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Western blot analyses of periostin from soleus muscle. The graph shows ...read more
Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin ...read more
Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Analysis of periostin in rabbit periosteum. Image courtesy of product review submitted by Samer Zaky.
Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.
Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Western blot analyses of periostin. The graph shows the quantification of ...read more
Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin ...read more
Genetic Strategies: Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Representative IHC images from 3 separate hearts analyzed for Periostin/OSF-2 protein expression ...read more
Genetic Strategies: Knockout Validated: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Loss of Tgfbi does not alter disease progression after MI. Western blot analysis for TGFBI and ...read more
Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age.(A) Evaluation of cardiac function by transthoracic ...read more
Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - TGFBI & periostin are induced in the heart after injury.(A) Western blot analysis of periostin & TGFBI in isolated adult ...read more
Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - PostnMCM allele activity in vivo.(a) Schematic representation of the Postn genetic locus with a tamoxifen-regulated MerCreMer cDNA ...read more
Product Details
Summary
Reactivity
Hu, Mu, Rt, Ch, Bv, Ca, Eq, Fe, Av-Bi, Gt, Gp, Ha, Pm, Rb, ShSpecies Glossary
Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free Summary
Description
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Immunogen
Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminal region of mouse periostin which is comprised of six small alternatively-spliced exons. Accession # Q62009
Specificity
Specific for the ~93 kDa periostin doublet in mouse lung extract. Consistent with the fact that this antibody is made against an alternatively spliced region of periostin, this antibody recognizes only the largest two of the three forms of periostin recognized on Western blots by the Pan periostin antibody and also shows a distinctive staining pattern by immunohistochemistry. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20).
For IHC-P antigen retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Immunocytochemistry/Immunofluorescence was reported in scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 31430335).
Theoretical MW
93 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4 using NBP1-30042 in the following applications:
Expected reactivity to all mammalian and avian species. Based on customer review rabbit reacts.
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Antigen Affinity-purified
Alternate Names for Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free
Fasciclin I-like
MGC119510
MGC119511
OSF2
OSF-2
OSF-2osteoblast specific factor 2 (fasciclin I-like)
OSF2periodontal ligament-specific periostin
Osteoblast-specific factor 2
PDLPOSTN
periostin isoform thy2
periostin isoform thy4
periostin isoform thy6
periostin isoform thy8
Periostin
periostin, osteoblast specific factor
PNRP11-412K4.1
POSTN
TRIF52
Background
Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Periostin/OSF-2 Antibody (NBP1-30042)(36)
We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.
We have publications tested in 10 applications: Flow Cytometry, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, WB, Western Blot.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Western blot analyses of periostin from soleus muscle. The graph shows quantification of periostin levels normalized to GAPDH (n = 4). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Western-Blot-NBP1-30042-img0008.jpg "Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Western blot analyses of periostin from soleus muscle. The graph shows quantification of periostin levels normalized to GAPDH (n = 4). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.")
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![Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin (red) with DAPI (blue) from soleus muscle. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-30042-img0009.jpg "Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin (red) with DAPI (blue) from soleus muscle. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.")
![Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Analysis of periostin in rabbit periosteum. Image courtesy of product review submitted by Samer Zaky.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Immunohistochemistry-NBP1-30042-img0003.jpg "Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Analysis of periostin in rabbit periosteum. Image courtesy of product review submitted by Samer Zaky.")
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Western-Blot-NBP1-30042-img0004.jpg "Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.")
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Western blot analyses of periostin. The graph shows the quantification of periostin levels normalized to GAPDH (n = 3). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Western-Blot-NBP1-30042-img0007.jpg "Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Western blot analyses of periostin. The graph shows the quantification of periostin levels normalized to GAPDH (n = 3). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin (red) with DAPI (blue) from cardiac muscle. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-30042-img0006.jpg "Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin (red) with DAPI (blue) from cardiac muscle. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.")
![Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Representative IHC images from 3 separate hearts analyzed for Periostin/OSF-2 protein expression within the infarct region of hearts from Postn^MCM/+; R26^EGFP mice versus Postn^MCM/MCM; R26^EGFP mice 2 weeks after MI. Nuclei are shown with DAPI (blue). Scale bars: 20 um. Postn lineage-traced cells (EGFP+) cells are also shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29664017/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2 Antibody - C-terminus - Azide and BSA Free-Immunohistochemistry-NBP1-30042-img0010.jpg "Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Representative IHC images from 3 separate hearts analyzed for Periostin/OSF-2 protein expression within the infarct region of hearts from Postn^MCM/+; R26^EGFP mice versus Postn^MCM/MCM; R26^EGFP mice 2 weeks after MI. Nuclei are shown with DAPI (blue). Scale bars: 20 um. Postn lineage-traced cells (EGFP+) cells are also shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29664017/) licensed under a CC-BY license.")
![Knockout Validated: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Loss of Tgfbi does not alter disease progression after MI. Western blot analysis for TGFBI and periostin from isolated infarct areas of the hearts of the indicated groups of mice 7 days after MI. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0181945), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Knockout-Validated-NBP1-30042-img0005.jpg "Knockout Validated: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Loss of Tgfbi does not alter disease progression after MI. Western blot analysis for TGFBI and periostin from isolated infarct areas of the hearts of the indicated groups of mice 7 days after MI. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0181945), licensed under a CC-BY license.")
![Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age.(A) Evaluation of cardiac function by transthoracic echocardiographic analyses. Left ventricular ejection fractions (LVEFs) are shown (n = 8–9). (B) H&E staining of cryosections from cardiac muscle. (C) Immunostaining of laminin ɑ2 (green) & periostin (red) with DAPI (blue) from cardiac muscle. (D) Western blot analyses of periostin. The graph shows the quantification of periostin levels normalized to GAPDH (n = 3). (E) qPCR analyses of Tgfb2 (TGF beta 2), Postn (periostin), & Fn1 (fibronectin) mRNA levels in cardiac muscle (n = 4). (F) qPCR analyses of Nppa (natriuretic peptide A) & Nppb (natriuretic peptide B) mRNA levels in cardiac muscle (n = 4). In qPCR analyses, data were normalized by Gapdh mRNA & expressed as fold increases from WT mice. ***P<0.001 compared with WT mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31430335), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30042_rabbit-polyclonal-periostin-osf-2-antibody-c-terminus-31020241616035.jpg "Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age.(A) Evaluation of cardiac function by transthoracic echocardiographic analyses. Left ventricular ejection fractions (LVEFs) are shown (n = 8–9). (B) H&E staining of cryosections from cardiac muscle. (C) Immunostaining of laminin ɑ2 (green) & periostin (red) with DAPI (blue) from cardiac muscle. (D) Western blot analyses of periostin. The graph shows the quantification of periostin levels normalized to GAPDH (n = 3). (E) qPCR analyses of Tgfb2 (TGF beta 2), Postn (periostin), & Fn1 (fibronectin) mRNA levels in cardiac muscle (n = 4). (F) qPCR analyses of Nppa (natriuretic peptide A) & Nppb (natriuretic peptide B) mRNA levels in cardiac muscle (n = 4). In qPCR analyses, data were normalized by Gapdh mRNA & expressed as fold increases from WT mice. ***P<0.001 compared with WT mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31430335), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - TGFBI & periostin are induced in the heart after injury.(A) Western blot analysis of periostin & TGFBI in isolated adult cardiomyocytes & fibroblasts. Sarcomeric alpha -actin was used as a control for cardiomyocyte purity & GAPDH was used as a loading control. (B) Quantitative real time PCR for Postn & Tgfbi from 1 week sham or MI-operated hearts. mRNA levels were normalized to 18s ribosomal RNA. *p<0.05 for both genes in MI-operated animals compared to sham animals using an unpaired Student’s T-test. n = 3 animals. (C) Western blot analysis for periostin & TGFBI in the infarcted areas isolated from hearts 24 hours, 7 & 14 days after MI surgery. GAPDH was used as a loading control. Each lane corresponds to protein from one mouse. (D & E) Quantification of TGFBI (D) & periostin (E) protein levels from the conditions shown in panel C except that 3 hearts were analyzed in total each. (F) Immunohistochemistry for the indicated markers/proteins on sham or MI-operated hearts after 1 week. Images were taken at 400x magnification. Scale = 20 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0181945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30042_rabbit-polyclonal-periostin-osf-2-antibody-c-terminus-31020241616068.jpg "Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - TGFBI & periostin are induced in the heart after injury.(A) Western blot analysis of periostin & TGFBI in isolated adult cardiomyocytes & fibroblasts. Sarcomeric alpha -actin was used as a control for cardiomyocyte purity & GAPDH was used as a loading control. (B) Quantitative real time PCR for Postn & Tgfbi from 1 week sham or MI-operated hearts. mRNA levels were normalized to 18s ribosomal RNA. *p<0.05 for both genes in MI-operated animals compared to sham animals using an unpaired Student’s T-test. n = 3 animals. (C) Western blot analysis for periostin & TGFBI in the infarcted areas isolated from hearts 24 hours, 7 & 14 days after MI surgery. GAPDH was used as a loading control. Each lane corresponds to protein from one mouse. (D & E) Quantification of TGFBI (D) & periostin (E) protein levels from the conditions shown in panel C except that 3 hearts were analyzed in total each. (F) Immunohistochemistry for the indicated markers/proteins on sham or MI-operated hearts after 1 week. Images were taken at 400x magnification. Scale = 20 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0181945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - PostnMCM allele activity in vivo.(a) Schematic representation of the Postn genetic locus with a tamoxifen-regulated MerCreMer cDNA cassette inserted into exon 1 (E1), which was crossed with Rosa26 reporter mice (R26-eGFP) containing loxP sites flanking a stop cassette upstream of eGFP to allow for Cre-dependent lineage tracing. (b) Experimental scheme whereby PostnMCM/+; R26-eGFP mice were given tamoxifen for 8 weeks before harvesting at 16 weeks. (c) Representative histological section from the heart of mice described in a & b, which show exceptionally rare labelling of interstitial cells at baseline (arrow) with 8 weeks of tamoxifen. Nuclei are stained in blue. Inset shows alpha -actin stained cardiomyocytes (red) surrounding the one eGFP-labelled interstitial cell (green) (n=4 mice). (d) Experimental scheme whereby PostnMCM/+; R26-eGFP mice were MI injured or subjected to a sham procedure, then given tamoxifen for 1 week before harvesting. (e) Western blot analysis for periostin, eGFP, Cre (MerCreMer protein) & GAPDH as a control at day 0 before injury or day 7 after MI injury with 1 week of tamoxifen (n=3 mice per condition). (f) Representative histological sections showing eGFP-labelled interstitial cells in hearts of PostnMCM/+; R26-eGFP after MI injury with 7 days of tamoxifen labelling, but not with a sham procedure (n=6 mice for MI & n=3 for sham). (g) Whole-mount fluorescent images of hearts from PostnMCM/+; R26-eGFP mice for direct eGFP fluorescence over the given time course shown. A no tamoxifen control 7 days after MI is also shown (n=3 mice per time point & condition). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27447449), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30042_rabbit-polyclonal-periostin-osf-2-antibody-c-terminus-310202416163715.jpg "Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - PostnMCM allele activity in vivo.(a) Schematic representation of the Postn genetic locus with a tamoxifen-regulated MerCreMer cDNA cassette inserted into exon 1 (E1), which was crossed with Rosa26 reporter mice (R26-eGFP) containing loxP sites flanking a stop cassette upstream of eGFP to allow for Cre-dependent lineage tracing. (b) Experimental scheme whereby PostnMCM/+; R26-eGFP mice were given tamoxifen for 8 weeks before harvesting at 16 weeks. (c) Representative histological section from the heart of mice described in a & b, which show exceptionally rare labelling of interstitial cells at baseline (arrow) with 8 weeks of tamoxifen. Nuclei are stained in blue. Inset shows alpha -actin stained cardiomyocytes (red) surrounding the one eGFP-labelled interstitial cell (green) (n=4 mice). (d) Experimental scheme whereby PostnMCM/+; R26-eGFP mice were MI injured or subjected to a sham procedure, then given tamoxifen for 1 week before harvesting. (e) Western blot analysis for periostin, eGFP, Cre (MerCreMer protein) & GAPDH as a control at day 0 before injury or day 7 after MI injury with 1 week of tamoxifen (n=3 mice per condition). (f) Representative histological sections showing eGFP-labelled interstitial cells in hearts of PostnMCM/+; R26-eGFP after MI injury with 7 days of tamoxifen labelling, but not with a sham procedure (n=6 mice for MI & n=3 for sham). (g) Whole-mount fluorescent images of hearts from PostnMCM/+; R26-eGFP mice for direct eGFP fluorescence over the given time course shown. A no tamoxifen control 7 days after MI is also shown (n=3 mice per time point & condition). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27447449), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
