![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - Analysis of 5'-Nucleotidase/CD73 antibody on Human lung tissue. PFA fixed paraffin-embedded sections. Sections were incubated with the primary antibody overnight in 4C (1:200 dilution). Image from verified customer review.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Immunohistochemistry-Paraffin-NBP2-48480-img0006.jpg "Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - Analysis of 5'-Nucleotidase/CD73 antibody on Human lung tissue. PFA fixed paraffin-embedded sections. Sections were incubated with the primary antibody overnight in 4C (1:200 dilution). Image from verified customer review.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, IHC |
| Clone | AD2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Additional Information | Clone AD2 was used by HLDA to establish CD designation. |
| Immunogen | Human CD73 [UniProt# P21589] |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NT5E |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
IHC-P | Human | 01/19/2022 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for 5'-Nucleotidase/CD73 Antibody (NBP2-48480)Find related products by research area.
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Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu... Read full blog post. |
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CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase) CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble... Read full blog post. |
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| Gene Symbol | NT5E |
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on CD3+ human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25186PCP.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Flow-Cytometry-NBP2-48480-img0005.jpg "Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on CD3+ human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25186PCP.")
![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on U87 MG cells with 5'-Nucleotidase/CD73 Antibody (AD2) antibody NBP2-48480 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Flow-Cell-Surface-NBP2-48480-img0001.jpg "Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on U87 MG cells with 5'-Nucleotidase/CD73 Antibody (AD2) antibody NBP2-48480 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.")
![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Flow-Cell-Surface-NBP2-48480-img0002.jpg "Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.")
![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A cell surface stain was performed on U87MG cells with NBP2-48480APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Flow-Cell-Surface-NBP2-48480-img0004.jpg "Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A cell surface stain was performed on U87MG cells with NBP2-48480APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")
