Perilipin-3/TIP47 Antibody

TIP47 has been described as cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. PLIN3 has been localized in milk fat globule membranes of human and bovine origin. It has also been described as a placental protein. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. Therefore, TIP47 is probably significant as an oncodevelopmental marker.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.

Showing Publications 1 - 3 of 3.

Publications using NBP1-87871	Applications	Species
McLelland GL, Lee SA, McBride HM, Fon EA. Syntaxin-17 delivers PINK1/parkin-dependent mitochondrial vesicles to the endolysosomal system J. Cell Biol. 2016-08-01 [PMID: 27458136]
Ioannou MS, Bell ES, Girard M et al. DENND2B activates Rab13 at the leading edge of migrating cells and promotes metastatic behavior J. Cell Biol. 2015-03-02 [PMID: 25713415] (ICC/IF, Human)	ICC/IF	Human
Vogt DA, Camus G, Herker E et al. Lipid Droplet-Binding Protein TIP47 Regulates Hepatitis C Virus RNA Replication through Interaction with the Viral NS5A Protein. PLoS Pathog 2013-01-01 [PMID: 23593007] (ICC/IF)	ICC/IF

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Human	02/19/2019	View Summary Application Western Blot Sample Tested hela cell Species Human Lot A47635 Comments Comments Western blot analysis of cell lysates from HeLa cells transfected with pCi-3xFlag plasmids expressing human Perilipin 3 (PLIN3), human Perilipin 2 (PLIN2), empty vector (mock), and human Vastus lateralis muscle samples (muscle). Proteins were revealed using ABIN4344877 (top) and an anti-Flag-tag antibody (middle) for endogenous and/or recombinant, Flag-tagged PLIN3 and PLIN2. GAPDH (bottom) revealed using a GAPDH antibody served as loading control. The PLIN3 antibody ABIN4344877 reveals a protein of the expected molecular weight of the recombinant PLIN3 in lysates of transfected HeLa cells. It does also detect endogenous PLIN3 in extracts of mock-transfected Hela cells and human muscle tissue. *https://www.antibodies-online.com/antibody/4344877/anti-Perilipin+3+PLIN3+antibody/#tab-validation-566

Array NBP1-87871

• Perilipin-3/TIP47 Antibodies
• Perilipin-3/TIP47 ELISA Kits
• Perilipin-3/TIP47 Lysate
• Perilipin-3/TIP47 Proteins