![Western Blot: OIP106 Antibody [NBP1-56761] - HT1080, Antibody Dilution: 1.0 ug/ml There is BioGPS gene expression data showing that TRAK1 is expressed in HT1080.](http://images.novusbio.com/fullsize/OIP106-Antibody-Western-Blot-NBP1-56761-img0006.jpg "Western Blot: OIP106 Antibody [NBP1-56761] - HT1080, Antibody Dilution: 1.0 ug/ml There is BioGPS gene expression data showing that TRAK1 is expressed in HT1080.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Description | The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution. |
| Immunogen | Synthetic peptides corresponding to TRAK1(trafficking protein, kinesin binding 1) The peptide sequence was selected from the middle region of TRAK1. Peptide sequence ILETEAADLGNDERSKKPGTPGTPGSHDLETALRRLSLRRENYLSERRFF. The peptide sequence for this immunogen was taken from within the described region. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TRAK1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 106 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 2% Sucrose |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: OIP106 Antibody [NBP1-56761] - HT1080 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/OIP106-Antibody-Western-Blot-NBP1-56761-img0002.jpg "Western Blot: OIP106 Antibody [NBP1-56761] - HT1080 cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: OIP106 Antibody [NBP1-56761] - HCT15, Antibody Dilution: 1.0 ug/ml There is BioGPS gene expression data showing that TRAK1 is expressed in HCT15.](http://images.novusbio.com/fullsize/OIP106-Antibody-Western-Blot-NBP1-56761-img0003.jpg "Western Blot: OIP106 Antibody [NBP1-56761] - HCT15, Antibody Dilution: 1.0 ug/ml There is BioGPS gene expression data showing that TRAK1 is expressed in HCT15.")
![Western Blot: OIP106 Antibody [NBP1-56761] - Hela, Antibody Dilution: 1.0 ug/ml TRAK1 is supported by BioGPS gene expression data to be expressed in HeLa.](http://images.novusbio.com/fullsize/OIP106-Antibody-Western-Blot-NBP1-56761-img0004.jpg "Western Blot: OIP106 Antibody [NBP1-56761] - Hela, Antibody Dilution: 1.0 ug/ml TRAK1 is supported by BioGPS gene expression data to be expressed in HeLa.")
![Western Blot: OIP106 Antibody [NBP1-56761] - Analysis of HepG2 cell lysate. Antibody Dilution: 1.0 ug/ml TRAK1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.](http://images.novusbio.com/fullsize/OIP106-Antibody-Western-Blot-NBP1-56761-img0005.jpg "Western Blot: OIP106 Antibody [NBP1-56761] - Analysis of HepG2 cell lysate. Antibody Dilution: 1.0 ug/ml TRAK1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.")
![Western Blot: OIP106 Antibody [NBP1-56761] - Lanes: Lane1 : 10 ug myc-hTRAK1 transfected HEK293 lysate Primary Antibody Dilution: 1 : 500 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1 : 2000 Gene name: TRAK1 Submitted by: Gulcin Pekkurnaz, F. M. Kirby Neurobiology Center.](http://images.novusbio.com/fullsize/OIP106-Antibody-Western-Blot-NBP1-56761-img0007.jpg "Western Blot: OIP106 Antibody [NBP1-56761] - Lanes: Lane1 : 10 ug myc-hTRAK1 transfected HEK293 lysate Primary Antibody Dilution: 1 : 500 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1 : 2000 Gene name: TRAK1 Submitted by: Gulcin Pekkurnaz, F. M. Kirby Neurobiology Center.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")