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dynactin 4 Antibody (3G9D7) - BSA Free

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Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mouse mAb against Raw264.7 (1) and NIH3T3 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Immunofluorescence analysis of HepG2 cells using DCTN4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DCTN4 mouse mAb with DAB staining.
Flow Cytometry: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Flow cytometric analysis of A549 cells using DCTN4 mouse mAb (green) and negative control (red).
Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mAb against human DCTN4 recombinant protein. (Expected MW is 53.2 kDa)
Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mAb against HEK293 (1) and DCTN4 (AA: ***)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using DCTN4 mouse mAb with DAB staining.
ELISA: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
3G9D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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dynactin 4 Antibody (3G9D7) - BSA Free Summary

Immunogen
Purified recombinant fragment of human dynactin 4 (AA: 57-298) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DCTN4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
52.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for dynactin 4 Antibody (3G9D7) - BSA Free

  • dynactin 4 (p62)
  • dynactin p62 subunit
  • dynactin subunit 4
  • Dynactin subunit p62

Background

Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DCTN4