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Nurr1/NGFI-B beta/NR4A2 Antibody

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Immunocytochemistry/Immunofluorescence: Nurr1/NGFI-B beta/NR4A2 Antibody [NBP1-87765] - Staining of human cell line SiHa shows localization to nuclear speckles. Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Nurr1/NGFI-B beta/NR4A2 Antibody [NBP1-87765] - Staining of human cell line SiHa shows localization to nuclear speckles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Nurr1/NGFI-B beta/NR4A2 Antibody [NBP1-87765] - Staining of human kidney shows moderate membranous and nuclear positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Nurr1/NGFI-B beta/NR4A2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VQAQYGSSPQGASPASQSYSYHSSGEYSSDFLTPEFVKFSMDLTNTEITATTSLPSFSTFMDNYSTGYDVKPPCLYQMPLSGQQSSIKVEDIQMHNYQQHSHLPPQSEEMMPHSGSVYYKP
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR4A2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Nurr1/NGFI-B beta/NR4A2 Protein (NBP1-87765PEP)
Publications
Read Publications using NBP1-87765.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Nurr1/NGFI-B beta/NR4A2 Antibody

  • human homolog of
  • NGFI-B beta
  • NOT
  • NR4A2
  • nuclear receptor subfamily 4, group A, member 2
  • Nurr1
  • orphan nuclear receptor NR4A2
  • Orphan nuclear receptor NURR1
  • T-cell nuclear receptor NOT
  • TINUR
  • transcriptionally inducible nuclear receptor related 1
  • Transcriptionally-inducible nuclear receptor

Background

The Nurr1 gene encodes a member of the steroid-thyroid hormone- retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson's. Nurr1 is a stem cell marker and cooperates with PITX3 to promote terminal maturation of murine and human embryonic stem cell cultures to a midbrain dopamine neuron phenotype. In addition, Nurr1 is involved in the regulation of corticotropin-releasing hormone (CRH), which may be linked to and associated with rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nurr1/NGFI-B beta/NR4A2 Antibody (NBP1-87765)(2)


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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NR4A2