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Neurogenin-2 Antibody (7G4) [Unconjugated]

 (14 Publications)
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Neurogenin‑2 was detected in immersion fixed paraffin-embedded sections of human cerebellum using Mouse Anti-Human/Rat Neurogenin‑2 Monoclonal Antibody (Catalog # MAB3314) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neurogenin‑2 was detected in immersion fixed paraffin-embedded sections of human cerebellum using Mouse Anti-Human/Rat Neurogenin‑2 Monoclonal Antibody (Catalog # MAB3314) at 5 µg/ml for 1 hour at room ...read more
Neurogenin‑2 was detected in immersion fixed paraffin-embedded sections of rat brain using Mouse Anti-Human/Rat Neurogenin‑2 Monoclonal Antibody (Catalog # MAB3314) at 5 µg/ml for 1 hour at room ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications IHC
Clone
7G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3314-SP
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Neurogenin-2 Antibody (7G4) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant rat Neurogenin-2
Specificity
Detect human and rat Neurogenin‑2.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NEUROG2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB3314 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurogenin-2 Antibody (7G4) [Unconjugated]

  • Atoh4
  • BHLHA8
  • bHLHa8MGC46562
  • Class A basic helix-loop-helix protein 8
  • Math4A
  • NEUROG2
  • neurogenin 2
  • Neurogenin2
  • Neurogenin-2
  • NGN2
  • ngn-2
  • Protein atonal homolog 4

Background

Neurogenin-2 (NgN2) is a member of neuroD-related bHLH transcription factor family. It is required for neurogenesis and can be used as a marker for cells of neuronal lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neurogenin-2 Antibody (MAB3314)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Immunocytochemistry.


Filter By Application
ICC
(2)
IHC
(4)
IHC-Fr
(1)
IHC-P
(1)
Immunocytochemistry
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using MAB3314 Applications Species
Ozkan, A;Padmanabhan, HK;Shipman, SL;Azim, E;Kumar, P;Sadegh, C;Basak, AN;Macklis, JD; Directed differentiation of functional corticospinal-like neurons from endogenous SOX6+/NG2+ cortical progenitors bioRxiv : the preprint server for biology 2024-04-23 [PMID: 38712174] (Immunocytochemistry, Human) Immunocytochemistry Human
Edwards, N;Combrinck, C;McCaughey-Chapman, A;Connor, B; Directly reprogrammed fragile X syndrome dorsal forebrain precursor cells generate cortical neurons exhibiting impaired neuronal maturation Frontiers in cellular neuroscience 2023-09-21 [PMID: 37810261] (ICC, Human) ICC Human
X Shi, Y Zhuang, Z Chen, M Xu, J Kuang, XL Sun, L Gao, X Kuang, H Zhang, W Li, SZH Wong, C Liu, L Liu, D Jiang, D Pei, Y Lin, QF Wu Hierarchical deployment of Tbx3 dictates the identity of hypothalamic KNDy neurons to control puberty onset Science Advances, 2022-11-16;8(46):eabq2987. 2022-11-16 [PMID: 36383654] (IHC, Mouse) IHC Mouse
Stefan Washausen, Wolfgang Knabe Responses of Epibranchial Placodes to Disruptions of the FGF and BMP Signaling Pathways in Embryonic Mice Frontiers in Cell and Developmental Biology 2021-09-13 [PMID: 34589483]
YH Zhang, M Xu, X Shi, XL Sun, W Mu, H Wu, J Wang, S Li, P Su, L Gong, M He, M Yao, QF Wu Cascade diversification directs generation of neuronal diversity in the hypothalamus Cell Stem Cell, 2021-04-21;0(0):. 2021-04-21 [PMID: 33887179] (IHC, Mouse) IHC Mouse
S Cheng, T Liu, Y Hu, Y Xia, J Hou, C Huang, X Zou, J Liang, Y Stone Shi, Y Zheng, J Lu, G Chen Conditional inactivation of Pen-2 in the developing neocortex leads to rapid switch of apical progenitors to basal progenitors J. Neurosci., 2019-01-28;0(0):. 2019-01-28 [PMID: 30692224] (IHC-P, Mouse) IHC-P Mouse
B Connor, E Firmin, A McCaughey-, R Monk, K Lee, S Liot, J Geiger, C Rudolph, K Jones Conversion of adult human fibroblasts into neural precursor cells using chemically modified mRNA Heliyon, 2018-11-08;4(11):e00918. 2018-11-08 [PMID: 30450440] (ICC, Human) ICC Human
Angelica M. Kowalchuk, Kate A. Maurer, Farnaz Shoja-Taheri, Nadean L. Brown Requirements for Neurogenin2 during mouse postnatal retinal neurogenesis Developmental Biology 2018-10-01 [PMID: 30048641]
Generation of dopamine neuronal-like cells from induced neural precursors derived from adult human cells by non-viral expression of lineage factors Journal of Stem Cells and Regenerative Medicine 2018-05-30 [PMID: 30018471]
Stefan Washausen, Wolfgang Knabe Lateral line placodes of aquatic vertebrates are evolutionarily conserved in mammals Biology Open 2018-06-19 [PMID: 29848488]
Show All 14 Publications.

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Bioinformatics

Gene Symbol NEUROG2