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LMX1b Antibody

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Immunohistochemistry-Paraffin: LMX1b Antibody [NBP1-36970] - (2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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LMX1b Antibody Summary

Immunogen
Peptide with sequence QQALTTSCYFRDRK corresponding to internal region according to NP_002307.1.
Epitope
internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LMX1B
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Preliminary experiments gave an approx. 100 kDa band in mouse kidney lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.6 kDa band according to human NP_002307.1 and to mouse NP_034855.1. The 100 kDa band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-36970.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LMX1b Antibody

  • LIM homeobox transcription factor 1, beta
  • LIM homeobox transcription factor 1-beta
  • LIM/homeobox protein 1.2
  • LIM/homeobox protein LMX1B
  • LMX1.2
  • LMX-1.2
  • MGC138325
  • MGC142051
  • NPS1

Background

LMX1B is a gene that codes for a protein with three isoforms, measuring 402, 395, and 406 amino acids in length with weights of approximately 45, 44, and 45 kDa respectively. LMX1B is necessary for the specification of dorsal limb fate. Current studies are being done on several diseases and disorders related to this gene including Meier-Gorlin syndrome, steroid-resistant nephrotic syndrome, sex reversal, attention deficit hyperactivity disorder, cleft lip/palate, familial Mediterranean fever, genitopatellar syndrome, major depressive disorder, nail dysplasia, short stature, heart block, and neuronitis. LMX1B has also been shown to have interactions with LDB1, TCF3, SSBP3, ALX4, and LDB2 in pathways such as the SIDS susceptibility pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LMX1b Antibody (NBP1-36970)(1)

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Secondary Antibodies

 

Isotype Controls

Additional LMX1b Products

Array NBP1-36970

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Bioinformatics

Gene Symbol LMX1B
Entrez
Uniprot