NRSF Antibody Summary
Immunogen |
The immunogen recognized by this antibody maps to a region between residues 800 and 850 of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978). |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
REST |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry
- Immunohistochemistry-Paraffin 1:500-1:2000
- Immunoprecipitation 2-10 ug/mg lysate
|
Application Notes |
Western Blot Not recommended. Use rabbit anti-REST antibody NB100-757. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Publications |
|
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Buffer |
Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
Preservative |
0.09% Sodium Azide |
Concentration |
1.0 mg/ml |
Purity |
Immunogen affinity purified |
Alternate Names for NRSF Antibody
Background
REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for NRSF Antibody (NB100-756)(1)
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Reviews for NRSF Antibody (NB100-756) (0)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for NRSF Antibody (NB100-756). (Showing 1 - 1 of 1 FAQ).
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The customer is complaining that the antibody NB100-756 could not detect the target band and there is non-specific band in western blotting. Much detailed result is enclosed with the mail, please check it and give us some help.
- Your customer is observing a band at the same size that we observe for NRSF. The molecular weight of larger proteins can be harder to estimate due to poor resolution afforded by molecular weight ladders, and to the greater degree of variability in their migration through SDS-PAGE gels. NRSF is also expressed as a number of different isoforms, which is more than likely what your customer is detecting.
Secondary Antibodies
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Isotype Controls
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