![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [DyLight 650] [NB100-56583C] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-DyLight-650-Flow-Cytometry-NB100-56583C-img0004.jpg "Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [DyLight 650] [NB100-56583C] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.")
| Reactivity | Hu, Rt, RbSpecies Glossary |
| Applications | WB, Flow, IHC, KO |
| Clone | 2J10D7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 650 |
| Description | This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet. |
| Immunogen | A portion of amino acids 150-200 of human NF-kB (p50) was used as the immunogen. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NFKB1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
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![NFkB p105/p50 Antibody (2J10D7) [DyLight 650]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "NFkB p105/p50 Antibody (2J10D7) [DyLight 650]")
Secondary Antibodies |
Isotype Controls |
Research Areas for NFkB p105/p50 Antibody (NB100-56583C)Find related products by research area.
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Nuclear Factor kappa B Signaling in the Immune System Nuclear Factor kappa B (NFkB) binds to the kappa-beta site of the immunoglobulin kappa light chain gene enhancer. Thus NFkB has become one of the widely studied transcription factors in innate and adaptive immune responses. The NFkB family is composed... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NFKB1 |
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [DyLight 650] [NB100-56583C] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-DyLight-650-Flow-Cytometry-NB100-56583C-img0001.jpg "Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [DyLight 650] [NB100-56583C] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.")
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [DyLight 650] [NB100-56583C] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-DyLight-650-Flow-Cytometry-NB100-56583C-img0002.jpg "Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [DyLight 650] [NB100-56583C] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.")
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [DyLight 650] [NB100-56583C] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-DyLight-650-Flow-Cytometry-NB100-56583C-img0003.jpg "Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [DyLight 650] [NB100-56583C] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.")
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