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NFkB p105/p50 Antibody (2J10D7) [FITC]

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Product Details

Summary
Reactivity Hu, Rt, RbSpecies Glossary
Applications WB, Flow, IHC, KO
Clone
2J10D7
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-56583AF350
Alexa Fluor 405
NB100-56583AF405
Alexa Fluor 488
NB100-56583AF488
Alexa Fluor 532
NB100-56583AF532
Alexa Fluor 594
NB100-56583AF594
Alexa Fluor 647
NB100-56583AF647
Alexa Fluor 700
NB100-56583AF700
Alexa Fluor 750
NB100-56583AF750
Biotin
NB100-56583B
CoraFluor 1
NB100-56583CL1
DyLight 350
NB100-56583UV
DyLight 405
NB100-56583V
DyLight 488
NB100-56583G
DyLight 550
NB100-56583R
DyLight 594
NB100-56583DL594
DyLight 650
NB100-56583C
DyLight 680
NB100-56583FR
DyLight 755
NB100-56583IR
HRP
NB100-56583H
Janelia Fluor 525
NB100-56583JF525
Janelia Fluor 549
NB100-56583JF549
Janelia Fluor 646
NB100-56583JF646
Janelia Fluor 669
NB100-56583JF669
Unconjugated
NB100-56583
mFluor Violet 450 SE
NB100-56583MFV450
mFluor Violet 500 SE
NB100-56583MFV500
mFluor Violet 610 SE
NB100-56583MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-56583
BSA Free
NB100-56583
BSA Free
NB100-56583SS
Azide and BSA Free
NBP2-80872
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NFkB p105/p50 Antibody (2J10D7) [FITC] Summary

Immunogen
A portion of amino acids 150-200 of human NF-kB (p50) was used as the immunogen.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NFKB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%). Rat reactivity reported in scientific literature (PMID:31850807).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for NFkB p105/p50 Antibody (2J10D7) [FITC]

  • DKFZp686C01211
  • DNA binding factor KBF1
  • DNA-binding factor KBF1
  • EBP-1
  • KBF1
  • NF-kappaB
  • NF-kappa-B
  • NF-kappabeta
  • NFKB-p105
  • NFKB-p50
  • nuclear factor kappa-B DNA binding subunit
  • nuclear factor NF-kappa-B p105 subunit
  • nuclear factor NF-kappa-B p50 subunit
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151
  • p105
  • p50

Background

NFkB is a transcription regulator that is activated by various intra and extra cellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Serine 529 is located in a negatively charged region (amino acids 422-540) that is phosphorylated in response to phorbol myristate acetate plus calcium ionophore activation. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NFkB p105/p50
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NFkB p105/p50 Antibody (2J10D7) [FITC]
Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, KO
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Nuclear Factor kappa B Signaling in the Immune System
Nuclear Factor kappa B (NFkB) binds to the kappa-beta site of the immunoglobulin kappa light chain gene enhancer. Thus NFkB has become one of the widely studied transcription factors in innate and adaptive immune responses. The NFkB family is composed...  Read full blog post.
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Bioinformatics

Gene Symbol NFKB1