![Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].](http://images.novusbio.com/fullsize/NBS1-Antibody-Immunohistochemistry-Paraffin-NBP1-06609-img0003.jpg "Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Nbs1 Antibody was made to a synthetic peptide made to an internal portion of the mouse NBS1 protein (within residues 350-400). [Swiss-Prot# Q9R207] |
| Localization | Nucleus. Telomere. Note= Localizes to discrete nuclear foci after treatment with genotoxic agents. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NBN |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Western blot a band is seen at ~97 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 84 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 30% Glycerol |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nbs1 Antibody (NBP1-06609)Find related products by research area.
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The MRE11 Complex and DNA Damage Response The maintenance of genome stability depends on the DNA damage response (DDR) which is a complex signaling network including cell cycle checkpoints, DNA repair and damage tolerance pathways. The DDR complex has the ability to sense DNA damage and tra... Read full blog post. |
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NBS1: The DNA Repair Trigger NBS1 (Nijmegen breakage syndrome protein 1) is a component of the MRN complex (Mre11-Rad50-Nbs1) that plays important role in detecting DNA double strand breaks (DSBs) and triggering the downstream cascade. DSBs can be caused by ionizing radiation, ch... Read full blog post. |
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NBS1: DNA Repair Trigger NBS1 (Nijmegen breakage syndrome protein 1) is a component of the MRN complex (Mre11-Rad50-Nbs1) that plays important role in detecting DNA double strand breaks (DSBs) and triggering the downstream cascade. DSBs can be caused by ionizing radiation, ch... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NBN |
![Immunohistochemistry: Nbs1 Antibody [NBP1-06609] - Immunohistochemical staining of NBS1 proteins. Representative examples of typical nuclear staining of NBS1(c) scored as high expression in tumor cells. Correspondingly, examples scored as low expression (f) is shown (40x magnification). Image collected and cropped by CiteAb from the following publication (bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4776-9) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-NBP1-06609-img0005.jpg "Immunohistochemistry: Nbs1 Antibody [NBP1-06609] - Immunohistochemical staining of NBS1 proteins. Representative examples of typical nuclear staining of NBS1(c) scored as high expression in tumor cells. Correspondingly, examples scored as low expression (f) is shown (40x magnification). Image collected and cropped by CiteAb from the following publication (bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4776-9) licensed under a CC-BY license.")
![Western Blot: Nbs1 Antibody [NBP1-06609] - Analysis of Nbs1 in NIH/3T3 (Lane 4) and HeLa whole cell extract (Lane 5) with Nbs1 Antibody [NBP1-06609]. Observed molecular weight at ~99 kDa.](http://images.novusbio.com/fullsize/NBS1-Antibody-Western-Blot-NBP1-06609-img0004.jpg "Western Blot: Nbs1 Antibody [NBP1-06609] - Analysis of Nbs1 in NIH/3T3 (Lane 4) and HeLa whole cell extract (Lane 5) with Nbs1 Antibody [NBP1-06609]. Observed molecular weight at ~99 kDa.")
![Immunohistochemistry: Nbs1 Antibody - BSA Free [NBP1-06609] - Immunohistochemical staining of MRE11, RAD50 & NBS1 proteins. Staining for each protein was scored as high or low as described in the Methods section. Representative examples of typical nuclear staining of MRE11 (a), RAD50 (b), & NBS1(c) scored as high expression in tumor cells. Correspondingly, examples of those scored as low expression for MRE11 (d), RAD50 (e), & NBS1 (f) are shown (40× magnification) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30176843), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06609_rabbit-polyclonal-nbs1-antibody-31020241517139.jpg "Immunohistochemistry: Nbs1 Antibody - BSA Free [NBP1-06609] - Immunohistochemical staining of MRE11, RAD50 & NBS1 proteins. Staining for each protein was scored as high or low as described in the Methods section. Representative examples of typical nuclear staining of MRE11 (a), RAD50 (b), & NBS1(c) scored as high expression in tumor cells. Correspondingly, examples of those scored as low expression for MRE11 (d), RAD50 (e), & NBS1 (f) are shown (40× magnification) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30176843), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")