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MYCBP2 Antibody

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Western Blot: MYCBP2 Antibody [NB100-97841] - Whole cell lysate (5, 15, 50 ug loaded) from HeLa cells probed with anti-MYCBP2 antibody at 0.04 ug/ml.
Western Blot: MYCBP2 Antibody [NB100-97841] - Whole cell lysate (5, 15, 50 ug loaded) from HeLa cells probed with anti-MYCBP2 antibody at 0.04 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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MYCBP2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 2425 and 2475 of human protein associated with Myc using the numbering given in entry NP_055872.3 (GeneID 23077).
Localization
Nucleus
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYCBP2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
Application Notes
IP Not recommended, use rabbit anti-PAM antibody NB100-97840.
Theoretical MW
510 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MYCBP2 Antibody

  • DKFZp686M08244
  • EC 6.3.2.-
  • FLJ10106
  • FLJ21646
  • KIAA0916FLJ13826
  • MYC binding protein 2
  • Myc-binding protein 2
  • Pam/highwire/rpm-1 protein
  • PAMFLJ21597
  • probable E3 ubiquitin-protein ligase MYCBP2
  • Protein associated with Myc

Background

In addition to its possible functions as a facilitator of Myc activated transcription, PAM (protein associated with Myc) has also been shown to play a role in mTOR signaling. PAM functions as an E3 ubiquitin ligase for tuberin (TSC2), a negative regulator of the mTOR pathway. Additionally PAM has been shown to function as a guanosine exchange factor (GEF) for Rheb and to facilitate sphingosine-1-phosphate signaling in the mTOR pathway. Alternative names for PAM include Myc-binding protein 2, MYCBP2, Pam/highwire/rpm-1 protein, and KIAA0916.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYCBP2
Entrez
Uniprot