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MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free

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Protein Array: MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free [NBP3-14120] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MEF2D Antibody (PCRP-MEF2D-3A4).
Protein Array: MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free [NBP3-14120] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MEF2D Antibody (PCRP-MEF2D-3A4).
Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MEF2D antibody (PCRP-MEF2D-3A4). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IP, MA
Clone
PCRP-MEF2D-3A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-14120AF350
Alexa Fluor 405
NBP3-14120AF405
Alexa Fluor 488
NBP3-14120AF488
Alexa Fluor 532
NBP3-14120AF532
Alexa Fluor 594
NBP3-14120AF594
Alexa Fluor 647
NBP3-14120AF647
Alexa Fluor 700
NBP3-14120AF700
Alexa Fluor 750
NBP3-14120AF750
Allophycocyanin
NBP3-14120APC
Biotin
NBP3-14120B
CoraFluor 1
NBP3-14120CL1
DyLight 350
NBP3-14120UV
DyLight 405
NBP3-14120V
DyLight 488
NBP3-14120G
DyLight 550
NBP3-14120R
DyLight 594
NBP3-14120DL594
DyLight 650
NBP3-14120C
DyLight 680
NBP3-14120FR
DyLight 755
NBP3-14120IR
FITC
NBP3-14120F
HRP
NBP3-14120H
Janelia Fluor 525
NBP3-14120JF525
Janelia Fluor 549
NBP3-14120JF549
Janelia Fluor 646
NBP3-14120JF646
Janelia Fluor 669
NBP3-14120JF669
PE
NBP3-14120PE
PerCP
NBP3-14120PCP
Unconjugated
NBP3-13967-100ug
mFluor Violet 450 SE
NBP3-14120MFV450
mFluor Violet 500 SE
NBP3-14120MFV500
mFluor Violet 610 SE
NBP3-14120MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-13967-100ug
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MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13967).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant full-length human MEF2D protein (Uniprot: Q14814)
Localization
Nucleus, Translocated by HDAC4 to nuclear dots
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MEF2D
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Immunoprecipitation 1-2 ug per 100-500 ug protein
  • Protein Array
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Theoretical MW
55.94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react in Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free

  • DKFZp686I1536
  • MADS box transcription enhancer factor 2, polypeptide D (myocyte enhancerfactor 2D)
  • MEF2D/DAZAP1 fusion
  • myocyte enhancer factor 2D
  • myocyte enhancer factor 2D/deleted in azoospermia associated protein 1 fusionprotein
  • myocyte-specific enhancer factor 2D

Background

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free
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Bioinformatics

Gene Symbol MEF2D