MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free Summary
Description |
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13967).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
Immunogen |
Recombinant full-length human MEF2D protein (Uniprot: Q14814) |
Localization |
Nucleus, Translocated by HDAC4 to nuclear dots |
Isotype |
IgG2b |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
MEF2D |
Purity |
Protein A or G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 1-2 ug/million cells
- Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
- Immunohistochemistry-Paraffin 1-2 ug/ml
- Immunoprecipitation 1-2 ug per 100-500 ug protein
- Protein Array
|
Application Notes |
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate) |
Theoretical MW |
55.94 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Predicted to react in Mouse and Rat.
Packaging, Storage & Formulations
Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Buffer |
10mM PBS |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
Protein A or G purified |
Alternate Names for MEF2D Antibody (PCRP-MEF2D-3A4) - Azide and BSA Free
Background
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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