Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Rat Tissue Lysates. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat ...read more
Expression of Tagged Proteins: Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MD-2 recombinant protein per lane. Antibodies: MD-2, NBP1-77201 ...read more
Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Daudi Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat ...read more
Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Mouse Spleen Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: MD-2, NBP1-77201 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation ...read more
Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Mouse Tissue Lysates. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat ...read more
Immunocytochemistry/ Immunofluorescence: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Human Spleen Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling MD-2 with ...read more
Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] - Validation in Mouse Spleen Tissue LysateLoading: 15 ug of lysates per lane.Antibodies: MD-2, (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% ...read more
Immunohistochemistry: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MD-2 antibody at 2.5 ug/ml. ...read more
Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] - Validation in Rat Tissue Lysates.Loading: 15 ug of lysates per lane.Antibodies: MD-2, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Rat Spleen Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed rat spleen tissue labeling MD-2 with at ...read more
Immunohistochemistry: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen using anti-MD-2 antibody at 2 ug/ml. Tissue was ...read more
Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] - Validation in Rat Spleen Tissue Lysate.Loading: 15 ug of lysates per lane.Antibodies: MD-2, (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% ...read more
Antibody was raised against a peptide corresponding to 13 amino acids near the center of human MD-2. The immunogen is located within the last 50 amino acids of MD-2. Amino Acid Sequence: CSFKGIKFSKGKYK
Specificity
Human MD-2 has 2 isoforms, including isoform 1 (160aa, 18kD) and isoform 2 (130aa, 15kD). Mouse MD-2 has one isoform (160aa, 18kD) and Rat MD-2 also has one isoform (160aa, 18kD). NBP1-77201 can detect human, mouse and rat.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LY96
Purity
Peptide affinity purified
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19 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified
Alternate Names for MD-2 Antibody - BSA Free
ESOP1
ESOP-1
LY96
ly-96
lymphocyte antigen 96
MD2
MD-2
myeloid differentiation protein-2
Protein MD-2
Background
MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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