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MAP3K9 Antibody

Images

 
Western Blot: MAP3K9 Antibody [NBP1-33704] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K9 antibody diluted by 1:500.
Immunocytochemistry/ Immunofluorescence: MAP3K9 Antibody [NBP1-33704] - Analysis of paraformaldehyde-fixed A549, using MAP3K9 (NBP1-33704) antibody at 1:200 dilution.
Western Blot: MAP3K9 Antibody [NBP1-33704] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE, antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MAP3K9 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MAP3K9. The exact sequence is proprietary.
Predicted Species
Mouse (99%), Rat (97%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP3K9
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for MAP3K9 Antibody

  • EC 2.7.11
  • EC 2.7.11.25
  • MEKK9
  • mitogen-activated protein kinase kinase kinase 9
  • MLK1Mixed lineage kinase 1
  • PRKE1mixed lineage kinase 1 (tyr and ser/thr specificity)

Background

MLK1 is a MLK(MAP3K) type protein kinase. In vitro studies suggest that it is a member of the neuronal apoptotic JNK/c-Jun pathway acting between Rac1/Cdc42 and MKK4 and -7 in death signaling. MLK1 expression has been documented in human epithelial tumor cell lines of colonic, breast and esophageal origin. ESTs have been isolated from several tissue libraries, including normal pancreas and spleen and cancerous pancreas and bladder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP3K9